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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei21 – 2111D-inositol 3-phosphateUniRule annotation
Binding sitei35 – 351UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei90 – 9011D-inositol 3-phosphateUniRule annotation
Binding sitei123 – 12311D-inositol 3-phosphateUniRule annotation
Binding sitei147 – 14711D-inositol 3-phosphateUniRule annotation
Binding sitei167 – 16711D-inositol 3-phosphateUniRule annotation
Binding sitei241 – 2411UDP-GlcNAcUniRule annotation
Binding sitei246 – 2461UDP-GlcNAcUniRule annotation
Binding sitei307 – 3071UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi316 – 3161Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi317 – 3171Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi319 – 3191Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei329 – 3291UDP-GlcNAcUniRule annotation
Binding sitei337 – 3371UDP-GlcNAcUniRule annotation
Metal bindingi343 – 3431MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:SACE_6971
OrganismiSaccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeSaccharopolyspora
Proteomesi
  • UP000006728 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 433433D-inositol 3-phosphate glycosyltransferasePRO_0000400153Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi405948.SeryN2_010100007682.

Structurei

3D structure databases

ProteinModelPortaliA4FQ08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni27 – 282UDP-GlcNAc bindingUniRule annotation
Regioni32 – 3761D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

A4FQ08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTATTDLSRT WPRRAAVLSL HTSPLEQPGT GDAGGMNVYI AKTSAKLAEL
60 70 80 90 100
GTEVEIFTRA TSSDIPPVAQ LAPGVTVRSL LAGPFEGLDK NDLPSQICAF
110 120 130 140 150
AAGALRVEAR HDPGYYDVVH SHYWLSGQVG WLARQRWGVP LVHTAHTLAK
160 170 180 190 200
VKNAALAEGD TPEPMVRVMG EEQVVAGADR LVANTEFEAA DLIERYDADP
210 220 230 240 250
EAVATIPPGV DLERFTPGDR SAARAEFGLP ADAVVLGFVG RIQPLKAPDV
260 270 280 290 300
LLKATAALLD RHPGLRERLV VLVVGGPSGS GLERPRALHE LARSLGITDV
310 320 330 340 350
VRFLPPQRGD ALASVYRACD VVAVPSYSES FGLVALEAQA CGTPVVAAAV
360 370 380 390 400
GGLPVAVADG VSGLLVDGHE TRRWADALAS VVLSPGRRAR LAAGTTGHAA
410 420 430
GFSWESTTEA LLDTYARART TFDARLRIRE VTA
Length:433
Mass (Da):45,712
Last modified:April 17, 2007 - v1
Checksum:iAE6A48A83DAA703E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM06133.1.
RefSeqiWP_009943956.1. NZ_ABFV01000011.1.

Genome annotation databases

EnsemblBacteriaiCAM06133; CAM06133; SACE_6971.
KEGGisen:SACE_6971.
PATRICi23421408. VBISacEry28377_6982.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM06133.1.
RefSeqiWP_009943956.1. NZ_ABFV01000011.1.

3D structure databases

ProteinModelPortaliA4FQ08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi405948.SeryN2_010100007682.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAM06133; CAM06133; SACE_6971.
KEGGisen:SACE_6971.
PATRICi23421408. VBISacEry28377_6982.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_SACEN
AccessioniPrimary (citable) accession number: A4FQ08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: April 17, 2007
Last modified: September 7, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.