A4FPX3 (GSA_SACEN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase
Short name=GSA
EC=5.4.3.8

Alternative name(s):
Glutamate-1-semialdehyde aminotransferase
Short name=GSA-AT

Gene names

Name:	hemL
Ordered Locus Names:	SACE_6935

Organism

Saccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]

Taxonomic identifier

405948 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Saccharopolyspora › Saccharopolyspora erythraea

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-4-amino-5-oxopentanoate = 5-aminolevulinate. HAMAP-Rule MF_00375
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. HAMAP-Rule MF_00375
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00375.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.
Sequence caution	The sequence CAM06098.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: HAMAP protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutamate-1-semialdehyde 2,1-aminomutase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transaminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Glutamate-1-semialdehyde 2,1-aminomutase HAMAP-Rule MF_00375		PRO_0000382365
Amino acid modifications
	Modified residue	279	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4FPX3 [UniParc]. Last modified September 1, 2009. Version 2. Checksum: F66AC6D28566FFA6	FASTA	448	46,064
	10 20 30 40 50 60 MTAANPASTP NPESAPRSRE LFDRALAVTP GGVNSPVRAF HSVGGTPRFM VRGEGTYLWD 70 80 90 100 110 120 ADDNRYVDLV SSWGPMINGH AHPDVVRAVR QAAAGGLSFG TPGVGEIDLA AEIIDRVGPV 130 140 150 160 170 180 EQVRLVNSGT EATMSAVRLA RGFTGRRKVV KFAGCYHGHV DALLASAGSG VATLGLPTTP 190 200 210 220 230 240 GVTGAQAADT IVLPYNDLDA VRRTFAEYGD EIACVITEAA AGNMGAIAPA PGFNGGLREI 250 260 270 280 290 300 TSSAGALLVM DEVMTGFRVS AAGWFGIDGV AGDLYTFGKV MSGGLPAAAF GGRADVMERL 310 320 330 340 350 360 APSGPVYQAG TLAGNPVAVA AGLANLRAAT PEVYAALDRN AERLGELLGS ALSAEGVPHQ 370 380 390 400 410 420 VAFAGNLVSV FFSEDPVRDY AGAQAQQSWR FPPFFHALLE RGIYPPPSAF EAWFVNAAMD 430 440 DAAFDAIAEA LPHAAKAAAA ATEPGAGA

References

[1]

"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338." Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F. Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NRRL 23338.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM420293 Genomic DNA. Translation: CAM06098.1. Different initiation.
RefSeq	YP_001109023.1. NC_009142.1.
3D structure databases
ProteinModelPortal	A4FPX3.
ModBase	Search...
Protein-protein interaction databases
STRING	405948.SACE_6935.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAM06098; CAM06098; SACE_6935.
GeneID	4946667.
KEGG	sen:SACE_6935.
PATRIC	23421332. VBISacEry28377_6944.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0001.
HOGENOM	HOG000020210.
KO	K01845.
Enzyme and pathway databases
UniPathway	UPA00251; UER00317.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 2 hits.
HAMAP	MF_00375. HemL_aminotrans_3.
InterPro	IPR004639. 4pyrrol_synth_GluAld_NH2Trfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR11986. PTHR11986. 1 hit. PTHR11986:SF5. PTHR11986:SF5. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00713. hemL. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSA_SACEN

Accession

Primary (citable) accession number: A4FPX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	September 1, 2009
Last modified:	May 1, 2013
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program