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A4FN94 (A4FN94_SACEN) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 HAMAP MF_00172
EC=2.1.1.14 HAMAP MF_00172
Alternative name(s):
Cobalamin-independent methionine synthase 2 HAMAP MF_00172
Methionine synthase, vitamin-B12 independent isozyme 2 HAMAP MF_00172
Gene names
Name:metE EMBL CAM05519.1
Synonyms:metE2 HAMAP MF_00172
Ordered Locus Names:SACE_6349
OrganismSaccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]
Taxonomic identifier405948 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Saccharopolyspora

Protein attributes

Sequence length762 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172 SAAS SAAS013215

Catalytic activity

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172 SAAS SAAS013215

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172 SAAS SAAS013215

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family. HAMAP MF_00172

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding6481Zinc By similarity HAMAP MF_00172
Metal binding6501Zinc By similarity HAMAP MF_00172
Metal binding7331Zinc By similarity HAMAP MF_00172

Sequences

Sequence LengthMass (Da)Tools
A4FN94 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: 5A3BA2851B93587F

FASTA76283,407
        10         20         30         40         50         60 
MSSRIGSTVL GHPRIGPRRE LKRALEGYWA SRSTEAELRE VARSLRAGTW RELAAAGLDS 

        70         80         90        100        110        120 
VPGNTFSYYD QVLDTAATFG AVPRRFAELG LGPLDTYFAM ARGVESTPPL EMTKWFDTNY 

       130        140        150        160        170        180 
HYLVPEIGPG TTFSLADRTA VEDYREAAAD GVGTRPVVVG PLTFLLLSKS AEDAPKSFRP 

       190        200        210        220        230        240 
LDKLDELVQR YAELLTELHD AGAGWVQLDE PAFAADRTPE ELELLRGAYR TLSQLDRRPD 

       250        260        270        280        290        300 
ILVATYFGDP GDALPTLAST DVEGIAVDLV SNASTVDSLA RVDGLRSKTL VSGLVDGRNV 

       310        320        330        340        350        360 
WRTDLDAALG TAAALLEVAG EVAVSTSCSL LHVPYDVDAE ANLDPHVRPW LAFAKQKAAE 

       370        380        390        400        410        420 
VVTLGRALRE GRDSVADELA AARNAVQDRR SSERLRDNRV RGRLDALSAD SYRRSPYDAR 

       430        440        450        460        470        480 
RAAQRRTLDL PPLPTTTIGS FPQTPDVRKA RAALRNGSID QAAYDQRMLE EISRVIALQE 

       490        500        510        520        530        540 
ALGLDVLVHG EPERNDMVQY FAEHLDGFVS TENGWVQSYG SRCVRPPILF GDVSRPEPIT 

       550        560        570        580        590        600 
TRWAERAQRL TEKPVKGMLT GPITILAWSF VRDDQPLGET AAQVALAIRD EVRDLEAAGI 

       610        620        630        640        650        660 
RVIQVDEPAL RELLPLRAEQ HEDYLDWAVG SFRLATSGVA DRTQVHTHLC YSEFGEVIDA 

       670        680        690        700        710        720 
IVALDADVTS IEAARSRMEV LDDLNAVGFG RGVGPGVYDI HSPRVPSTEE IEGLLAAALR 

       730        740        750        760 
SVPAERLWVN PDCGLKTRGY AEVEPALRSM VEAAARMRAD LA

« Hide

References

[1]"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
Nat. Biotechnol. 25:447-453(2007) [PubMed: 17369815] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM420293 Genomic DNA. Translation: CAM05519.1.
RefSeqYP_001108444.1. NC_009142.1.

3D structure databases

ProteinModelPortalA4FN94.
SMRA4FN94. Positions 7-759.
ModBaseSearch...

Protein-protein interaction databases

STRINGA4FN94.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4946079.
GenomeReviewsGene locus SACE_6349 in contig AM420293_GR.
KEGGsen:SACE_6349.
PATRIC23420084. VBISacEry28377_6328.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0620.
HOGENOMHBG287495.
OMAMLDTAVM.
PhylomeDBA4FN94.
ProtClustDBPRK05222.

Enzyme and pathway databases

BioCycSERY405948:SACE_6349-MONOMER.

Family and domain databases

HAMAPMF_00172. Meth_synth.
[Tree]
InterProIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]
KOK00549.
PfamPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsTIGR01371. Met_syn_B12ind. 1 hit.
ProtoNetSearch...

Entry information

Entry nameA4FN94_SACEN
AccessionPrimary (citable) accession number: A4FN94
Entry history
Integrated into UniProtKB/TrEMBL: April 17, 2007
Last sequence update: April 17, 2007
Last modified: December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info