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A4FN47 (SYR_SACEN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Arginine--tRNA ligase

EC=6.1.1.19
Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS
Gene names
Name:argS
Ordered Locus Names:SACE_6300
OrganismSaccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]
Taxonomic identifier405948 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora

Protein attributes

Sequence length553 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00123

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00123.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

arginine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 553553Arginine--tRNA ligase HAMAP-Rule MF_00123
PRO_1000018110

Regions

Motif130 – 14011"HIGH" region HAMAP-Rule MF_00123

Sequences

Sequence LengthMass (Da)Tools
A4FN47 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: CEF50AAF3D41852D

FASTA55360,089
        10         20         30         40         50         60 
MTPAALAELL RNTAVDVLAS RDLDTSVIPA AVTVERPRNP EHGDYATNIA MQVAKKAGVA 

        70         80         90        100        110        120 
PRDLAGWLAE ALTGQDGIDS VEVAGPGFLN LRLAADAQGQ ILREVLDATD AYGRGAKYQG 

       130        140        150        160        170        180 
LKVNLEFVSA NPTGPVHLGG TRWAAVGDAL GRTLEAQGAE VTREYYFNDA GAQIDRFVRS 

       190        200        210        220        230        240 
LIAAARGEAA PEDGYGGAYI GEIANQVLEA EPTALDLPEA ERHETFRRIG VGLMFDEVKR 

       250        260        270        280        290        300 
SLHDFGVDFD VYFHEDSLHT SGAVEKSVEQ LKESGHLYFA DGAWWLRSTE FGDDKDRVLI 

       310        320        330        340        350        360 
KSDGTTAYIA GDVAYLRDKR NRGFDLCIYM LGADHHGYIP RLKAAAAAFD DDPDAVEVLI 

       370        380        390        400        410        420 
GQMVNLVKDG KPVRMSKRAG TVLRLEDLVD AVGVDAARYS LIRSSVDSAL DIDLDLISKR 

       430        440        450        460        470        480 
SNENPVFYVQ YAHARLSSLQ RNAASLGIDR GSVADADLSL LTHEREGDLI RTLGEYPRVV 

       490        500        510        520        530        540 
RSAAELREPH RIARYLEDLA SAYHKFYDAC RVLQPGDDQA TPLTIARLQL CEAARQVLAN 

       550 
GLGLLGVSAP EQM 

« Hide

References

[1]"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NRRL 23338.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM420293 Genomic DNA. Translation: CAM05472.1.
RefSeqYP_001108397.1. NC_009142.1.

3D structure databases

ProteinModelPortalA4FN47.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING405948.SACE_6300.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAM05472; CAM05472; SACE_6300.
GeneID4946031.
KEGGsen:SACE_6300.
PATRIC23419984. VBISacEry28377_6279.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0018.
HOGENOMHOG000247214.
KOK01887.
OMANPNGPLH.
OrthoDBEOG6JB13C.
ProtClustDBPRK01611.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPMF_00123. Arg_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERPTHR11956. PTHR11956. 1 hit.
PfamPF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSPR01038. TRNASYNTHARG.
SMARTSM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsTIGR00456. argS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYR_SACEN
AccessionPrimary (citable) accession number: A4FN47
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 17, 2007
Last modified: April 16, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries