A4FMQ9 (A4FMQ9_SACEN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase 2 HAMAP MF_00012
Short name=DAD 2 HAMAP MF_00012
EC=4.2.1.9 HAMAP MF_00012

Gene names

Name:	ilvD EMBL CAM05334.1
Synonyms:	ilvD2 HAMAP MF_00012
Ordered Locus Names:	SACE_6161

Organism

Saccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]

Taxonomic identifier

405948 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Saccharopolyspora

Protein attributes

Sequence length	614 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family. HAMAP MF_00012

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00012
Ligand	4Fe-4S HAMAP MF_00012 Iron Iron-sulfur Metal-binding
Molecular function	Lyase HAMAP MF_00012 EMBL CAM05334.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012
	Metal binding	197	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012

Sequences

	Sequence		Length	Mass (Da)
	A4FMQ9 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: D20AE1EDD8886740	FASTA	614	65,293
	10 20 30 40 50 60 MPQLRSRTTT HGRNAAGARS LWRATGMTDD DFGKPIVAIA NSYTQFVPGH VHLRDLGDVV 70 80 90 100 110 120 AETIREAGGV PREFHTIAVD DGIAMGHGGM LYSLPSRELI ADSVEYMVNA HQADALVCIS 130 140 150 160 170 180 NCDKITPGML NAAMRLNVPT VFVSGGPMEA GKAVVVEGVA QAPTDLITTI AASANAAVDD 190 200 210 220 230 240 EGLSEVERSA CPTCGSCSGM FTANSMNCLT EALGLALPGN GSTLATHAAR RALFQEAGRT 250 260 270 280 290 300 VVRIAKRWYQ EDDESVLPRS VASKEAFENA MALDMAMGGS TNTVLHTLAA AREGEIDFTL 310 320 330 340 350 360 QDIEDISRRV PCLSKVSPNS DYHMEDVHRA GGITAILGEL DRAGMLHRDV PSVHSANLTE 370 380 390 400 410 420 WLERWDIRGA SPSPEAVELF HAAPGGVRTT KAFSTDNRWS SLDTDAEGGC IRDVAHAYTA 430 440 450 460 470 480 DGGLAVLRGN LAEDGAVVKT AGVEEELWSF QGPARVLESQ EQAVSAILNR EIQPGEVLVI 490 500 510 520 530 540 RYEGPAGGPG MQEMLHPTAF LKGSGLGKKC ALITDGRFSG GSSGLSIGHI SPEAANGGTI 550 560 570 580 590 600 GLVENGDQIR IDVRERRLEL LVDEQVLAER RAKMEASEHP WQPVGRERPV TAALRAYARL 610 ATSASYGAVR DVSK

References

[1]

"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338." Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F. Nat. Biotechnol. 25:447-453(2007) [PubMed: 17369815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM420293 Genomic DNA. Translation: CAM05334.1.
RefSeq	YP_001108259.1. NC_009142.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4FMQ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4945893.
GenomeReviews	Gene locus SACE_6161 in contig AM420293_GR.
KEGG	sen:SACE_6161.
PATRIC	23419698. VBISacEry28377_6136.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SQDCRWP.
PhylomeDB	A4FMQ9.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	SERY405948:SACE_6161-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A4FMQ9_SACEN

Accession

Primary (citable) accession number: A4FMQ9

Entry history

Integrated into UniProtKB/TrEMBL:	April 17, 2007
Last sequence update:	April 17, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)