A4FMQ5 (ILVC_SACEN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	SACE_6157

Organism

Saccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]

Taxonomic identifier

405948 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Saccharopolyspora

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_1000050573
Sites
	Active site	109	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	A4FMQ5 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: 2E6A3C1F1BB03F86	FASTA	332	35,710
	10 20 30 40 50 60 MATEIFYDAD ADLGIIQGRK VAVIGYGSQG HAHALSLRDS GADVRIGLPE GSKSRAKAEE 70 80 90 100 110 120 EGLRVLTPAE ASAEADLIMI LAPDTKQRQI YADDIAPNLK SGDALFFGHG FNIRYGLIKP 130 140 150 160 170 180 PSDVDVAMVA PKGPGHLVRR QFVDGKGVPC LIAVEQDASG NAQALALSYA AGIGGARAGV 190 200 210 220 230 240 IKTTFTEETE TDLFGEQAVL CGGASALVQT GFEVLVEAGY QPEIAYFEVL HELKLIVDLM 250 260 270 280 290 300 WEGGIAGQRY SISDTAEYGD LTRGPRVIDA HVKESMRKIL AEVQDGTFAK EWVAEDEAGR 310 320 330 PNFNKLQDQG NAHQIEEVGK KLRSLMSWTQ RP

References

[1]

"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338." Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F. Nat. Biotechnol. 25:447-453(2007) [PubMed: 17369815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NRRL 23338.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM420293 Genomic DNA. Translation: CAM05330.1.
RefSeq	YP_001108255.1. NC_009142.1.
3D structure databases
ProteinModelPortal	A4FMQ5.
SMR	A4FMQ5. Positions 4-329.
ModBase	Search...
Protein-protein interaction databases
STRING	A4FMQ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4945889.
GenomeReviews	Gene locus SACE_6157 in contig AM420293_GR.
KEGG	sen:SACE_6157.
PATRIC	23419690. VBISacEry28377_6132.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0059.
HOGENOM	HBG286525.
OMA	EYANGRG.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	SERY405948:SACE_6157-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_SACEN

Accession

Primary (citable) accession number: A4FMQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 17, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program