A4FMP6 (SYE_SACEN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	SACE_6148

Organism

Saccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]

Taxonomic identifier

405948 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Saccharopolyspora

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000330995
Regions
	Motif	26 – 36	11	"HIGH" region HAMAP MF_00022_B
	Motif	270 – 274	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	273	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4FMP6 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: 7F0EDA63F55C26BF	FASTA	503	55,617
	10 20 30 40 50 60 MSTPEATAEA VSGHEPNRAV RARFCPSPTG TPHVGLVRTA LYNWVFARHN QGKLVFRIED 70 80 90 100 110 120 TDASRDSEES YQALLDALRW LGLDWDEGPE VGGEYGPYRQ SERRDIYADI ARRLLEAGEL 130 140 150 160 170 180 YESFSTPEEV EARHRAAGRD PKLGYDNADR DLTDEQKAAF RAEGRNAVLR LRMPEHDITF 190 200 210 220 230 240 ADLVRGEITF PAGSVPDPVL VRGNGDALYT LTNPVDDALM RITHVLRGED LLSSTPRQIA 250 260 270 280 290 300 LYDALRRIGV TDFTPEFGHL PFVMGEGNKK LSKRDPQSNL FHHRDRGFLP EGLLNYLALL 310 320 330 340 350 360 GWSISEDRDV FTLDEMVEAF EIGRVSSNPA RFDQKKADAI NSAHLRALAP DDFLERVVPY 370 380 390 400 410 420 LVSGGVLPAE PTEEQLATVR AAAPLVQERL IVLSDAVGMM RFLFDGDDFA PDPASAEKAL 430 440 450 460 470 480 GEDARPVLEA AVSALEALPE WTTEAIEAAL KESVVDGLGI KPRKAFAPVR VAVTGRTVSP 490 500 PLYESMELLG REVSLRRLRA PLG

References

[1]

"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338." Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F. Nat. Biotechnol. 25:447-453(2007) [PubMed: 17369815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NRRL 23338.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM420293 Genomic DNA. Translation: CAM05321.1.
RefSeq	YP_001108246.1. NC_009142.1.
3D structure databases
ProteinModelPortal	A4FMP6.
ModBase	Search...
Protein-protein interaction databases
STRING	A4FMP6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4945880.
GenomeReviews	Gene locus SACE_6148 in contig AM420293_GR.
KEGG	sen:SACE_6148.
PATRIC	23419672. VBISacEry28377_6123.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	MAHIPLI.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	SERY405948:SACE_6148-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_SACEN

Accession

Primary (citable) accession number: A4FMP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 17, 2007
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program