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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei273ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:SACE_6148
OrganismiSaccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeSaccharopolyspora
Proteomesi
  • UP000006728 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003309951 – 503Glutamate--tRNA ligaseAdd BLAST503

Proteomic databases

PRIDEiA4FMP6.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi405948.SeryN2_010100009434.

Structurei

3D structure databases

ProteinModelPortaliA4FMP6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi26 – 36"HIGH" regionAdd BLAST11
Motifi270 – 274"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252720.
KOiK01885.
OMAiQLCYMPL.
OrthoDBiPOG091H021W.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A4FMP6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPEATAEA VSGHEPNRAV RARFCPSPTG TPHVGLVRTA LYNWVFARHN
60 70 80 90 100
QGKLVFRIED TDASRDSEES YQALLDALRW LGLDWDEGPE VGGEYGPYRQ
110 120 130 140 150
SERRDIYADI ARRLLEAGEL YESFSTPEEV EARHRAAGRD PKLGYDNADR
160 170 180 190 200
DLTDEQKAAF RAEGRNAVLR LRMPEHDITF ADLVRGEITF PAGSVPDPVL
210 220 230 240 250
VRGNGDALYT LTNPVDDALM RITHVLRGED LLSSTPRQIA LYDALRRIGV
260 270 280 290 300
TDFTPEFGHL PFVMGEGNKK LSKRDPQSNL FHHRDRGFLP EGLLNYLALL
310 320 330 340 350
GWSISEDRDV FTLDEMVEAF EIGRVSSNPA RFDQKKADAI NSAHLRALAP
360 370 380 390 400
DDFLERVVPY LVSGGVLPAE PTEEQLATVR AAAPLVQERL IVLSDAVGMM
410 420 430 440 450
RFLFDGDDFA PDPASAEKAL GEDARPVLEA AVSALEALPE WTTEAIEAAL
460 470 480 490 500
KESVVDGLGI KPRKAFAPVR VAVTGRTVSP PLYESMELLG REVSLRRLRA

PLG
Length:503
Mass (Da):55,617
Last modified:April 17, 2007 - v1
Checksum:i7F0EDA63F55C26BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM05321.1.
RefSeqiWP_011874967.1. NZ_ABFV01000013.1.

Genome annotation databases

EnsemblBacteriaiCAM05321; CAM05321; SACE_6148.
KEGGisen:SACE_6148.
PATRICi23419672. VBISacEry28377_6123.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM05321.1.
RefSeqiWP_011874967.1. NZ_ABFV01000013.1.

3D structure databases

ProteinModelPortaliA4FMP6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi405948.SeryN2_010100009434.

Proteomic databases

PRIDEiA4FMP6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAM05321; CAM05321; SACE_6148.
KEGGisen:SACE_6148.
PATRICi23419672. VBISacEry28377_6123.

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252720.
KOiK01885.
OMAiQLCYMPL.
OrthoDBiPOG091H021W.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYE_SACEN
AccessioniPrimary (citable) accession number: A4FMP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 17, 2007
Last modified: November 30, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.