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A4FMM8 (A4FMM8_SACEN) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047
EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL CAM05303.1
Ordered Locus Names:SACE_6130 EMBL CAM05303.1
OrganismSaccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP] EMBL CAM05303.1
Taxonomic identifier405948 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Saccharopolyspora ›

Protein attributes

Sequence length362 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. SAAS SAAS005905 HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. SAAS SAAS005905 HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. SAAS SAAS005905 HAMAP-Rule MF_00047

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. SAAS SAAS005905 HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity SAAS SAAS005905 HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. SAAS SAAS005905 HAMAP-Rule MF_00047

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain148 – 352205ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding176 – 23156ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3061Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3191Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3191Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3211Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
A4FMM8 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: 01F7A0F0A5F6A1C3

FASTA36239,059
        10         20         30         40         50         60 
MTQRKTRVAV VFGGRSTEHG ISCVSAGSVL AHLDRDRFEV VPVGITKEGA WVLAGDDPEA 

        70         80         90        100        110        120 
LEIQDRRTPE ITSGKAVALP GDPTRDLMYL EPGRGGEVLS SVDVVFPVLH GAFGEDGTIQ 

       130        140        150        160        170        180 
GLLEMAGVPY AGPGVLSSAV AMDKEYTKKL LAAEGLAVGT YEVLRRDQST LDQQQRERLG 

       190        200        210        220        230        240 
LPVFVKPARA GSSLGITKVT DHADLDDAIA EARRTDPKVI IESAVTGREV ECGVLEFPDG 

       250        260        270        280        290        300 
RVEASLPAEL RVTGDSTWYD YESKYLDDVT EFDIPAKIGD DAIERMREAA VTAFRALECQ 

       310        320        330        340        350        360 
GLARVDFFVA DDGELIVNEV NTMPGFTAIS LYPRMWAQTG VDYPTLLSTL IETAIARGTG 


LR

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References

[1]"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NRRL 23338.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM420293 Genomic DNA. Translation: CAM05303.1.
RefSeqYP_001108228.1. NC_009142.1.

3D structure databases

ProteinModelPortalA4FMM8.
ModBaseSearch...

Protein-protein interaction databases

STRING405948.SACE_6130.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAM05303; CAM05303; SACE_6130.
GeneID4945859.
KEGGsen:SACE_6130.
PATRIC23419632. VBISacEry28377_6106.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMALLHGPFG.
ProtClustDBPRK01966.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA4FMM8_SACEN
AccessionPrimary (citable) accession number: A4FMM8
Entry history
Integrated into UniProtKB/TrEMBL: April 17, 2007
Last sequence update: April 17, 2007
Last modified: May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info