A4FME5 (RNH2_SACEN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	SACE_6047

Organism

Saccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]

Taxonomic identifier

405948 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Saccharopolyspora

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	Ribonuclease HII HAMAP MF_00052_B		PRO_0000334951
Sites
	Metal binding	17	1	Divalent metal cation By similarity
	Metal binding	18	1	Divalent metal cation By similarity
	Metal binding	111	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4FME5 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: C7778F092E91B841	FASTA	228	24,072
	10 20 30 40 50 60 MQSALDRRGL GPVAGVDEAG RGACAGPLVV AACALKPGDG RRFEGLTDSK VLSPAERERL 70 80 90 100 110 120 YDVIQAKALS LSVIVIPADE VDALGIHVAN LEGMRRAVAQ LSVAPGYVLT DGFRVPGLGA 130 140 150 160 170 180 PSVAVVKGDL VAACVAAASV LAKVTRDRIM TGLHEQFPVY GFDEHKGYCT SDHTARLTEH 190 200 210 220 GPCEEHRWCY ANVVAAAQLH GMRSPRTVSS KPGLFDAFEG DVVDNEQL

References

[1]

"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338." Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F. Nat. Biotechnol. 25:447-453(2007) [PubMed: 17369815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NRRL 23338.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM420293 Genomic DNA. Translation: CAM05220.1.
RefSeq	YP_001108145.1. NC_009142.1.
3D structure databases
ProteinModelPortal	A4FME5.
ModBase	Search...
Protein-protein interaction databases
STRING	A4FME5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4945776.
GenomeReviews	Gene locus SACE_6047 in contig AM420293_GR.
KEGG	sen:SACE_6047.
PATRIC	23419458. VBISacEry28377_6019.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0164.
HOGENOM	HBG584843.
OMA	RLGPTPI.
PhylomeDB	A4FME5.
Enzyme and pathway databases
BioCyc	SERY405948:SACE_6047-MONOMER.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
PANTHER	PTHR10954. RNase_HII/HIII. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_SACEN

Accession

Primary (citable) accession number: A4FME5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 17, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program