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A4FM22

- PNP_SACEN

UniProt

A4FM22 - PNP_SACEN

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Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, SACE_5914
Organism
Saccharopolyspora erythraea (strain NRRL 23338)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi523 – 5231Magnesium By similarity
Metal bindingi529 – 5291Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:SACE_5914
OrganismiSaccharopolyspora erythraea (strain NRRL 23338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora
ProteomesiUP000006728: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 750750Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_0000329825Add
BLAST

Proteomic databases

PRIDEiA4FM22.

Interactioni

Protein-protein interaction databases

STRINGi405948.SACE_5914.

Structurei

3D structure databases

ProteinModelPortaliA4FM22.
SMRiA4FM22. Positions 14-723.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini589 – 64860KH
Add
BLAST
Domaini660 – 72970S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4FM22-1 [UniParc]FASTAAdd to Basket

« Hide

MTDVQSLDEG VYEATAVLDN GAFGTRTVRF ETGRLAKQAA GSVVAYLDDD    50
TMLLSATTAS KQPKEHFDFF PLTVDVEERM YAAGRIPGSF FRREGRPSTD 100
AILTCRLIDR PLRPSFVDGL RNEVQVVVTV MSLDPQDPYD VLAINGASAS 150
TQLSGLPFSG PVGGTRMALI DGQWVAFPTY EQLERAVFDM VVAGRIVGDD 200
VAIMMVEAEA TEKTAGLVSA GAQAPTEEVV AAGLEAAKPF IRTLCQAQQQ 250
LAQAAGKATE EFPVFPAYAD DAFTAVEQAA SAELAEALKI ASKQERENRL 300
DEIKAAVLEK VGTGEGEQFE GREKEVGAAF RSLTKKLVRQ RIIRDQVRID 350
GRGLTDIRSL SAEVGVIPRA HGSALFERGE TQILGVSTLN MLRLEQTIDS 400
LSPETTKRYM HHYNFPPYST GETGRVGSPK RREIGHGALA ERALIPVLPT 450
REEFPYALRQ VSEALGSNGS TSMGSVCAST LSLLNAGVPL KAPVAGIAMG 500
LVSDEIDGKT HYVALTDILG AEDAFGDMDF KVAGTKEFVT ALQLDTKLDG 550
IPSEVLAQAL GQARDARFTI LEVMAEAIGK PDEMSPHAPR VTSISIPVDK 600
IGEVIGPKGK MINSITEETG AEITIEDDGT IYVGAADGPS AEAAIDKINA 650
IANPQLPKVG ERFLGTVVKT AAFGAFVSLL PGKDGLVHIS KLGNGKRIGK 700
VEDVVNVGDK LRVEIADIDS RGKISLVVVD DEAENADKGG ESEETSEQGA 750
Length:750
Mass (Da):79,933
Last modified:April 17, 2007 - v1
Checksum:iBD15D8B21386942C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM420293 Genomic DNA. Translation: CAM05097.1.
RefSeqiYP_001108022.1. NC_009142.1.

Genome annotation databases

EnsemblBacteriaiCAM05097; CAM05097; SACE_5914.
GeneIDi4945653.
KEGGisen:SACE_5914.
PATRICi23419178. VBISacEry28377_5879.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM420293 Genomic DNA. Translation: CAM05097.1 .
RefSeqi YP_001108022.1. NC_009142.1.

3D structure databases

ProteinModelPortali A4FM22.
SMRi A4FM22. Positions 14-723.
ModBasei Search...

Protein-protein interaction databases

STRINGi 405948.SACE_5914.

Proteomic databases

PRIDEi A4FM22.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAM05097 ; CAM05097 ; SACE_5914 .
GeneIDi 4945653.
KEGGi sen:SACE_5914.
PATRICi 23419178. VBISacEry28377_5879.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
    Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
    Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NRRL 23338.

Entry informationi

Entry nameiPNP_SACEN
AccessioniPrimary (citable) accession number: A4FM22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 17, 2007
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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