A4FLX5 (RSMH_SACEN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	SACE_5866

Organism

Saccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]

Taxonomic identifier

405948 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Saccharopolyspora

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000387093
Regions
	Region	50 – 52	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	69	1	S-adenosyl-L-methionine By similarity
	Binding site	103	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	117	1	S-adenosyl-L-methionine By similarity
	Binding site	124	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4FLX5 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: DE4296F91D791032	FASTA	330	35,562
	10 20 30 40 50 60 MADEQQRRAQ DGGSARDRHV PVMMERTLEL LAPALSKGPA VVVDATLGMG GHSEALLAAH 70 80 90 100 110 120 PELTLVGLDR DPDALRLAGE RLAPHSDRVH LVHAVYDEWA EALAGLSLSK VDGALFDLGV 130 140 150 160 170 180 SSLQLDETDR GFAYAHDAPL DMRMDSGAPR TAADVLNTYS AGELTRVLRE YGEEKFAARI 190 200 210 220 230 240 AAAIVRERAK APFDRSGRLV ELLYDAVPAA SRRTGGHPAK RTFQALRIEV NAELEVLGRA 250 260 270 280 290 300 LPAALDSLAV GGRMVVMSYH SLEDRMVKRA FAERAKSKTP VDLPVELPGH GPEIRLLTRG 310 320 330 AELASDAETA ANPRAASVRL RAAERIKEAV

References

[1]

"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338." Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F. Nat. Biotechnol. 25:447-453(2007) [PubMed: 17369815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NRRL 23338.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM420293 Genomic DNA. Translation: CAM05050.1.
RefSeq	YP_001107975.1. NC_009142.1.
3D structure databases
ProteinModelPortal	A4FLX5.
ModBase	Search...
Protein-protein interaction databases
STRING	A4FLX5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4945606.
GenomeReviews	Gene locus SACE_5866 in contig AM420293_GR.
KEGG	sen:SACE_5866.
PATRIC	23419074. VBISacEry28377_5827.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0275.
HOGENOM	HBG302779.
OMA	NPATRAF.
ProtClustDB	PRK00050.
Enzyme and pathway databases
BioCyc	SERY405948:SACE_5866-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_SACEN

Accession

Primary (citable) accession number: A4FLX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	April 17, 2007
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program