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A4FLW2 (A4FLW2_SACEN) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639 RuleBase RU003664
EC=6.3.2.9 HAMAP MF_00639 RuleBase RU003664
Alternative name(s):
D-glutamic acid-adding enzyme HAMAP MF_00639
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase HAMAP MF_00639
Gene names
Name:murD HAMAP MF_00639 EMBL CAM05037.1
Ordered Locus Names:SACE_5853
OrganismSaccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]
Taxonomic identifier405948 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Saccharopolyspora

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639 RuleBase RU003664 SAAS SAAS005762

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639 RuleBase RU003664 SAAS SAAS005762

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639 RuleBase RU003664 SAAS SAAS005762

Subcellular location

Cytoplasm By similarity HAMAP MF_00639 RuleBase RU003664 SAAS SAAS005762.

Sequence similarities

Belongs to the MurCDEF family. HAMAP MF_00639 RuleBase RU003630

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding120 – 1267ATP By similarity HAMAP MF_00639

Sequences

Sequence LengthMass (Da)Tools
A4FLW2 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: 35C682584F132DE5

FASTA46947,605
        10         20         30         40         50         60 
MLVAGAGVSG RSAAEALLAA GASVTVSDAS SDRLVALASL QAQGAELVPG LTEPPEGTDL 

        70         80         90        100        110        120 
VVTSPGWRPE SPLLAATVAA GVELIGEVEL AWRLDQEKAN EPADSTGRPA GPATWLAVTG 

       130        140        150        160        170        180 
TNGKTTTVGM LESILRADGV DVVACGNVGL PVVDAVRAGH RVLAVELSSF QLHWSQTVAP 

       190        200        210        220        230        240 
HASVVLNLAA DHLDWHGSLD AYGAAKARVY AGNSVAVHNA DDEWSVRLAD AGAPQQARRV 

       250        260        270        280        290        300 
SFTLSDPAPG VLGVESGALV DRAFGDRVRL AAVADVHPAG PHNVANALAA ASLARAYGVE 

       310        320        330        340        350        360 
PEAVAEGLRS FQPGAHRSVV VAESAGVSYV DDSKATNPHA ADAALRAHPK VVWIAGGLLK 

       370        380        390        400        410        420 
GADVEDLVAA NAARLAGVVL IGRDRARFAE ALARHAPDVP VREVESGDDA GMLAAVRLAG 

       430        440        450        460 
EFAEPGCAVV LAPAAASMDM FTDYAHRGRA FADAVRDVLS GAVAPANGG

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References

[1]"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
Nat. Biotechnol. 25:447-453(2007) [PubMed: 17369815] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM420293 Genomic DNA. Translation: CAM05037.1.
RefSeqYP_001107962.1. NC_009142.1.

3D structure databases

ProteinModelPortalA4FLW2.
ModBaseSearch...

Protein-protein interaction databases

STRINGA4FLW2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4945593.
GenomeReviewsGene locus SACE_5853 in contig AM420293_GR.
KEGGsen:SACE_5853.
PATRIC23419048. VBISacEry28377_5814.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0771.
HOGENOMHBG750024.
OMAACASWDM.
PhylomeDBA4FLW2.

Enzyme and pathway databases

BioCycSERY405948:SACE_5853-MONOMER.

Family and domain databases

HAMAPMF_00639. MurD.
[Tree]
InterProIPR018109. Folylpolyglutamate_synth_CS.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK01925.
PANTHERPTHR23135:SF2. PTHR23135:SF2. 1 hit.
PfamPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMsTIGR01087. MurD. 1 hit.
PROSITEPS01011. FOLYLPOLYGLU_SYNT_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA4FLW2_SACEN
AccessionPrimary (citable) accession number: A4FLW2
Entry history
Integrated into UniProtKB/TrEMBL: April 17, 2007
Last sequence update: April 17, 2007
Last modified: December 14, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info