A4FLG1 (GCST_SACEN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	SACE_5701

Organism

Saccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]

Taxonomic identifier

405948 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Saccharopolyspora

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: EC transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Aminomethyltransferase HAMAP MF_00259		PRO_1000047695

Sequences

	Sequence		Length	Mass (Da)
	A4FLG1 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: 5B00810A3E950A21	FASTA	367	39,090
	10 20 30 40 50 60 MSSPRQTPLH EIHEALGATF TEFAGWRMPL RYTGDAAEHN AVRTAAGLFD LTHMGEIRIS 70 80 90 100 110 120 GPQAPEALDY ALVANASAIT VGRARYTMIC NSEGGVLDDL IVYRLGEQEY LVVANAANAA 130 140 150 160 170 180 VVSAELAERV ARFEASHEDV SDDYALIAVQ GPKAVDILAP LTSTDLSTVK YYAGYRSEVA 190 200 210 220 230 240 GARVMLARTG YTGEDGFELF TSPADAPAVW QALADSGAEH GLRPAGLSCR DTLRLEAGMP 250 260 270 280 290 300 LYGNELSAEL TPFHANLGRV VKLDKPGDFV GKAPLAAAAE KPTERKLVGL RTDQRRAPRH 310 320 330 340 350 360 GYRVLDAGGA EIGVVTSGAP SPTLGHPIAM AYVDRDHAEP GTALQVDIRG TAVPVEVVAL PFYRRNA

References

[1]

"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338." Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F. Nat. Biotechnol. 25:447-453(2007) [PubMed: 17369815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NRRL 23338.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM420293 Genomic DNA. Translation: CAM04886.1.
RefSeq	YP_001107811.1. NC_009142.1.
3D structure databases
ProteinModelPortal	A4FLG1.
ModBase	Search...
Protein-protein interaction databases
STRING	A4FLG1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4945442.
GenomeReviews	Gene locus SACE_5701 in contig AM420293_GR.
KEGG	sen:SACE_5701.
PATRIC	23418736. VBISacEry28377_5658.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0404.
HOGENOM	HBG299834.
OMA	GWEMPIS.
PhylomeDB	A4FLG1.
ProtClustDB	PRK00389.
Enzyme and pathway databases
BioCyc	SERY405948:SACE_5701-MONOMER.
Family and domain databases
HAMAP	MF_00259. GcvT. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR022903. NH2_Me_Trfase. [Graphical view]
KO	K00605.
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. GcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_SACEN

Accession

Primary (citable) accession number: A4FLG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 17, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program