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A4FL80 (FTHS_SACEN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:SACE_5619
OrganismSaccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]
Taxonomic identifier405948 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora

Protein attributes

Sequence length565 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 565565Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_0000293057

Regions

Nucleotide binding67 – 748ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4FL80 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: 8C202C45707AE1E3

FASTA56559,922
        10         20         30         40         50         60 
MTVPSDLDIS RATALRPLED IAAQLGLGPH LLEPYGHNVA KISLDAIEEL SDRPQARYVV 

        70         80         90        100        110        120 
VSAITPTPLG EGKTTTTVGL GQALRHLGKV SAVAVRQPSM GPTFGIKGGA AGGGYSQVVP 

       130        140        150        160        170        180 
MEALNLHLTG DMHAVTAAHN LLSAMLDNHL HKGNRLGVDP HRITWRRVLD VNDRDLRNIV 

       190        200        210        220        230        240 
TGMGGRADGT PRQTGFDITA ASEVMAVLAL STSLRDMRRR LGRIVVGYTR DGSPVTAEDL 

       250        260        270        280        290        300 
RAAGAMTAIM REAIKPNLMQ TTENTPVLVH AGPFGNIAHG NSSVVADRIA GRCADYLVTE 

       310        320        330        340        350        360 
AGFGADMGAE RFFNIKCRTS GMRPDAAVLV ATVRALKAHS GRYKVVAGRP LPPEMLAENP 

       370        380        390        400        410        420 
DDVLAGAENL RKQIDNIRLH GVSPVVAVNA FPTDHGSEHD AIRRVAEEEG ARVAVSNHYS 

       430        440        450        460        470        480 
EGGKGALELA EVVVEAAEEP NRFRLLYPDS ADLRTKIETI ATRVYGADGV SYQPAAARAL 

       490        500        510        520        530        540 
ADYEAIGFGS LPVCIAKTHL SLSSDPSLLG APTGWTLPVR EVRASIGAGF IYAICGEMRT 

       550        560 
MPGLGSHPAA ERIDIDEHGQ IVGLS 

« Hide

References

[1]"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NRRL 23338.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM420293 Genomic DNA. Translation: CAM04805.1.
RefSeqYP_001107730.1. NC_009142.1.

3D structure databases

ProteinModelPortalA4FL80.
SMRA4FL80. Positions 6-563.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING405948.SACE_5619.

Proteomic databases

PRIDEA4FL80.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAM04805; CAM04805; SACE_5619.
GeneID4945361.
KEGGsen:SACE_5619.
PATRIC23418570. VBISacEry28377_5575.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMACGEIMTM.
OrthoDBEOG6PCPSP.
ProtClustDBPRK13505.

Enzyme and pathway databases

UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_SACEN
AccessionPrimary (citable) accession number: A4FL80
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: April 17, 2007
Last modified: April 16, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways