Skip Header

Contribute Send feedback
Read comments (?) or add your own

A4FIY7 (A4FIY7_SACEN) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 HAMAP MF_00172
EC=2.1.1.14 HAMAP MF_00172
Alternative name(s):
Cobalamin-independent methionine synthase 1 HAMAP MF_00172
Methionine synthase, vitamin-B12 independent isozyme 1 HAMAP MF_00172
Gene names
Name:metE EMBL CAM04012.1
Synonyms:metE1 HAMAP MF_00172
Ordered Locus Names:SACE_4744
OrganismSaccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]
Taxonomic identifier405948 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Saccharopolyspora

Protein attributes

Sequence length761 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172 SAAS SAAS013215

Catalytic activity

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172 SAAS SAAS013215

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172 SAAS SAAS013215

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family. HAMAP MF_00172

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding6471Zinc By similarity HAMAP MF_00172
Metal binding6491Zinc By similarity HAMAP MF_00172
Metal binding7321Zinc By similarity HAMAP MF_00172

Sequences

Sequence LengthMass (Da)Tools
A4FIY7 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: 305FE6B82E889C85

FASTA76183,654
        10         20         30         40         50         60 
MTTKIGSTVL GYPRIGPNRE LKRALESYWA SRSGAEDLRE VARRLRVDTW RTLQDAGLDS 

        70         80         90        100        110        120 
VPGNTFSYYD QVLDTAVTFG AVPPRYTELG LNPLDTYFAM ARGVDSTPPL EMTKWFDTNY 

       130        140        150        160        170        180 
HYLVPEIGPD TRFSLSDRTP VRLFQEAADL GVTTRPVLVG PLTFLLLSKS AEGAPESFHP 

       190        200        210        220        230        240 
LDKLDELLTA YAELLGELKR AGAEWVQLDE PAFAADRSQT ELDALRAAYR KLGELEGRPN 

       250        260        270        280        290        300 
LLVAGYFGDL GDALDVLASA PVDALGVDLV AGSSTVDKLP TLSGLRNKTL VAGLVDGRNI 

       310        320        330        340        350        360 
WRTDLDKALT TGATLLGLAG EVAVSTSCSL LHVPYDVEAE ERIDPQVKSW LAFAVQKVRE 

       370        380        390        400        410        420 
VVVLGRALRE GRDAVADELA AAAAVADRAK ADRVRNNHVR ARLQSLRPEH SRRSDYALRR 

       430        440        450        460        470        480 
EAQEKSLRLP PLPTTTIGSF PQTTDVRKAR AELRNGTIDE AAYEQRMRDE IQRVIELQEG 

       490        500        510        520        530        540 
LGLDVLVHGE PERNDMVQYF SELMEGFVST HKGWVQSYGS RCVRPPILFG DVSRPDPMTV 

       550        560        570        580        590        600 
EWATYAQKLT DKPVKGMLTG PVTILAWSFV RDDQPLADTA GQVALAIRDE VRDLESAGIR 

       610        620        630        640        650        660 
VVQVDEPALR ELLPLRSVRH EEYLDWAVES FRLATSGVAD STQIHTHLCY SEFGDVINAI 

       670        680        690        700        710        720 
VALDADVTSI EAARSRMEVL ADLNAVGFAR GVGPGVYDIH SPRVPETEEV TALLRAALEA 

       730        740        750        760 
VPAQRLWVNP DCGLKTRGYT EVEAALKNVV SATAAVRPTL R

« Hide

References

[1]"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
Nat. Biotechnol. 25:447-453(2007) [PubMed: 17369815] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM420293 Genomic DNA. Translation: CAM04012.1.
RefSeqYP_001106937.1. NC_009142.1.

3D structure databases

ProteinModelPortalA4FIY7.
ModBaseSearch...

Protein-protein interaction databases

STRINGA4FIY7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4944562.
GenomeReviewsGene locus SACE_4744 in contig AM420293_GR.
KEGGsen:SACE_4744.
PATRIC23416960. VBISacEry28377_4774.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0620.
HOGENOMHBG287495.
OMARNIWRAN.
PhylomeDBA4FIY7.
ProtClustDBPRK05222.

Enzyme and pathway databases

BioCycSERY405948:SACE_4744-MONOMER.

Family and domain databases

HAMAPMF_00172. Meth_synth.
[Tree]
InterProIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]
KOK00549.
PfamPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsTIGR01371. Met_syn_B12ind. 1 hit.
ProtoNetSearch...

Entry information

Entry nameA4FIY7_SACEN
AccessionPrimary (citable) accession number: A4FIY7
Entry history
Integrated into UniProtKB/TrEMBL: April 17, 2007
Last sequence update: April 17, 2007
Last modified: December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info