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Protein

Tryptophan 2,3-dioxygenase

Gene

kynA

Organism
Saccharopolyspora erythraea (strain NRRL 23338)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring.UniRule annotation

Catalytic activityi

L-tryptophan + O2 = N-formyl-L-kynurenine.UniRule annotation

Cofactori

hemeUniRule annotationNote: Binds 2 heme groups per tetramer.UniRule annotation

Pathway: L-tryptophan degradation via kynurenine pathway

This protein is involved in step 1 of the subpathway that synthesizes L-kynurenine from L-tryptophan.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Tryptophan 2,3-dioxygenase (kynA)
  2. no protein annotated in this organism
This subpathway is part of the pathway L-tryptophan degradation via kynurenine pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-kynurenine from L-tryptophan, the pathway L-tryptophan degradation via kynurenine pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei112 – 1121SubstrateUniRule annotation
Binding sitei116 – 1161SubstrateUniRule annotation
Binding sitei123 – 1231HemeUniRule annotation
Metal bindingi239 – 2391Iron (heme axial ligand)UniRule annotation
Binding sitei253 – 2531SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Tryptophan catabolism

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00333; UER00453.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan 2,3-dioxygenaseUniRule annotation (EC:1.13.11.11UniRule annotation)
Short name:
TDOUniRule annotation
Alternative name(s):
Tryptamin 2,3-dioxygenaseUniRule annotation
Tryptophan oxygenaseUniRule annotation
Short name:
TOUniRule annotation
Short name:
TRPOUniRule annotation
Tryptophan pyrrolaseUniRule annotation
TryptophanaseUniRule annotation
Gene namesi
Name:kynAUniRule annotation
Ordered Locus Names:SACE_4055
OrganismiSaccharopolyspora erythraea (strain NRRL 23338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeSaccharopolyspora
ProteomesiUP000006728 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 281281Tryptophan 2,3-dioxygenasePRO_0000360132Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi405948.SeryN2_010100017951.

Structurei

3D structure databases

ProteinModelPortaliA4FH01.
SMRiA4FH01. Positions 22-281.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 275Substrate bindingUniRule annotation
Regioni50 – 545Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the tryptophan 2,3-dioxygenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3483.
HOGENOMiHOG000221583.
KOiK00453.
OMAiQYREIEF.
OrthoDBiEOG6WMHXR.

Family and domain databases

HAMAPiMF_01972. T23O.
InterProiIPR004981. Trp_2_3_dOase.
[Graphical view]
PANTHERiPTHR10138. PTHR10138. 1 hit.
PfamiPF03301. Trp_dioxygenase. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4FH01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHGNSRPLE KDVVRDLRRE LSYGQYLRLD RLLDAQHPVS EPEHHDELLF
60 70 80 90 100
IIQHQTVELW LKLILHELRT AREHLARDEL KPALKQLARV KHVQHTLTEQ
110 120 130 140 150
WSVLATLTPA EYVEFRGFLG RSSGFQSYQY RAIELILGNK NAEMLEVFAH
160 170 180 190 200
DEFAHELLNG LLKEPSVYDE FVRLLHRRGH DVPAAFLQRD VSLPHTFTPE
210 220 230 240 250
LVPLFRGIYE TAAENWDAYE ACEELVDLEE NFQFWRFRHL KTVERTIGLK
260 270 280
HGTGGSSGVS FLRRALELTF FPELYAVRTE I
Length:281
Mass (Da):32,858
Last modified:April 17, 2007 - v1
Checksum:i46D47E07ABE1CE8E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM03326.1.
RefSeqiWP_011874224.1. NC_009142.1.
YP_001106251.1. NC_009142.1.

Genome annotation databases

EnsemblBacteriaiCAM03326; CAM03326; SACE_4055.
KEGGisen:SACE_4055.
PATRICi23415556. VBISacEry28377_4072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM03326.1.
RefSeqiWP_011874224.1. NC_009142.1.
YP_001106251.1. NC_009142.1.

3D structure databases

ProteinModelPortaliA4FH01.
SMRiA4FH01. Positions 22-281.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi405948.SeryN2_010100017951.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAM03326; CAM03326; SACE_4055.
KEGGisen:SACE_4055.
PATRICi23415556. VBISacEry28377_4072.

Phylogenomic databases

eggNOGiCOG3483.
HOGENOMiHOG000221583.
KOiK00453.
OMAiQYREIEF.
OrthoDBiEOG6WMHXR.

Enzyme and pathway databases

UniPathwayiUPA00333; UER00453.

Family and domain databases

HAMAPiMF_01972. T23O.
InterProiIPR004981. Trp_2_3_dOase.
[Graphical view]
PANTHERiPTHR10138. PTHR10138. 1 hit.
PfamiPF03301. Trp_dioxygenase. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
    Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
    Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NRRL 23338.

Entry informationi

Entry nameiT23O_SACEN
AccessioniPrimary (citable) accession number: A4FH01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: April 17, 2007
Last modified: June 24, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.