Reviewed, UniProtKB/Swiss-Prot A4FH01 (T23O_SACEN)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase

Gene names

Name:	kynA
Ordered Locus Names:	SACE_4055

Organism

Saccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]

Taxonomic identifier

405948 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Saccharopolyspora

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Tryptophan 2,3-dioxygenase		PRO_0000360132
Regions
	Region	23 – 27	5	Substrate binding By similarity
	Region	50 – 54	5	Substrate binding By similarity
Sites
	Metal binding	239	1	Iron (heme axial ligand) By similarity
	Binding site	112	1	Substrate By similarity
	Binding site	116	1	Substrate By similarity
	Binding site	123	1	Heme By similarity
	Binding site	253	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4FH01-1 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: 46D47E07ABE1CE8E	FASTA	281	32,858
	10 20 30 40 50 60 MVHGNSRPLE KDVVRDLRRE LSYGQYLRLD RLLDAQHPVS EPEHHDELLF IIQHQTVELW 70 80 90 100 110 120 LKLILHELRT AREHLARDEL KPALKQLARV KHVQHTLTEQ WSVLATLTPA EYVEFRGFLG 130 140 150 160 170 180 RSSGFQSYQY RAIELILGNK NAEMLEVFAH DEFAHELLNG LLKEPSVYDE FVRLLHRRGH 190 200 210 220 230 240 DVPAAFLQRD VSLPHTFTPE LVPLFRGIYE TAAENWDAYE ACEELVDLEE NFQFWRFRHL 250 260 270 280 KTVERTIGLK HGTGGSSGVS FLRRALELTF FPELYAVRTE I

References

[1]

"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338." Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F. Nat. Biotechnol. 25:447-453(2007) [PubMed: 17369815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM420293 Genomic DNA. Translation: CAM03326.1.
RefSeq	YP_001106251.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4FH01.
Genome annotation databases
GeneID	4943876.
GenomeReviews	Gene locus SACE_4055 in contig AM420293_GR.
KEGG	sen:SACE_4055.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	QWSVLAT.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_SACEN

Accession

Primary (citable) accession number: A4FH01

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	April 17, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)