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A4FFX3

- MDH_SACEN

UniProt

A4FFX3 - MDH_SACEN

Protein

Malate dehydrogenase

Gene

mdh

Organism
Saccharopolyspora erythraea (strain NRRL 23338)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (17 Apr 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei93 – 931SubstrateUniRule annotation
    Binding sitei99 – 991SubstrateUniRule annotation
    Binding sitei106 – 1061NADUniRule annotation
    Binding sitei113 – 1131NADUniRule annotation
    Binding sitei132 – 1321SubstrateUniRule annotation
    Binding sitei163 – 1631SubstrateUniRule annotation
    Active sitei188 – 1881Proton acceptorUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi12 – 187NADUniRule annotation
    Nucleotide bindingi130 – 1323NADUniRule annotation

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
    Gene namesi
    Name:mdhUniRule annotation
    Ordered Locus Names:SACE_3674
    OrganismiSaccharopolyspora erythraea (strain NRRL 23338)
    Taxonomic identifieri405948 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora
    ProteomesiUP000006728: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 328328Malate dehydrogenasePRO_0000294406Add
    BLAST

    Proteomic databases

    PRIDEiA4FFX3.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi405948.SACE_3674.

    Structurei

    3D structure databases

    ProteinModelPortaliA4FFX3.
    SMRiA4FFX3. Positions 3-328.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0039.
    HOGENOMiHOG000220953.
    KOiK00024.
    OMAiAFSQECI.
    OrthoDBiEOG6PP9Q2.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_01517. Malate_dehydrog_2.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4FFX3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQAPVTVTV TGAAGQIGYA LLFRIASGQL IGPDTPVRLR LLEIPQAVKA    50
    AEGTAMELDD CAFPLLQGIE VTDDARTAFD GTNVALLVGA RPRTKGMERG 100
    DLLEANGGIF KPQGEAINAG AADDVRVLVV GNPANTNALI AQAHAPDVPA 150
    ERFTAMTRLD HNRALTQLAQ KLGVSVNDIK KLTIWGNHSA TQYPDLFHAE 200
    VNGKVAAEQV DQAWLADTFI PTVAKRGAAI IEARGASSAA SAANAAIDHI 250
    HTWVNGTPEG DWTSAAVVSD GSYGVPEGLI SSFPVVARDG RYEIVQGLEI 300
    DEFSRQRIDA SVNELSEERD AVRKLGLL 328
    Length:328
    Mass (Da):34,676
    Last modified:April 17, 2007 - v1
    Checksum:iC3A59357F40E11BC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM420293 Genomic DNA. Translation: CAM02948.1.
    RefSeqiYP_001105873.1. NC_009142.1.

    Genome annotation databases

    EnsemblBacteriaiCAM02948; CAM02948; SACE_3674.
    GeneIDi4943498.
    KEGGisen:SACE_3674.
    PATRICi23414774. VBISacEry28377_3681.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM420293 Genomic DNA. Translation: CAM02948.1 .
    RefSeqi YP_001105873.1. NC_009142.1.

    3D structure databases

    ProteinModelPortali A4FFX3.
    SMRi A4FFX3. Positions 3-328.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 405948.SACE_3674.

    Proteomic databases

    PRIDEi A4FFX3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAM02948 ; CAM02948 ; SACE_3674 .
    GeneIDi 4943498.
    KEGGi sen:SACE_3674.
    PATRICi 23414774. VBISacEry28377_3681.

    Phylogenomic databases

    eggNOGi COG0039.
    HOGENOMi HOG000220953.
    KOi K00024.
    OMAi AFSQECI.
    OrthoDBi EOG6PP9Q2.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_01517. Malate_dehydrog_2.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23382. PTHR23382. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
      Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
      Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NRRL 23338.

    Entry informationi

    Entry nameiMDH_SACEN
    AccessioniPrimary (citable) accession number: A4FFX3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: April 17, 2007
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3