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Reviewed, UniProtKB/Swiss-Prot A4FFQ6 (MTNC_SACEN)

Last modified November 3, 2009. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Enolase-phosphatase E1
    EC=3.1.3.77
Alternative name(s):
    2,3-diketo-5-methylthio-1-phosphopentane phosphatase
Gene names
Name: mtnC
Ordered Locus Names: SACE_3607
OrganismSaccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]
Taxonomic identifier405948 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora

Protein attributes

Sequence length240 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) By similarity.

Catalytic activity

5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate. HAMAP MF_01681

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. HAMAP MF_01681

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.

Subunit structure

Monomer By similarity.

Sequence similarities

Belongs to the HAD-like hydrolase superfamily. MasA/mtnC family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 240240Enolase-phosphatase E1 HAMAP MF_01681
PRO_0000357394

Sequences

Sequence LengthMass (Da)Tools
A4FFQ6-1 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: 07AE1CDDEAF95FB3

FASTA24025,648
        10         20         30         40         50         60 
MSASVAARWV VLDIEGTLTP TSQVHVVLYD YARPRLGPWI HDHPEDPVVR KAVEDVKSEA 

        70         80         90        100        110        120 
GLPAEATAEQ VVAVLHGWMD ADRKAAPLKT LQGLIWQDGY ARGELTTDYF ADVVPALRAW 

       130        140        150        160        170        180 
RQRGLVLAVF SSGSVAGQVA SFSHTTSGDL RGLFAQHFDT VNAGPKRERG SYEAIAAALG 

       190        200        210        220        230        240 
AARPLQIVFL SDVPAELDAA AQAGWHTVGL ARPGEPYADA DFGSHPAVGA FDDIDIEVVS 

« Hide

References

[1]"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
Nat. Biotechnol. 25:447-453(2007) [PubMed: 17369815] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

AM420293 Genomic DNA. Translation: CAM02881.1.
RefSeqYP_001105806.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGA4FFQ6.

Genome annotation databases

GeneID4943431.
GenomeReviewsGene locus SACE_3607 in contig AM420293_GR.
KEGGsen:SACE_3607.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMATTDLNFI.

Family and domain databases

HAMAPMF_01681.
[Tree]
InterProIPR005834. Dehalogen-like_hydro.
IPR010041. Enolase_ppase.
[Graphical view]
PANTHERPTHR20371. Enolase_ppase. 1 hit.
PfamPF00702. Hydrolase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01691. enolase-ppase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMTNC_SACEN
AccessionPrimary (citable) accession number: A4FFQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: April 17, 2007
Last modified: November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents