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Protein

Argininosuccinate synthase

Gene

argG

Organism
Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei99CitrullineUniRule annotation1
Binding sitei129ATP; via amide nitrogenUniRule annotation1
Binding sitei131AspartateUniRule annotation1
Binding sitei131ATPUniRule annotation1
Binding sitei135AspartateUniRule annotation1
Binding sitei135CitrullineUniRule annotation1
Binding sitei136AspartateUniRule annotation1
Binding sitei136ATPUniRule annotation1
Binding sitei139CitrullineUniRule annotation1
Binding sitei192CitrullineUniRule annotation1
Binding sitei194ATPUniRule annotation1
Binding sitei201CitrullineUniRule annotation1
Binding sitei203CitrullineUniRule annotation1
Binding sitei280CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 25ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:SACE_2917
OrganismiSaccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeSaccharopolyspora
Proteomesi
  • UP000006728 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000254401 – 472Argininosuccinate synthaseAdd BLAST472

Proteomic databases

PRIDEiA4FDS0.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi405948.SeryN2_010100028783.

Structurei

3D structure databases

ProteinModelPortaliA4FDS0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230094.
KOiK01940.
OMAiAFHIRSG.
OrthoDBiPOG091H01U0.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2. 1 hit.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4FDS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVLTSLPV GQRVGIAFSG GLDTSVAVAW MRENGAVPCT YTADIGQYDE
60 70 80 90 100
SDIASIPGRA EAYGAEISRL VDCREALVEE GLAALSCGAF HIRSGGRTYF
110 120 130 140 150
NTTPLGRAVT ATLLVRAMLA DDVLIWGDGS TFKGNDIERF YRYGLLANPR
160 170 180 190 200
LRIYKPWLDT AFVGELGGRK EMSEWLQERD LPYRDSAEKA YSTDANIWGA
210 220 230 240 250
THEAKKLEHL DTGIEIVDPI MGVPFWDDSV EIATEDVTLS FVQGRPVAIN
260 270 280 290 300
GKEFDSPVDL VHEANAVGGR HGLGMSDQIE NRIIEAKSRG IYEAPGMALL
310 320 330 340 350
FIAYERLVNA IHNEDTIASY HNEGRRLGRL MYEGRWFDPQ SLMLRESLQR
360 370 380 390 400
WVGSAVTGEV TLRLRRGEDY SILDTTGPAF SYHPDKLSME RTEDAAFGPV
410 420 430 440 450
DRIGQLTMRN LDIADSRAKL ENYAQLGLVG QAQAELVGSL QSGGAEAIAS
460 470
VGTHEESDAG ALDRAAMESG TD
Length:472
Mass (Da):51,621
Last modified:April 17, 2007 - v1
Checksum:iA3B786081009BCDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM02195.1.
RefSeqiWP_009949409.1. NZ_ABFV01000072.1.

Genome annotation databases

EnsemblBacteriaiCAM02195; CAM02195; SACE_2917.
KEGGisen:SACE_2917.
PATRICi23413230. VBISacEry28377_2910.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM02195.1.
RefSeqiWP_009949409.1. NZ_ABFV01000072.1.

3D structure databases

ProteinModelPortaliA4FDS0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi405948.SeryN2_010100028783.

Proteomic databases

PRIDEiA4FDS0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAM02195; CAM02195; SACE_2917.
KEGGisen:SACE_2917.
PATRICi23413230. VBISacEry28377_2910.

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230094.
KOiK01940.
OMAiAFHIRSG.
OrthoDBiPOG091H01U0.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2. 1 hit.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_SACEN
AccessioniPrimary (citable) accession number: A4FDS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 17, 2007
Last modified: October 5, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.