A4FCA0 (A4FCA0_SACEN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Acetyl-coenzyme A synthetase 2 HAMAP MF_01123
EC=6.2.1.1 HAMAP MF_01123

Alternative name(s):
Acetate--CoA ligase 2 HAMAP MF_01123
Acyl-activating enzyme 2 HAMAP MF_01123

Gene names

Name:	acsA EMBL CAM01675.1
Synonyms:	acsA2 HAMAP MF_01123
Ordered Locus Names:	SACE_2375

Organism

Saccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]

Taxonomic identifier

405948 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Saccharopolyspora

Protein attributes

Sequence length	668 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family. HAMAP MF_01123

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_01123 Nucleotide-binding
Molecular function	Ligase HAMAP MF_01123 EMBL CAM01675.1
PTM	Acetylation HAMAP MF_01123
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	533	1	By similarity HAMAP MF_01123
Amino acid modifications
	Modified residue	628	1	N6-acetyllysine By similarity HAMAP MF_01123

Sequences

	Sequence		Length	Mass (Da)
	A4FCA0 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: C7663AB223D18862	FASTA	668	73,110
	10 20 30 40 50 60 MSSKRVERLE VGKHSQNTLD NLLHEERIHA PSGEFAAQAN ATSELYAQAD ADREAFWADQ 70 80 90 100 110 120 ARHLHWDTEW EQVLDWSGAP FAKWFVGGKL NVAYNCVDRH VEAGNGDRVA IHWEGEPGDS 130 140 150 160 170 180 RAITYAELAR EVSRTANALA SLGVGAGDRV AIYLPMLPEA VYSMLACARL GALHSVVFGG 190 200 210 220 230 240 FSSEALRTRI NDAQAKVVIT ADGQYRRGKA MPLKTNVDEA VAATPSVEHV LVVQRTKTDV 250 260 270 280 290 300 EWNDGRDQWW HDVVEGRPAS HTPEFFDSEH PLFILYTSGT TGRPKGILHT SGGYLTQAAY 310 320 330 340 350 360 THRTVFDLKP ETDVYWCTAD IGWVTGHTYI VYGPLANGAT QVIYEGTPNT PHEGRHWDIV 370 380 390 400 410 420 QKYGVTLYYT APTTIRTFMK WGAEIPARYD LSTLRVLGSV GEPINPEAWM WYREHIGGNR 430 440 450 460 470 480 APIVDTWWQT ETGAIMISPL PGVTATKPGS AQVPLPGIAA KVVDESGEQV GHGGGGLLVL 490 500 510 520 530 540 DQPWPAMLRG IWGDDDRYRE TYWSKFADKG YYFAGDGAKY DDDGDIWLLG RVDDVMNVSG 550 560 570 580 590 600 HRISTTEVES ALVSHPTVAE AAVVGASDPT TGQGIVAFVI LRGGADDQAS GEAAIKALRD 610 620 630 640 650 660 HVAHEIGPIA KPRQIMVVPE LPKTRSGKIM RRLLRDVAEN REIGDVSTLA DSSVMNLISA GFATSSDD

References

[1]

"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338." Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F. Nat. Biotechnol. 25:447-453(2007) [PubMed: 17369815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM420293 Genomic DNA. Translation: CAM01675.1.
RefSeq	YP_001104600.1. NC_009142.1.
3D structure databases
ProteinModelPortal	A4FCA0.
SMR	A4FCA0. Positions 29-641.
ModBase	Search...
Protein-protein interaction databases
STRING	A4FCA0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4942224.
GenomeReviews	Gene locus SACE_2375 in contig AM420293_GR.
KEGG	sen:SACE_2375.
PATRIC	23412162. VBISacEry28377_2375.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0365.
HOGENOM	HBG547964.
OMA	HEERIHA.
PhylomeDB	A4FCA0.
ProtClustDB	PRK00174.
Enzyme and pathway databases
BioCyc	SERY405948:SACE_2375-MONOMER.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig. IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01895.
PANTHER	PTHR24095:SF42. PTHR24095:SF42. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A4FCA0_SACEN

Accession

Primary (citable) accession number: A4FCA0

Entry history

Integrated into UniProtKB/TrEMBL:	April 17, 2007
Last sequence update:	April 17, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)