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Protein

Proteasome-associated ATPase

Gene

arc

Organism
Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

ATPase which is responsible for recognizing, binding, unfolding and translocation of pupylated proteins into the bacterial 20S proteasome core particle. May be essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase may function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis.UniRule annotation

Pathwayi: proteasomal Pup-dependent pathway

This protein is involved in the pathway proteasomal Pup-dependent pathway, which is part of Protein degradation.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway proteasomal Pup-dependent pathway and in Protein degradation.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi284 – 289ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00997.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome-associated ATPaseUniRule annotation
Alternative name(s):
AAA ATPase forming ring-shaped complexesUniRule annotation
Short name:
ARCUniRule annotation
Proteasomal ATPaseUniRule annotation
Gene namesi
Name:arcUniRule annotation
Ordered Locus Names:SACE_2246
OrganismiSaccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeSaccharopolyspora
Proteomesi
  • UP000006728 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003970171 – 597Proteasome-associated ATPaseAdd BLAST597

Interactioni

Subunit structurei

Homohexamer. Assembles into a hexameric ring structure that caps the 20S proteasome core. Strongly interacts with the prokaryotic ubiquitin-like protein Pup through a hydrophobic interface; the interacting region of ARC lies in its N-terminal coiled-coil domain. There is one Pup binding site per ARC hexamer ring. Upon ATP-binding, the C-terminus of ARC interacts with the alpha-rings of the proteasome core, possibly by binding to the intersubunit pockets.UniRule annotation

Protein-protein interaction databases

STRINGi405948.SeryN2_010100015018.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni596 – 597Docks into pockets in the proteasome alpha-ringUniRule annotation2

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili21 – 97UniRule annotationAdd BLAST77

Domaini

Consists of three main regions, an N-terminal coiled-coil domain that binds to protein Pup and functions as a docking station, an interdomain involved in ARC hexamerization, and a C-terminal ATPase domain of the AAA type.UniRule annotation

Sequence similaritiesi

Belongs to the AAA ATPase family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4105DHM. Bacteria.
COG0464. LUCA.
HOGENOMiHOG000245286.
KOiK13527.
OMAiCVDEFKE.
OrthoDBiPOG091H02HH.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02112. ARC_ATPase. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
IPR032501. Prot_ATP_ID_OB.
IPR022482. Proteasome_ATPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF16450. Prot_ATP_ID_OB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR03689. pup_AAA. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4FBX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHDRPGSRP EEGGEQQIGG DAELNSQIRL LEDEVALLRR KLNESPQQVR
60 70 80 90 100
LLEKRLAEAS ERVSQLTERN AKLTETLKEA RSQLMALREE VDRLAQPPSG
110 120 130 140 150
YGVFLHAYED STVDVFTSGR KMRVSVSPNV ATEDLRLGQS VRLNEALTVV
160 170 180 190 200
EAGAFEQTGE VCTFRELLPV DAVTRSPRAL VLGHTDEERV VWVTEPLAES
210 220 230 240 250
GLKAGDSVLV DSKAGYAYDL VPKAEVEDLV LEEVPDVGYN DIGGLGNQIE
260 270 280 290 300
QIRDAVELPF LHSDLYVEYQ LRPPKGVLLY GPPGCGKTLI AKAVANSLAK
310 320 330 340 350
KVAASRGDDD GQAKSYFLNI KGPELLNKFV GETERHIRLI FQRAREKASE
360 370 380 390 400
GTPVIVFFDE MDSIFRTRGS GVSSDVETTI VPQLLSEIDG VEGLENVIVI
410 420 430 440 450
GASNREDMID PAILRPGRLD VKIKIERPDA ESAKDIFSKY LTRQLPIHEE
460 470 480 490 500
DLKEFGGDQA SCVEAMIQTT VERMYAETDE NRFLEVTYAN GDKEVLYFKD
510 520 530 540 550
FNSGAMIQNI VDRAKKAAIK AVLETGQPGL RVQHLQDAII DEFAENEDLP
560 570 580 590
NTTNPDDWAR ISGKKGERIV YIRTLVSGKN QESGRAIDTA TNTGQYL
Length:597
Mass (Da):66,124
Last modified:April 17, 2007 - v1
Checksum:i82FE131649202CFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM01551.1.
RefSeqiWP_009945872.1. NZ_ABFV01000024.1.

Genome annotation databases

EnsemblBacteriaiCAM01551; CAM01551; SACE_2246.
KEGGisen:SACE_2246.
PATRICi23411920. VBISacEry28377_2255.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA. Translation: CAM01551.1.
RefSeqiWP_009945872.1. NZ_ABFV01000024.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi405948.SeryN2_010100015018.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAM01551; CAM01551; SACE_2246.
KEGGisen:SACE_2246.
PATRICi23411920. VBISacEry28377_2255.

Phylogenomic databases

eggNOGiENOG4105DHM. Bacteria.
COG0464. LUCA.
HOGENOMiHOG000245286.
KOiK13527.
OMAiCVDEFKE.
OrthoDBiPOG091H02HH.

Enzyme and pathway databases

UniPathwayiUPA00997.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02112. ARC_ATPase. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
IPR032501. Prot_ATP_ID_OB.
IPR022482. Proteasome_ATPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF16450. Prot_ATP_ID_OB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR03689. pup_AAA. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARC_SACEN
AccessioniPrimary (citable) accession number: A4FBX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: April 17, 2007
Last modified: September 7, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.