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A4FBA6

- RUVC_SACEN

UniProt

A4FBA6 - RUVC_SACEN

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Protein
Crossover junction endodeoxyribonuclease RuvC
Gene
ruvC, SACE_2021
Organism
Saccharopolyspora erythraea (strain NRRL 23338)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71Magnesium By similarity
Metal bindingi68 – 681Magnesium By similarity
Metal bindingi141 – 1411Magnesium By similarity
Metal bindingi144 – 1441Magnesium By similarity

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvC (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene namesi
Name:ruvC
Ordered Locus Names:SACE_2021
OrganismiSaccharopolyspora erythraea (strain NRRL 23338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora
ProteomesiUP000006728: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 199199Crossover junction endodeoxyribonuclease RuvCUniRule annotation
PRO_1000002823Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi405948.SACE_2021.

Structurei

3D structure databases

ProteinModelPortaliA4FBA6.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012182.
KOiK01159.
OMAiRQVQEMV.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4FBA6-1 [UniParc]FASTAAdd to Basket

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MRVLGVDPGL TRCGIGVVDG GRGRTVTCVG VGVARTPADQ ELSARLLGVA    50
EAVDEWLDTH RPEVVAIERV FSQHNVRTVM GTAQVSGIVA LAAARRGLPV 100
AFHTPSEVKA AISGSGRADK RQVTTMVTKI LGLAEAPKPA DAADALALAV 150
CHLWRAPMAG RLAEAEAKAA ALARNHKARL KDARQAAAQR AARTGGVQR 199
Length:199
Mass (Da):20,730
Last modified:April 17, 2007 - v1
Checksum:iF9356659725C15B9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM420293 Genomic DNA. Translation: CAM01331.1.
RefSeqiYP_001104256.1. NC_009142.1.

Genome annotation databases

EnsemblBacteriaiCAM01331; CAM01331; SACE_2021.
GeneIDi4941879.
KEGGisen:SACE_2021.
PATRICi23411470. VBISacEry28377_2030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM420293 Genomic DNA. Translation: CAM01331.1 .
RefSeqi YP_001104256.1. NC_009142.1.

3D structure databases

ProteinModelPortali A4FBA6.
ModBasei Search...

Protein-protein interaction databases

STRINGi 405948.SACE_2021.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAM01331 ; CAM01331 ; SACE_2021 .
GeneIDi 4941879.
KEGGi sen:SACE_2021.
PATRICi 23411470. VBISacEry28377_2030.

Phylogenomic databases

eggNOGi COG0817.
HOGENOMi HOG000012182.
KOi K01159.
OMAi RQVQEMV.
OrthoDBi EOG6RG044.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
HAMAPi MF_00034. RuvC.
InterProi IPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view ]
Pfami PF02075. RuvC. 1 hit.
[Graphical view ]
PRINTSi PR00696. RSOLVASERUVC.
SUPFAMi SSF53098. SSF53098. 1 hit.
TIGRFAMsi TIGR00228. ruvC. 1 hit.
PROSITEi PS01321. RUVC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
    Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
    Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NRRL 23338.

Entry informationi

Entry nameiRUVC_SACEN
AccessioniPrimary (citable) accession number: A4FBA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 17, 2007
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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