A4FAQ5 (DXS_SACEN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	SACE_1815

Organism

Saccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]

Taxonomic identifier

405948 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Saccharopolyspora

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 642	642	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000019071

Sequences

	Sequence		Length	Mass (Da)
	A4FAQ5 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: 73947DB60C5D0A76	FASTA	642	68,449
	10 20 30 40 50 60 MTLLESVQSP ADVKRLEHGE LAKLAAEIRS FLIEKVSRTG GHLGPNLGAI ELTLAVHRVF 70 80 90 100 110 120 DSPRDAVVFD TGHQAYVHKI VTGRQSGFDR LRKRDGISGY PSRAESEHDL VENSHASTAL 130 140 150 160 170 180 SYADGLARAF QLGSEQRHVV AVVGDGALTG GMCWEALNNI AADPDRPVVI VVNDNGRSYA 190 200 210 220 230 240 PTIGGLAEHL AGLRLKPGYE RALESGRRTL QNLPVVGRSL YTGLHAAKSG IKDALSPQVM 250 260 270 280 290 300 FSDLGIKYLG PVDGHDLRSM EQALSMAKAF GGPVIVHAVT RKGNGFAPAE NDVAEQMHQV 310 320 330 340 350 360 KVIDPETGIP TKPAPKIWTD VYSDELVRIG AEREDVVAIT AAMLGPTGLD KFAAAYPERC 370 380 390 400 410 420 YDVGIAEQHA MTSAAGMAMG GLHPVFAVYS TFLNRAFDQL LMDVALHRQP VTVTLDRSGI 430 440 450 460 470 480 TGDDGASHNG MWDLSILGAI PGIQVAAPRD AVTLREELRE AVAVDDGPTV MRFPKGSVIE 490 500 510 520 530 540 EVPAVERIGG VDVLSKPASD ERSEVLLAAV GAFGQLGVAV AERLAAQGIG VTVVDPRWVV 550 560 570 580 590 600 PVPQAVLDLA ARHSLVVTLE DCGRHGGFGW SLAAAMRDQE IDVPLRDLAV PNRFLQHASR 610 620 630 640 DEVLSDLGLT EQDIARRITE WVAGRLTPVV VGNGRGETAT GA

References

[1]

"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338." Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F. Nat. Biotechnol. 25:447-453(2007) [PubMed: 17369815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NRRL 23338.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM420293 Genomic DNA. Translation: CAM01130.1.
RefSeq	YP_001104055.1. NC_009142.1.
3D structure databases
ProteinModelPortal	A4FAQ5.
ModBase	Search...
Protein-protein interaction databases
STRING	A4FAQ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4941674.
GenomeReviews	Gene locus SACE_1815 in contig AM420293_GR.
KEGG	sen:SACE_1815.
PATRIC	23411050. VBISacEry28377_1824.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	QYDISYM.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	SERY405948:SACE_1815-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_SACEN

Accession

Primary (citable) accession number: A4FAQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 17, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program