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Protein

Ribonuclease PH

Gene

rph

Organism
Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation.UniRule annotation

Catalytic activityi

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87Phosphate (substrate) bindingUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processrRNA processing, tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease PHUniRule annotation (EC:2.7.7.56UniRule annotation)
Short name:
RNase PHUniRule annotation
Alternative name(s):
tRNA nucleotidyltransferaseUniRule annotation
Gene namesi
Name:rphUniRule annotation
Ordered Locus Names:SACE_1212
OrganismiSaccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeSaccharopolyspora
Proteomesi
  • UP000006728 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000248751 – 251Ribonuclease PHAdd BLAST251

Interactioni

Subunit structurei

Homohexameric ring arranged as a trimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi405948.SeryN2_010100013662

Structurei

3D structure databases

ProteinModelPortaliA4F913
SMRiA4F913
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 127Phosphate (substrate) bindingUniRule annotation3

Sequence similaritiesi

Belongs to the RNase PH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105ED0 Bacteria
COG0689 LUCA
HOGENOMiHOG000229516
KOiK00989
OMAiKGKGQGW
OrthoDBiPOG091H03ML

Family and domain databases

CDDicd11362 RNase_PH_bact, 1 hit
Gene3Di3.30.230.70, 1 hit
HAMAPiMF_00564 RNase_PH, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR015847 ExoRNase_PH_dom2
IPR036345 ExoRNase_PH_dom2_sf
IPR027408 PNPase/RNase_PH_dom_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR002381 RNase_PH_bac-type
IPR018336 RNase_PH_CS
PfamiView protein in Pfam
PF01138 RNase_PH, 1 hit
PF03725 RNase_PH_C, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55666 SSF55666, 1 hit
TIGRFAMsiTIGR01966 RNasePH, 1 hit
PROSITEiView protein in PROSITE
PS01277 RIBONUCLEASE_PH, 1 hit

Sequencei

Sequence statusi: Complete.

A4F913-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARADGRSDD ALREVRITRG YQDWPAGSVL VEFGRTRVLC AASVQEGVPR
60 70 80 90 100
WRAGSGLGWV TAEYAMLPSS TNTRSARESV KGRIGGRTHE ISRLVGRSLR
110 120 130 140 150
ACIDLAALGE NTIALDCDVI QADGGTRTAA ITGAYVALAD AITWLSAAGR
160 170 180 190 200
LTDPKPLSCS IAAVSVGVVD GRVRLDLPYE EDSRAEVDMN VVATDHGTLV
210 220 230 240 250
EVQGTGEGAT FTRSTMDKMI DFALAGCSEL SRIQSEALAA PYPGELPGGA

A
Length:251
Mass (Da):26,399
Last modified:April 17, 2007 - v1
Checksum:i527D789A75ABB6A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM420293 Genomic DNA Translation: CAM00538.1

Genome annotation databases

EnsemblBacteriaiCAM00538; CAM00538; SACE_1212
KEGGisen:SACE_1212

Similar proteinsi

Entry informationi

Entry nameiRNPH_SACEN
AccessioniPrimary (citable) accession number: A4F913
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 17, 2007
Last modified: April 25, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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