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Protein

3-alpha-mycarosylerythronolide B desosaminyl transferase

Gene

eryCIII

Organism
Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of alpha-L-mycarosylerythronolide B into erythromycin D in the erythromycin biosynthesis pathway.1 Publication

Catalytic activityi

dTDP-D-desosamine + 3-alpha-L-mycarosylerythronolide B = dTDP + erythromycin D.1 Publication

Kineticsi

kcat is 1 min(-1).

  1. KM=50 µM for 3-alpha-mycarosylerythronolide B1 Publication

    Pathwayi: erythromycin biosynthesis

    This protein is involved in the pathway erythromycin biosynthesis, which is part of Antibiotic biosynthesis.1 Publication
    View all proteins of this organism that are known to be involved in the pathway erythromycin biosynthesis and in Antibiotic biosynthesis.

    GO - Molecular functioni

    • transferase activity, transferring hexosyl groups Source: UniProtKB

    GO - Biological processi

    • antibiotic biosynthetic process Source: UniProtKB
    • metabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Antibiotic biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17080.
    UniPathwayiUPA00240.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-alpha-mycarosylerythronolide B desosaminyl transferase (EC:2.4.1.278)
    Alternative name(s):
    Desosaminyl transferase EryCIII
    Erythromycin biosynthesis protein CIII
    Gene namesi
    Name:eryCIII
    Ordered Locus Names:SACE_0726
    OrganismiSaccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
    Taxonomic identifieri405948 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeSaccharopolyspora
    Proteomesi
    • UP000006728 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 23Sequence analysisAdd BLAST23
    ChainiPRO_000041849324 – 4213-alpha-mycarosylerythronolide B desosaminyl transferaseAdd BLAST398

    Proteomic databases

    PRIDEiA4F7P3.

    Interactioni

    Subunit structurei

    Heterotetramer composed of EryCII and EryCIII.1 Publication

    Protein-protein interaction databases

    STRINGi405948.SeryN2_010100032633.

    Structurei

    Secondary structure

    1421
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 6Combined sources5
    Helixi11 – 14Combined sources4
    Turni15 – 17Combined sources3
    Helixi18 – 26Combined sources9
    Beta strandi30 – 35Combined sources6
    Helixi37 – 39Combined sources3
    Helixi40 – 44Combined sources5
    Turni45 – 47Combined sources3
    Beta strandi50 – 52Combined sources3
    Helixi59 – 65Combined sources7
    Helixi68 – 74Combined sources7
    Helixi84 – 87Combined sources4
    Helixi89 – 102Combined sources14
    Turni103 – 106Combined sources4
    Helixi110 – 123Combined sources14
    Beta strandi126 – 130Combined sources5
    Helixi136 – 144Combined sources9
    Beta strandi148 – 151Combined sources4
    Helixi157 – 168Combined sources12
    Helixi169 – 171Combined sources3
    Turni174 – 176Combined sources3
    Helixi180 – 191Combined sources12
    Helixi199 – 201Combined sources3
    Beta strandi205 – 209Combined sources5
    Helixi213 – 215Combined sources3
    Beta strandi223 – 225Combined sources3
    Helixi239 – 241Combined sources3
    Beta strandi249 – 253Combined sources5
    Turni268 – 271Combined sources4
    Helixi272 – 276Combined sources5
    Beta strandi278 – 284Combined sources7
    Turni288 – 291Combined sources4
    Beta strandi299 – 303Combined sources5
    Helixi309 – 312Combined sources4
    Helixi313 – 315Combined sources3
    Beta strandi317 – 321Combined sources5
    Helixi325 – 333Combined sources9
    Beta strandi338 – 340Combined sources3
    Helixi345 – 357Combined sources13
    Beta strandi359 – 362Combined sources4
    Turni365 – 367Combined sources3
    Helixi370 – 382Combined sources13
    Helixi384 – 398Combined sources15
    Helixi403 – 415Combined sources13

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2YJNX-ray3.09A1-421[»]
    ProteinModelPortaliA4F7P3.
    SMRiA4F7P3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 28 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG4108K6T. Bacteria.
    COG1819. LUCA.
    HOGENOMiHOG000253162.
    KOiK14368.
    OMAiSFRACAG.
    OrthoDBiPOG091H0BM4.

    Family and domain databases

    InterProiIPR010610. DUF1205.
    IPR030953. Glycosyl_450act.
    IPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 2 hits.
    PfamiPF06722. DUF1205. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR04516. glycosyl_450act. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A4F7P3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRVVFSSMAS KSHLFGLVPL AWAFRAAGHE VRVVASPALT EDITAAGLTA
    60 70 80 90 100
    VPVGTDVDLV DFMTHAGHDI IDYVRSLDFS ERDPATLTWE HLLGMQTVLT
    110 120 130 140 150
    PTFYALMSPD TLIEGMVSFC RKWRPDLVIW EPLTFAAPIA AAVTGTPHAR
    160 170 180 190 200
    LLWGPDITTR ARQNFLGLLP DQPEEHREDP LAEWLTWTLE KYGGPAFDEE
    210 220 230 240 250
    VVVGQWTIDP APAAIRLDTG LKTVGMRYVD YNGPSVVPEW LHDEPERRRV
    260 270 280 290 300
    CLTLGISSRE NSIGQVSIEE LLGAVGDVDA EIIATFDAQQ LEGVANIPDN
    310 320 330 340 350
    VRTVGFVPMH ALLPTCAATV HHGGPGSWHT AAIHGVPQVI LPDGWDTGVR
    360 370 380 390 400
    AQRTQEFGAG IALPVPELTP DQLRESVKRV LDDPAHRAGA ARMRDDMLAE
    410 420
    PSPAEVVGIC EELAAGRREP R
    Length:421
    Mass (Da):45,929
    Last modified:April 17, 2007 - v1
    Checksum:iE295A5A8CAF72BD1
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti179D → G in AAB84067 (PubMed:9353926).Curated1
    Sequence conflicti299D → H in AAB84067 (PubMed:9353926).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U77454 Genomic DNA. Translation: AAB84067.1.
    Y14332 Genomic DNA. Translation: CAA74710.1.
    AM420293 Genomic DNA. Translation: CAM00067.1.
    RefSeqiWP_009950402.1. NZ_ABFV01000100.1.

    Genome annotation databases

    EnsemblBacteriaiCAM00067; CAM00067; SACE_0726.
    KEGGisen:SACE_0726.
    PATRICi23408840. VBISacEry28377_0740.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U77454 Genomic DNA. Translation: AAB84067.1.
    Y14332 Genomic DNA. Translation: CAA74710.1.
    AM420293 Genomic DNA. Translation: CAM00067.1.
    RefSeqiWP_009950402.1. NZ_ABFV01000100.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2YJNX-ray3.09A1-421[»]
    ProteinModelPortaliA4F7P3.
    SMRiA4F7P3.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi405948.SeryN2_010100032633.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Proteomic databases

    PRIDEiA4F7P3.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAM00067; CAM00067; SACE_0726.
    KEGGisen:SACE_0726.
    PATRICi23408840. VBISacEry28377_0740.

    Phylogenomic databases

    eggNOGiENOG4108K6T. Bacteria.
    COG1819. LUCA.
    HOGENOMiHOG000253162.
    KOiK14368.
    OMAiSFRACAG.
    OrthoDBiPOG091H0BM4.

    Enzyme and pathway databases

    UniPathwayiUPA00240.
    BioCyciMetaCyc:MONOMER-17080.

    Family and domain databases

    InterProiIPR010610. DUF1205.
    IPR030953. Glycosyl_450act.
    IPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 2 hits.
    PfamiPF06722. DUF1205. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR04516. glycosyl_450act. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiERYC3_SACEN
    AccessioniPrimary (citable) accession number: A4F7P3
    Secondary accession number(s): O33935, O54224
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: April 17, 2007
    Last modified: November 2, 2016
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.