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Protein

3-alpha-mycarosylerythronolide B desosaminyl transferase

Gene

eryCIII

Organism
Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of alpha-L-mycarosylerythronolide B into erythromycin D in the erythromycin biosynthesis pathway.1 Publication

Catalytic activityi

dTDP-D-desosamine + 3-alpha-L-mycarosylerythronolide B = dTDP + erythromycin D.1 Publication

Kineticsi

kcat is 1 min(-1).

  1. KM=50 µM for 3-alpha-mycarosylerythronolide B1 Publication

    Pathwayi: erythromycin biosynthesis

    This protein is involved in the pathway erythromycin biosynthesis, which is part of Antibiotic biosynthesis.1 Publication
    View all proteins of this organism that are known to be involved in the pathway erythromycin biosynthesis and in Antibiotic biosynthesis.

    GO - Molecular functioni

    • transferase activity, transferring hexosyl groups Source: UniProtKB

    GO - Biological processi

    • antibiotic biosynthetic process Source: UniProtKB
    • metabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Antibiotic biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17080.
    UniPathwayiUPA00240.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-alpha-mycarosylerythronolide B desosaminyl transferase (EC:2.4.1.278)
    Alternative name(s):
    Desosaminyl transferase EryCIII
    Erythromycin biosynthesis protein CIII
    Gene namesi
    Name:eryCIII
    Ordered Locus Names:SACE_0726
    OrganismiSaccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
    Taxonomic identifieri405948 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeSaccharopolyspora
    Proteomesi
    • UP000006728 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence analysisAdd
    BLAST
    Chaini24 – 4213983-alpha-mycarosylerythronolide B desosaminyl transferasePRO_0000418493Add
    BLAST

    Proteomic databases

    PRIDEiA4F7P3.

    Interactioni

    Subunit structurei

    Heterotetramer composed of EryCII and EryCIII.1 Publication

    Protein-protein interaction databases

    STRINGi405948.SeryN2_010100032633.

    Structurei

    Secondary structure

    1
    421
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 65Combined sources
    Helixi11 – 144Combined sources
    Turni15 – 173Combined sources
    Helixi18 – 269Combined sources
    Beta strandi30 – 356Combined sources
    Helixi37 – 393Combined sources
    Helixi40 – 445Combined sources
    Turni45 – 473Combined sources
    Beta strandi50 – 523Combined sources
    Helixi59 – 657Combined sources
    Helixi68 – 747Combined sources
    Helixi84 – 874Combined sources
    Helixi89 – 10214Combined sources
    Turni103 – 1064Combined sources
    Helixi110 – 12314Combined sources
    Beta strandi126 – 1305Combined sources
    Helixi136 – 1449Combined sources
    Beta strandi148 – 1514Combined sources
    Helixi157 – 16812Combined sources
    Helixi169 – 1713Combined sources
    Turni174 – 1763Combined sources
    Helixi180 – 19112Combined sources
    Helixi199 – 2013Combined sources
    Beta strandi205 – 2095Combined sources
    Helixi213 – 2153Combined sources
    Beta strandi223 – 2253Combined sources
    Helixi239 – 2413Combined sources
    Beta strandi249 – 2535Combined sources
    Turni268 – 2714Combined sources
    Helixi272 – 2765Combined sources
    Beta strandi278 – 2847Combined sources
    Turni288 – 2914Combined sources
    Beta strandi299 – 3035Combined sources
    Helixi309 – 3124Combined sources
    Helixi313 – 3153Combined sources
    Beta strandi317 – 3215Combined sources
    Helixi325 – 3339Combined sources
    Beta strandi338 – 3403Combined sources
    Helixi345 – 35713Combined sources
    Beta strandi359 – 3624Combined sources
    Turni365 – 3673Combined sources
    Helixi370 – 38213Combined sources
    Helixi384 – 39815Combined sources
    Helixi403 – 41513Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2YJNX-ray3.09A1-421[»]
    ProteinModelPortaliA4F7P3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 28 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG4108K6T. Bacteria.
    COG1819. LUCA.
    HOGENOMiHOG000253162.
    KOiK14368.
    OMAiSFRACAG.
    OrthoDBiPOG091H0BM4.

    Family and domain databases

    InterProiIPR010610. DUF1205.
    IPR030953. Glycosyl_450act.
    IPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 2 hits.
    PfamiPF06722. DUF1205. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR04516. glycosyl_450act. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A4F7P3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRVVFSSMAS KSHLFGLVPL AWAFRAAGHE VRVVASPALT EDITAAGLTA
    60 70 80 90 100
    VPVGTDVDLV DFMTHAGHDI IDYVRSLDFS ERDPATLTWE HLLGMQTVLT
    110 120 130 140 150
    PTFYALMSPD TLIEGMVSFC RKWRPDLVIW EPLTFAAPIA AAVTGTPHAR
    160 170 180 190 200
    LLWGPDITTR ARQNFLGLLP DQPEEHREDP LAEWLTWTLE KYGGPAFDEE
    210 220 230 240 250
    VVVGQWTIDP APAAIRLDTG LKTVGMRYVD YNGPSVVPEW LHDEPERRRV
    260 270 280 290 300
    CLTLGISSRE NSIGQVSIEE LLGAVGDVDA EIIATFDAQQ LEGVANIPDN
    310 320 330 340 350
    VRTVGFVPMH ALLPTCAATV HHGGPGSWHT AAIHGVPQVI LPDGWDTGVR
    360 370 380 390 400
    AQRTQEFGAG IALPVPELTP DQLRESVKRV LDDPAHRAGA ARMRDDMLAE
    410 420
    PSPAEVVGIC EELAAGRREP R
    Length:421
    Mass (Da):45,929
    Last modified:April 17, 2007 - v1
    Checksum:iE295A5A8CAF72BD1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti179 – 1791D → G in AAB84067 (PubMed:9353926).Curated
    Sequence conflicti299 – 2991D → H in AAB84067 (PubMed:9353926).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U77454 Genomic DNA. Translation: AAB84067.1.
    Y14332 Genomic DNA. Translation: CAA74710.1.
    AM420293 Genomic DNA. Translation: CAM00067.1.
    RefSeqiWP_009950402.1. NZ_ABFV01000100.1.

    Genome annotation databases

    EnsemblBacteriaiCAM00067; CAM00067; SACE_0726.
    KEGGisen:SACE_0726.
    PATRICi23408840. VBISacEry28377_0740.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U77454 Genomic DNA. Translation: AAB84067.1.
    Y14332 Genomic DNA. Translation: CAA74710.1.
    AM420293 Genomic DNA. Translation: CAM00067.1.
    RefSeqiWP_009950402.1. NZ_ABFV01000100.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2YJNX-ray3.09A1-421[»]
    ProteinModelPortaliA4F7P3.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi405948.SeryN2_010100032633.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Proteomic databases

    PRIDEiA4F7P3.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAM00067; CAM00067; SACE_0726.
    KEGGisen:SACE_0726.
    PATRICi23408840. VBISacEry28377_0740.

    Phylogenomic databases

    eggNOGiENOG4108K6T. Bacteria.
    COG1819. LUCA.
    HOGENOMiHOG000253162.
    KOiK14368.
    OMAiSFRACAG.
    OrthoDBiPOG091H0BM4.

    Enzyme and pathway databases

    UniPathwayiUPA00240.
    BioCyciMetaCyc:MONOMER-17080.

    Family and domain databases

    InterProiIPR010610. DUF1205.
    IPR030953. Glycosyl_450act.
    IPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 2 hits.
    PfamiPF06722. DUF1205. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR04516. glycosyl_450act. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiERYC3_SACEN
    AccessioniPrimary (citable) accession number: A4F7P3
    Secondary accession number(s): O33935, O54224
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: April 17, 2007
    Last modified: September 7, 2016
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.