Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA sulfurtransferase

Gene

thiI

Organism
Reinekea blandensis MED297
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

Catalytic activityi

ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster.UniRule annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei324ATPUniRule annotation1
Binding sitei346ATP; via amide nitrogenUniRule annotation1
Binding sitei355ATPUniRule annotation1
Active sitei515Cysteine persulfide intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi242 – 243ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, TransferaseUniRule annotation, tRNA-bindingUniRule annotation
Biological processThiamine biosynthesisUniRule annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
ORF Names:MED297_12577Imported
OrganismiReinekea blandensis MED297Imported
Taxonomic identifieri314283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesSaccharospirillaceaeReinekea
Proteomesi
  • UP000005953 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi403 ↔ 515Redox-activeUniRule annotation

Keywords - PTMi

Disulfide bondUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi314283.MED297_12577.

Structurei

3D structure databases

ProteinModelPortaliA4BE87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini120 – 224THUMPInterPro annotationAdd BLAST105
Domaini463 – 542RhodaneseInterPro annotationAdd BLAST80

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation

Keywords - Domaini

Redox-active centerUniRule annotation

Phylogenomic databases

eggNOGiENOG4105D8I. Bacteria.
COG0301. LUCA.
COG0607. LUCA.
OrthoDBiPOG091H04VG.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI. 1 hit.
InterProiView protein in InterPro
IPR001763. Rhodanese-like_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR026340. Thiazole_biosynth_dom.
IPR020536. ThiI_AANH.
IPR004114. THUMP_dom.
IPR003720. tRNA_STrfase.
PfamiView protein in Pfam
PF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
SMARTiView protein in SMART
SM00981. THUMP. 1 hit.
SUPFAMiSSF52821. SSF52821. 1 hit.
TIGRFAMsiTIGR04271. ThiI_C_thiazole. 1 hit.
TIGR00342. TIGR00342. 1 hit.
PROSITEiView protein in PROSITE
PS50206. RHODANESE_3. 1 hit.
PS51165. THUMP. 1 hit.

Sequencei

Sequence statusi: Complete.

A4BE87-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHQLSALFW CVLIRQAFHG GTGRWESWLK DNLETGKPEH NMVVYSRAFL
60 70 80 90 100
EPALSRMKFI VKLFPEITIK TRPVRKLLVK QLTQNIRNVL LKISDDVRIR
110 120 130 140 150
NQWDAIEVWT RDGASDSVND DVYGALECIP GIAHVNRVRE FPLGDFDDIV
160 170 180 190 200
ENTREVWGEA LQGKTFVVRV KRTGQHDFTS VEAERYVGGG LNKLTDAKGV
210 220 230 240 250
DLHQPDVTIR LEIKHNRLFV IDRRRPGLGG FPLGSADSTL TLVSGGFDST
260 270 280 290 300
VAAYQMIRRG VKTHFLFFNL GGAAHENGVK EVIYYLWKKY GSSHKVQFVS
310 320 330 340 350
VPFEGVVENI LESVEDRYMG VVLKRMMMRA ANQVSDRLKL DSYVTGEALS
360 370 380 390 400
QVSSQTMMNQ RLIDDVADRL VIRPLAVTDK HDIVTIARQI GAASFVERIP
410 420 430 440 450
EYCGVISRKP NAACKRHDLE AAESAFDMTV LTAALEQAKV ESIDRMVWEP
460 470 480 490 500
TIDMEQALNQ SLDQAHAVII DVRHPHETES APFDAPGHEV IQLPFYSLNR
510 520 530 540
KFGELDQSRQ YLLYCDKGVM SKMHALHLAD AGFRNVGVYR QPQD
Length:544
Mass (Da):61,560
Last modified:April 3, 2007 - v1
Checksum:i0F7E4D49D71232F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAOE01000009 Genomic DNA. Translation: EAR09565.1.

Genome annotation databases

EnsemblBacteriaiEAR09565; EAR09565; MED297_12577.

Similar proteinsi

Entry informationi

Entry nameiA4BE87_9GAMM
AccessioniPrimary (citable) accession number: A4BE87
Entry historyiIntegrated into UniProtKB/TrEMBL: April 3, 2007
Last sequence update: April 3, 2007
Last modified: September 27, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteome, Reference proteomeImported