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A4AUE8 (A4AUE8_MARSH) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alanine--tRNA ligase HAMAP-Rule MF_00036
EC=6.1.1.7 HAMAP-Rule MF_00036
Alternative name(s):
Alanyl-tRNA synthetase HAMAP-Rule MF_00036
Gene names
Name:alaS HAMAP-Rule MF_00036
Ordered Locus Names:FB2170_08839 EMBL EAR00599.1
OrganismMaribacter sp. (strain HTCC2170 / KCCM 42371) [Complete proteome] [HAMAP]
Taxonomic identifier313603 [NCBI]
Taxonomic lineageBacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Maribacter

Protein attributes

Sequence length871 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP-Rule MF_00036

Catalytic activity

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP-Rule MF_00036 SAAS SAAS003156

Cofactor

Binds 1 zinc ion per subunit By similarity. SAAS SAAS003156 HAMAP-Rule MF_00036

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00036 SAAS SAAS023033.

Domain

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP-Rule MF_00036

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. HAMAP-Rule MF_00036

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding5631Zinc By similarity HAMAP-Rule MF_00036
Metal binding5671Zinc By similarity HAMAP-Rule MF_00036
Metal binding6651Zinc By similarity HAMAP-Rule MF_00036
Metal binding6691Zinc By similarity HAMAP-Rule MF_00036

Sequences

Sequence LengthMass (Da)Tools
A4AUE8 [UniParc].

Last modified April 3, 2007. Version 1.
Checksum: 8AF5B084F17FF992

FASTA87198,309
        10         20         30         40         50         60 
MNSKEIRAQF LDFFETKKHK IVPSAPMVIK DDPTLMFTNA GMNQFKEFFL GNSVAKSPRM 

        70         80         90        100        110        120 
ADTQKCLRVS GKHNDLEEVG KDTYHHTMFE MLGNWSFGDY FKKEAIEWAW ELLTDKLQID 

       130        140        150        160        170        180 
KDSLYVSVFE GSKDSDNLEM DNEAYELWSG IVPKDRIIMG NKKDNFWEMG DQGPCGPCSE 

       190        200        210        220        230        240 
IHVDIRPAEE KAETSGAFLV NQDHPLVVEI WNLVFMQYNR KADGALEPLP EKHVDTGMGF 

       250        260        270        280        290        300 
ERLCMVLQGV QSNYDTDVFT PIIREIETIT NADYGKNEEI DIAIRVIADH IRAVAFSIAD 

       310        320        330        340        350        360 
GQLPSNTGAG YVIRRILRRA IRYGFTFLNT KEPFMYRLVN VLTETMGNAF PELKDQRQLI 

       370        380        390        400        410        420 
ENVVKEEENS FLNTLEQGLV LLDNLVSKAK GKVVQGRKAF ELYDTYGFPI DLTALILEEK 

       430        440        450        460        470        480 
GYTLDEKGFH EAMKEQKDRS RSASEIAKED WMVLSDDSEQ EFVGYDLLET NVKLVKYRKV 

       490        500        510        520        530        540 
TSKKEGEQYQ MVFNLTPFYP EGGGQVGDKG YLEAPNGDVV YILDTKKENN EIVHFAKNLP 

       550        560        570        580        590        600 
KSTSDTFKAV VDKKQRQRTA ANHTATHLLH QALREVLGNH VEQKGSAVHS KYLRFDFSHF 

       610        620        630        640        650        660 
SKMTVEELRE VENFVNARIE GQLPLDENRS VPMEEALKGG AMALFGEEYG ETVRTIRFGQ 

       670        680        690        700        710        720 
SMELCGGAHV KITSDIWHFK IKSEGAVAAG IRRIEAITYD AVKDFYFHNN RSLFEIKDLL 

       730        740        750        760        770        780 
NNAQNPVNAV KALQDENAKL KKEVEQLLKD KAKNLKGDLL AELKEVNGIQ FLAKKVDLDA 

       790        800        810        820        830        840 
AGIKDLSFEM GNNKNNLFLL FGTEQNGKAL LSCYISKELV AEKNLNAGTI VRELGKCIQG 

       850        860        870 
GGGGQPFFAT AGGKNPAGIE EALSKAEEYL N

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References

[1]"Complete genome sequence of strain HTCC2170, a novel member of the genus Maribacter in the family Flavobacteriaceae."
Oh H.M., Kang I., Yang S.J., Jang Y., Vergin K.L., Giovannoni S.J., Cho J.C.
J. Bacteriol. 193:303-304(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HTCC2170 / KCCM 42371.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002157 Genomic DNA. Translation: EAR00599.1.
RefSeqYP_003862657.1. NC_014472.1.

3D structure databases

ProteinModelPortalA4AUE8.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEAR00599; EAR00599; FB2170_08839.
GeneID9719382.
KEGGfbc:FB2170_08839.
PATRIC42333579. VBIFlaBac16383_2044.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000156965.
KOK01872.
ProtClustDBPRK00252.

Enzyme and pathway databases

BioCycMSP313603:GH3X-2026-MONOMER.

Family and domain databases

HAMAPMF_00036_B. Ala_tRNA_synth_B.
InterProIPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. Pesterase_DHHA1.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PANTHERPTHR11777:SF6. PTHR11777:SF6. 1 hit.
PfamPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSPR00980. TRNASYNTHALA.
SMARTSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMSSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit.
SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMsTIGR00344. alaS. 1 hit.
PROSITEPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA4AUE8_MARSH
AccessionPrimary (citable) accession number: A4AUE8
Entry history
Integrated into UniProtKB/TrEMBL: April 3, 2007
Last sequence update: April 3, 2007
Last modified: May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info