A4AFX2 (A4AFX2_9ACTN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Mandelate racemase/muconate lactonizing enzyme EMBL EAR25878.1

Gene names

ORF Names:

A20C1_08358 EMBL EAR25878.1

Organism

marine actinobacterium PHSC20C1 EMBL EAR25878.1

Taxonomic identifier

312284 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › unclassified Actinobacteria › unclassified Actinobacteria (miscellaneous)

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Ontologies

Keywords
Ligand	Magnesium PDB 3NO1 Metal-binding SAAS SAAS013342 PDB 3NO1
Technical term	3D-structure PDB 3NO1 PDB 3MSY PDB 4H83
Gene Ontology (GO)
Molecular_function	catalytic activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	215	1	Magnesium PDB 3NO1
	Metal binding	241	1	Magnesium PDB 3NO1
	Metal binding	268	1	Magnesium PDB 3NO1

Sequences

	Sequence		Length	Mass (Da)
	A4AFX2 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 32F2FB2DF83FCE2C	FASTA	388	43,439
	10 20 30 40 50 60 MHDQRVHAER DPLDPQAHGL TITRIETIPM VAPLAREFRG SHYHMTHRAT IVTRVHTDAG 70 80 90 100 110 120 IIGEAYTGDE HETMFDIDRI IHEELAPTLI GQDAMAIERL WDSGYKVTFD ILRDRRLGLV 130 140 150 160 170 180 ALAAVNTAIW DAVGKALKMP LWKLWGGYRN ELPMIAIGGY YGEPLGSIAD EMHNYQELGL 190 200 210 220 230 240 AGVKFKVGGL SAAEDAARIT AAREAAGDDF IICIDANQGY KPAVAVDLSR RIADLNIRWF 250 260 270 280 290 300 EEPVEWHNDK RSMRDVRYQG SVPVCAGQTE FSASGCRDLM ETGAIDVCNF DSSWSGGPTA 310 320 330 340 350 360 WLRTAAIATS YDVQMGHHEE PQVSTHLLAS QPHGTIAECF HPDRDPFWWN MITNRPKLNN 370 380 GTLTLSDRPG LGWDLNWDYI DQYRVSKD

References

[1]	Murray A., Ferriera S., Johnson J., Kravitz S., Halpern A., Remington K., Beeson K., Tran B., Rogers Y.-H., Friedman R., Venter J.C. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: PHSC20C1 EMBL EAR25878.1.
[2]	"Crystal Structure of Mandelate racemase/muconate lactonizing enzyme from a Marine actinobacterium." Satyanarayana L., Burley S.K., Swaminathan S. Submitted (APR-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 20-388.
[3]	"Crystal Structure of Mandelate racemase/muconate lactonizing enzyme from a Marine actinobacterium in complex with magnesium." Satyanarayana L., Burley S.K., Swaminathan S. Submitted (JUN-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.16 ANGSTROMS) OF 2-388 IN COMPLEX WITH MAGNESIUM.
[4]	"Crystal structure of Mandelate racemase/muconate lactonizing enzyme." Kim J., Toro R., Bhosle R., Al Obaidi N.F., Morisco L.L., Wasserman S.R., Sojitra S., Washington E., Scott Glenn A., Chowdhury S., Evans B., Hammonds J., Stead M., Hillerich B., Love J., Seidel R.D., Imker H.J., Gerlt J.A., Almo S.C. Submitted (SEP-2012) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.09 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AAOB01000002 Genomic DNA. Translation: EAR25878.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3MSY	X-ray	2.50	A/B/C/D/E/F	20-388	[»]
3NO1	X-ray	2.16	A/B/C/D/E/F	2-388	[»]
4H83	X-ray	2.09	A/B/C/D/E/F	1-388	[»]

ProteinModelPortal

A4AFX2.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EAR25878; EAR25878; A20C1_08358.

PATRIC

25922165. VBIMarAct105506_0948.

Family and domain databases

InterPro

IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N.
IPR001354. MR_MLE.
[Graphical view]

PANTHER

PTHR13794. PTHR13794. 1 hit.

Pfam

PF01188. MR_MLE. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]

SMART

SM00922. MR_MLE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

A4AFX2.

Entry information

Entry name

A4AFX2_9ACTN

Accession

Primary (citable) accession number: A4AFX2

Entry history

Integrated into UniProtKB/TrEMBL:	April 3, 2007
Last sequence update:	April 3, 2007
Last modified:	April 3, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)