Reviewed, UniProtKB/Swiss-Prot A3RM23 (L_RABVI)

Names and origin

Protein names

Recommended name:
    Large structural protein
      Short name=Protein L
Alternative name(s):
    Transcriptase
    Replicase
Including the following 3 domains:
    1- Recommended name:
            RNA-directed RNA polymerase
              EC=2.7.7.48
    2- Recommended name:
            mRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.56
    3- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.-

Gene names

Name:

L

Organism

Rabies virus (strain India) (RABV) [Complete proteome]

Taxonomic identifier

445790 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Lyssavirus

Virus host

Mammalia [TaxID: 40674]

Protein attributes

Sequence length	2127 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-7-N-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
Subunit structure	Interacts with the P protein to form the functional polymerase By similarity.
Subcellular location	Virion. CytoplasmBy similarity.
Sequence similarities	Belongs to the rhabdoviruses protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW virion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2127	2127	Large structural protein		PRO_0000294422
Regions
	Domain	611 – 799	189	RdRp catalytic
	Region	1562 – 2127	566	Interaction with P protein By similarity
Natural variations
	Natural variant	93	1	T → A in strain: Isolate Germany.
	Natural variant	232	1	H → Q in strain: Isolate Germany.
	Natural variant	361	1	L → V in strain: Isolate Germany.
	Natural variant	471	1	P → S in strain: Isolate Germany.
	Natural variant	488	1	A → T in strain: Isolate Germany.
	Natural variant	502	1	A → V in strain: Isolate Germany.
	Natural variant	525	1	T → S in strain: Isolate Germany.
	Natural variant	966	1	T → A in strain: Isolate Germany.
	Natural variant	1373	1	G → D in strain: Isolate Germany.
	Natural variant	1825	1	Y → C in strain: Isolate Germany.
	Natural variant	1841	1	K → N in strain: Isolate Germany.
	Natural variant	1845	1	H → Q in strain: Isolate Germany.
	Natural variant	1872	1	F → C in strain: Isolate Germany.
	Natural variant	1904	1	R → K in strain: Isolate Germany.
	Natural variant	1960	1	R → G in strain: Isolate Germany.
	Natural variant	2091	1	S → N in strain: Isolate Germany.

Sequences

	Sequence		Length	Mass (Da)
	A3RM23-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 55202B0E620305C8	FASTA	2,127	242,770
	10 20 30 40 50 60 MLDPGEVYDD PVDPIESDAE PRGAPTVPNI LRNSDYNLNS PLIEDPARLM LEWLTTGNRP 70 80 90 100 110 120 YRMTLTDNCS RSYKVLKDYF KKVDLGSLKV GGTAAQSMIS LWLYGAHSES NRSRKCITEL 130 140 150 160 170 180 AHFYSKSSPI EKLLNCTLGN RGLRIPPEGV LSCLERVDYD KAFGRYLANT YSSYLFFHVI 190 200 210 220 230 240 TLYMNALDWD EEKTILALWK ELTSVDIGKD LVKFKDQIWG LLIVTKDFVY SHSSNCLFDR 250 260 270 280 290 300 NYTLMLKDLF LSRFNSLMIL LSPPEPRYSD DLISQLCQLY IAGDQVLSMC GNSGYEVIKI 310 320 330 340 350 360 LEPYVVNSLV QRAERFRPLI HSLGDFPVFI KDKVSQLEGT FGPSAKRFFG VLDQFDNIHD 370 380 390 400 410 420 LVFVYGCYRH WGHPYIDYRK GLSKLYDQVH IKKVIDKSYQ ECLASDLARR ILRWGFDKYS 430 440 450 460 470 480 KWYLDSRLLT RDHPLTPYIK TQTWPPKHIV DLVGDTWHKL PITQIFEIPE PMDPSEILDD 490 500 510 520 530 540 KSHSFTRARL ASWLSENRGG PAPSEKVIIT ALSKPPVNPR EFLKTIDLGG LPDEDLIIGL 550 560 570 580 590 600 KPKERELKIE GRFFALMSWN LRLYFVITEK LLANYILPLF DALTMTDNLN KVFKKLIDRV 610 620 630 640 650 660 TGQGLLDYSR VTYAFHLDYE KWNNHQRLES TEDVFSVLDH VFGLKRVFSR THEFFQKSWI 670 680 690 700 710 720 YYSDRSDLIG LWEDQIYCLD MSNGPTCWNG QDGGLEGLRQ KGWSLVSLLM IDRESQTRNT 730 740 750 760 770 780 RTKILAQGDN QVLCPTYMLS PGLSREGLLY ELESISRNAL SIYRAIEEGA SKLGLIIKKE 790 800 810 820 830 840 ETMCSYDFLI YGKTPLFRGN ILVPESKRWA RVSCISNDQI VNLANIMSTV STNALTVAQH 850 860 870 880 890 900 SQSLIKPMRD FLLMSVQAVF HYLLFSPILK GRVYKILSAE GESFLLAMSR IIYLDPSLGG 910 920 930 940 950 960 VSGMSLGRFH IRQFSDPVSE GLSFWREIWL SSHESWIHAL CQEAGNPDLG ERTLESFTRL 970 980 990 1000 1010 1020 LEDPTTLNIK GGASPTILLK DAIRKALYDE VDKVENSEFR EAILLSKTHR DNFILFLKSV 1030 1040 1050 1060 1070 1080 EPLFPRFLSE LFSSSFLGIP ESIIGLIQNS RTIRRQFRRS LSRTLEESFY NSEIHGINRM 1090 1100 1110 1120 1130 1140 TQTPQRVGRV WPCSSERADL LREISWGRKV VGTTVPHPSE MLGLLPKSSI SCPCGATGGG 1150 1160 1170 1180 1190 1200 NPRVSVSVLP SFDQSFFSRG PLKGYLGSST SMSTQLFHAW EKVTNVHVVK RALSLKESIN 1210 1220 1230 1240 1250 1260 WFITRNSNLA QTLIRNIMSL TGPDFPLEEA PVFKRTGSAL HRFKSARYSE GGYSSVCPNL 1270 1280 1290 1300 1310 1320 LSHISVSTDT MSDLTQDGKN YDFMFQPLML YAQTWTSELV QKDTRLRDST FHWHLRCNRC 1330 1340 1350 1360 1370 1380 VRPIDDITLE TSQIFEFPDV SKRISRMVSG AVPHFQKLPD IRLKPGDFES LSGREKSRHI 1390 1400 1410 1420 1430 1440 GSAQGLLYSI LVAIHDSGYN DGTIFPVNIY GKVSPRDYLR GLARGVLIGS SICFLTRMTN 1450 1460 1470 1480 1490 1500 ININRPLELI SGVISYILLR LDNHPSLYIM LREPSLRGEI FSIPQKIPAA YPTTMKEGNR 1510 1520 1530 1540 1550 1560 SILCYLQHVL RYEREVITAS PENDWLWIFS DFRSAKMTYL TLITYQSHLL LQRVERNLSK 1570 1580 1590 1600 1610 1620 SMRANLRQMS SLMRQVLGGH GEDTLESDDD VQRLLKDSLR RTRWVDQEVR HAARTMTGDY 1630 1640 1650 1660 1670 1680 SPNKKLSRKA GGSEWVCSAQ QVAVSTSANP APVLELDIRA LSKRFQNPLI SGLRVVQWAT 1690 1700 1710 1720 1730 1740 GAHYKLKPIL DDLNVFPSLC LVVGDGSGGI SRAVLNMFPD AKLVFNSLLE VNDLMASGTH 1750 1760 1770 1780 1790 1800 PLPPSAIMSG GDDIVSRVID FDSIWEKPSD LRNLTTWKYF QSVQKQVNMS YDLIICDAEV 1810 1820 1830 1840 1850 1860 TDIASINRIT LLMSDFALSI DGPLYLVFKT YGTMLVNPDY KAIQHLSRAF PSVTGFITQV 1870 1880 1890 1900 1910 1920 TSSFSSELYL RFSKRGKFFR DAEYLTSSTL REMSLVLFNC SSPRSEMQRA RSLNYQDLVR 1930 1940 1950 1960 1970 1980 GFPEEIISNP YNEMIITLID SDVESFLVHK MVDDLELQRR TLSKVAIIIA IMIVFSNRVF 1990 2000 2010 2020 2030 2040 NVSKPLTDPL FYPPSDPKIL RHFNICCSTM MYLSTALGDV PSFARLHDLY NRPITYYFRK 2050 2060 2070 2080 2090 2100 QVIRGNIYLS WSWSDDTAVF KRVACNSSLS LSSHWIRLIY KIVKTTRLVG SIEDLSGEIE 2110 2120 RHLRGYNRWI TLEDIRCRSS LLDYSCL

References

[1]	"Virological characterization of cases of transplantation-associated Rabies in Germany." Pfefferle S., Panning M., Drosten C. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate Germany.
[2]	"Complete nucleotide sequencing of an Indian isolate of Rabies virus." Desai A., Nagaraja T., Muhamuda K., Madhusudana S., Ravi V. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY956319 Genomic RNA. Translation: AAX56085.1. EF437215 Genomic RNA. Translation: ABO15580.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR017234. RNA-dir_RNA_pol_rhabdovir. IPR014023. RNA_pol_cat. IPR001016. RNA_pol_L_viral. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF037546. RNA_pol_RhabdoV_sub. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_RABVI

Accession

Primary (citable) accession number: A3RM23
Secondary accession number(s): Q58FH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	April 3, 2007
Last modified:	November 25, 2008
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)