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Protein

Hyaluronidase-2

Gene
N/A
Organism
Bitis arietans (African puff adder)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Snake venom endo-hyaluronidase that degrades hyaluronan to smaller oligosaccharide fragments. In venom, it is not toxic by itself, but increases the diffusion of other venom proteins by degrading the extracellular matrix. In addition, it displays antiedematogenic activity (By similarity).By similarity

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei135 – 1351Proton donorBy similarity

GO - Molecular functioni

  1. hyalurononglucosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase-2 (EC:3.2.1.35)
Short name:
Hy-2
Alternative name(s):
Hyaluronoglucosaminidase-2
Venom spreading factor
OrganismiBitis arietans (African puff adder)
Taxonomic identifieri8692 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeViperinaeBitis

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323By similarityAdd
BLAST
Chaini24 – 449426Hyaluronidase-2PRO_0000420462Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi47 ↔ 340By similarity
Glycosylationi67 – 671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi111 – 1111N-linked (GlcNAc...)Sequence Analysis
Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi211 ↔ 227By similarity
Glycosylationi357 – 3571N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi365 ↔ 376By similarity
Disulfide bondi370 ↔ 427By similarity
Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi429 ↔ 438By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA3QVP0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini427 – 43812EGF-likeAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Curated
Contains 1 EGF-like domain.Curated

Keywords - Domaini

EGF-like domain, Signal

Phylogenomic databases

HOVERGENiHBG052053.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000742. EG-like_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SMARTiSM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A3QVP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYHLWIKCLA AWIFLKRCNG VHAMPAKAPM YPNEPFIVLW NAPTTQCPLR
60 70 80 90 100
YKVDLDLKTF HIVANANDSL SGSVVAIFYP NHLGVYPHID ERGHFFHGII
110 120 130 140 150
PQNESLTKHL NKSKSDINRM IPLKTFHGLG VIDWENWRPQ WDRNWGSKNV
160 170 180 190 200
YRNRSIQFAK KLHPELSEDK IKRLAKKEYE KAAKSFMRDT LLLAEEMRPN
210 220 230 240 250
GYWGYYLYPD CQNYDYKTKG DQYTGKCPDI EMSRNDQLLW LWRDSTALFP
260 270 280 290 300
NVYLEIILRS SDNALKFVHH RLKESMRIAS MAREDYALPV FVYARPFYAY
310 320 330 340 350
TFEPLTQEDL VTTVGETAAM GAAGIVFWGS MQYASTVDSC QKVKTYMNGP
360 370 380 390 400
LGRYIVNVTT AAKICSHALC RKNGRCVRKH SDSNAFLHLF PESFRIMVHA
410 420 430 440
NATEKKAIVK GKLELKDLIY LRKNFMCQCY QGWKGLYCEE YSIKDIRKI
Length:449
Mass (Da):52,255
Last modified:April 3, 2007 - v1
Checksum:i6F6917A151EE7706
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ840257 mRNA. Translation: ABI33945.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ840257 mRNA. Translation: ABI33945.1.

3D structure databases

ProteinModelPortaliA3QVP0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG052053.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000742. EG-like_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SMARTiSM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of cDNAs encoding viper venom hyaluronidases: cross-generic sequence conservation of full-length and unusually short variant transcripts."
    Harrison R.A., Ibison F., Wilbraham D., Wagstaff S.C.
    Gene 392:22-33(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Venom gland.

Entry informationi

Entry nameiHYAL2_BITAR
AccessioniPrimary (citable) accession number: A3QVP0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: April 3, 2007
Last modified: January 7, 2015
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.