A3QVN2 (HYAL_ECHOC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Hyaluronidase Short name=Hy EC=3.2.1.35 Alternative name(s): Hyaluronoglucosaminidase Venom spreading factor
Organism	Echis ocellatus (Ocellated saw-scaled viper)
Taxonomic identifier	99586 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Viperidae › Viperinae › Echis

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Snake venom endo-hyaluronidase that degrades hyaluronan to smaller oligosaccharide fragments. In venom, it is not toxic by itself, but increases the diffusion of other venom proteins by degrading the extracellular matrix. In addition, it displays antiedematogenic activity By similarity.
Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	EGF-like domain Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hyalurononglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	By similarity
	Chain	24 – 449	426	Hyaluronidase		PRO_0000420456
Regions
	Domain	427 – 438	12	EGF-like
Sites
	Active site	135	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	67	1	N-linked (GlcNAc...) Potential
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Glycosylation	153	1	N-linked (GlcNAc...) Potential
	Glycosylation	357	1	N-linked (GlcNAc...) Potential
	Glycosylation	401	1	N-linked (GlcNAc...) Potential
	Disulfide bond	47 ↔ 340		By similarity
	Disulfide bond	211 ↔ 227		By similarity
	Disulfide bond	365 ↔ 376		By similarity
	Disulfide bond	370 ↔ 427		By similarity
	Disulfide bond	429 ↔ 438		By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3QVN2 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 4AA76609010C0660	FASTA	449	52,543
	10 20 30 40 50 60 MYHLWIKCLA AWIFLKRFNG VHVMHAKAPM YPNEPFLVFW NAPTTQCRLR YKVDLDLKTF 70 80 90 100 110 120 HIVANANDTL SGSAVTIFYP THLGIYPHID DRGHFFHGII PQNESLTKHL DKSKSDINRI 130 140 150 160 170 180 IPLKTFHGLG VIDWENWRPQ WDRNWGNKNV YRNRSIQFAR DLHPELSENK IRRLAKAEYE 190 200 210 220 230 240 KAAKSFMRDT LLLAEEMRPD GYWGYYLYPD CQNYDYKTKG DQYTGKCPDI EMSRNDQLLW 250 260 270 280 290 300 LWRESTALFP NVYLEIILRS SDNALKFVHH RLKESMRIAS MAREDYALPV FVYARPFYAY 310 320 330 340 350 360 TFEPLTQEDL VTTVGETAAM GAAGIVFWGS MQYASTVDSC QKVKKYMNGP LGRYIINVTT 370 380 390 400 410 420 AAKICSHALC RKNGRCVRKH SDSNAFLHLF PESFRIMVHA NATEKKVIVK GKLELENLIY 430 440 LRENFMCQCY QGWQGLYCEE YSIKDIRKI

References

[1]

"Identification of cDNAs encoding viper venom hyaluronidases: cross-generic sequence conservation of full-length and unusually short variant transcripts." Harrison R.A., Ibison F., Wilbraham D., Wagstaff S.C. Gene 392:22-33(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Venom gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ840249 mRNA. Translation: ABI33937.1.
3D structure databases
ProteinModelPortal	A3QVN2.
ModBase	Search...
Protein family/group databases
CAZy	GH56. Glycoside Hydrolase Family 56.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG052053.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR000742. EG-like_dom. IPR017853. Glycoside_hydrolase_SF. IPR018155. Hyaluronidase. [Graphical view]
PANTHER	PTHR11769. PTHR11769. 1 hit.
Pfam	PF01630. Glyco_hydro_56. 1 hit. [Graphical view]
PIRSF	PIRSF038193. Hyaluronidase. 1 hit.
PRINTS	PR00846. GLHYDRLASE56.
SMART	SM00181. EGF. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYAL_ECHOC

Accession

Primary (citable) accession number: A3QVN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2012
Last sequence update:	April 3, 2007
Last modified:	March 6, 2013
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program