Reviewed, UniProtKB/Swiss-Prot A3QK15 (AACS_DANRE)

Names and origin

Protein names

Recommended name:
    Acetoacetyl-CoA synthetase
    EC=6.2.1.16

Gene names

Name:	aacs
ORF Names:	si:dkey-215k6.2, zgc:56105

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	673 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Activates acetoacetate to acetoacetyl-CoA By similarity.
Catalytic activity	ATP + acetoacetate + CoA = AMP + diphosphate + acetoacetyl-CoA.
Subcellular location	Cytoplasm › cytosol By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetoacetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A3QK15-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: A3QK15-2)

The sequence of this isoform differs from the canonical sequence as follows:
     346-359: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 673	673	Acetoacetyl-CoA synthetase		PRO_0000315789
Natural variations
	Alternative sequence	346 – 359	14	Missing in isoform 2.		VSP_030703
Experimental info
	Sequence conflict	164	1	V → I in AAH50501. Ref.2
	Sequence conflict	431	1	V → M in AAH50501. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 71CACCD9B2049782	FASTA	673	74,967
	10 20 30 40 50 60 MSKDTEAKSE EIMESKVLWY PDSKRNTQTD RFRTLVNREF GLNLANYNDL YQWSVDSYPE 70 80 90 100 110 120 FWAQVWKFCG ITCSKMYEEV VDVSKRISDV PEWFKGSRLN YAENLLKHKD QDKVALYAAS 130 140 150 160 170 180 EAKEEIVKVT FGELRRDVAL FAAAMRKMGI KIGDRVVGYL PNGVHAVEAM LAAASIGAIW 190 200 210 220 230 240 SSTSPDFGVN GVLDRISQIQ PKLIFSVAAV VYNGKQHDHM EKLQNVVKGL PDLKKVVVIP 250 260 270 280 290 300 YVRSRQETDL SKIPNSVFLE DFLATGKEGD QDPQLEFEQL PFSHPLFIMY SSGTTGAPKC 310 320 330 340 350 360 MVHSAGGTLI QHLKEHILHG NMTFNDVIIY YTTTGWMMWN WLISSLAVGA SVVLYDGSPL 370 380 390 400 410 420 VPSANVLWDL VDRLGITIFG TGAKWLAVLE ERDQKPASTH SLQTLHTLLS TGSPLKPQSY 430 440 450 460 470 480 EYVYSCIKNN VLLGSISGGT DIISCFMGQN MTVPVYRGEI QARNLGMAVE SWSCEGKPVW 490 500 510 520 530 540 GESGELVCLK PIPCQPTHFW NDENGSKYHK AYFSTFPGVW AHGDYCKINP KTGGVVMLGR 550 560 570 580 590 600 SDGTLNPNGV RFGSSEIYNI VEAFDEVSDS LCVPQYNSDG EERVILFLKM GPNKSFSQEL 610 620 630 640 650 660 VGKIRGAIRV ALSARHVPAL ILETKDIPYT ISGKKVEVAV KQVIAGKEVT QRGAFSNPDS 670 LDLYKNLPEL QNF
	Isoform 2. Checksum: E648C2C4A3F391D0	FASTA	659	73,637
	10 20 30 40 50 60 MSKDTEAKSE EIMESKVLWY PDSKRNTQTD RFRTLVNREF GLNLANYNDL YQWSVDSYPE 70 80 90 100 110 120 FWAQVWKFCG ITCSKMYEEV VDVSKRISDV PEWFKGSRLN YAENLLKHKD QDKVALYAAS 130 140 150 160 170 180 EAKEEIVKVT FGELRRDVAL FAAAMRKMGI KIGDRVVGYL PNGVHAVEAM LAAASIGAIW 190 200 210 220 230 240 SSTSPDFGVN GVLDRISQIQ PKLIFSVAAV VYNGKQHDHM EKLQNVVKGL PDLKKVVVIP 250 260 270 280 290 300 YVRSRQETDL SKIPNSVFLE DFLATGKEGD QDPQLEFEQL PFSHPLFIMY SSGTTGAPKC 310 320 330 340 350 360 MVHSAGGTLI QHLKEHILHG NMTFNDVIIY YTTTGWMMWN WLISSLVPSA NVLWDLVDRL 370 380 390 400 410 420 GITIFGTGAK WLAVLEERDQ KPASTHSLQT LHTLLSTGSP LKPQSYEYVY SCIKNNVLLG 430 440 450 460 470 480 SISGGTDIIS CFMGQNMTVP VYRGEIQARN LGMAVESWSC EGKPVWGESG ELVCLKPIPC 490 500 510 520 530 540 QPTHFWNDEN GSKYHKAYFS TFPGVWAHGD YCKINPKTGG VVMLGRSDGT LNPNGVRFGS 550 560 570 580 590 600 SEIYNIVEAF DEVSDSLCVP QYNSDGEERV ILFLKMGPNK SFSQELVGKI RGAIRVALSA 610 620 630 640 650 RHVPALILET KDIPYTISGK KVEVAVKQVI AGKEVTQRGA FSNPDSLDLY KNLPELQNF

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: SJD.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR759959 Genomic DNA. Translation: CAM56650.1. BC050501 mRNA. Translation: AAH50501.1.
IPI	IPI00490036. IPI00861322.
RefSeq	NP_957303.1.
UniGene	Dr.25204
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3QK15.
Genome annotation databases
Ensembl	ENSDART00000010101; ENSDARP00000002180; ENSDARG00000012468; Danio rerio. [Genome view]
GeneID	393984.
KEGG	dre:393984.
Organism-specific databases
CTD	393984.
ZFIN	ZDB-GENE-040426-903. aacs.
Phylogenomic databases
OMA	WAHGDYA.
Enzyme and pathway databases
BRENDA	6.2.1.16. 96826.
Gene expression databases
ArrayExpress	A3QK15.
Bgee	A3QK15.
Family and domain databases
InterPro	IPR005914. Acac_CoA_synth. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01217. ac_ac_CoA_syn. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AACS_DANRE

Accession

Primary (citable) accession number: A3QK15
Secondary accession number(s): Q7ZU87

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project