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Protein

Atypical kinase COQ8B, mitochondrial

Gene

coq8b

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Atypical kinase involved in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration. Its substrate specificity is unclear: does not show any protein kinase activity. Probably acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway. Required for podocyte migration.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei313ATPBy similarity1
Binding sitei331ATPBy similarity1
Active sitei461Proton acceptorBy similarity1
Binding sitei466ATPBy similarity1
Binding sitei480ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi418 – 421ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • cerebellar Purkinje cell layer morphogenesis Source: UniProtKB
  • glomerular basement membrane development Source: ZFIN
  • glomerular visceral epithelial cell development Source: ZFIN
  • ubiquinone biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Atypical kinase COQ8B, mitochondrialCurated (EC:2.7.-.-By similarity)
Alternative name(s):
AarF domain-containing protein kinase 4By similarity
Coenzyme Q protein 8BBy similarity
Gene namesi
Name:coq8bBy similarity
Synonyms:adck4By similarity
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 15

Organism-specific databases

ZFINiZDB-GENE-060503-803. adck4.

Subcellular locationi

  • Mitochondrion membrane By similarity; Single-pass membrane protein By similarity
  • Cytoplasmcytosol By similarity
  • Cell membrane By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei189 – 205HelicalSequence analysisAdd BLAST17

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Morpholino knockdown of the protein causes nephrosis phenotype of periorbital edema and total body edema in 54% of embryos at 120 hpf. The glomerular structures are altered, with podocyte foot process effacement and disorganization, rarefaction of slit membranes, and disorganization of the glomerular basement membranes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004255721 – 624Atypical kinase COQ8B, mitochondrialAdd BLAST624

Proteomic databases

PaxDbiA3QJU3.
PeptideAtlasiA3QJU3.

Interactioni

Subunit structurei

Homodimer; homodimerizes via its transmembrane region. Interacts with the multi-subunit COQ enzyme complex.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000013965.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini285 – 517Protein kinaseAdd BLAST233

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi249 – 252KxGQ motifBy similarity4
Motifi310 – 313AAAS motifBy similarity4

Domaini

Adopts an atypical protein kinase-like fold: while it adopts a core fold similar to that of well-characterized protein kinase-like domains. The KxGQ motif completely occludes the typical substrate binding pocket. Nucleotide-binding opens the substrate binding pocket and flips the active site from inside the hydrophobic core into a catalytically competent, solvent-exposed posture.By similarity

Sequence similaritiesi

Contains 1 protein kinase domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1234. Eukaryota.
COG0661. LUCA.
GeneTreeiENSGT00550000074739.
HOGENOMiHOG000201140.
HOVERGENiHBG061318.
KOiK08869.
OMAiPCGGSWA.
OrthoDBiEOG091G02P9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR004147. UbiB_dom.
[Graphical view]
PfamiPF03109. ABC1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.

Sequencei

Sequence statusi: Complete.

A3QJU3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLSEVLQVL RGAGKVGAAF TSTQGEQLRL MACNSTFGAG MKAAAEAVEG
60 70 80 90 100
VMGTVMGGGD MTSKTDEFAG IEKWEEMDLD EAAKWSVASE MPPDFSSKDG
110 120 130 140 150
RGETSETPVG AATGTIKGAG WPAQNTRFLH VSASQHHFRF VHDSIVARLS
160 170 180 190 200
PEDIQRAREA KQNIARPVRQ KLNERAKERK VPATRISRLA NFGGLAVGLG
210 220 230 240 250
IGAIAEVAKQ SFGGKRSEVG ALLDSPLLSE ANAERIVNTL CKVRGAALKI
260 270 280 290 300
GQMLSIQDNS FINPQLQKIF ERVRQSADFM PAWQMHKVLE EELGSGWREK
310 320 330 340 350
LSSIEEKPFA AASIGQVHHG VLPGGKEIAM KIQYPGVAES IHSDINNLMS
360 370 380 390 400
VLKMSVVLPD GLFADSSLEV LQRELAWECD YEREAKCAKR FRNLLKGDPV
410 420 430 440 450
FVVPEVFDEL SARRVITMEL VNGVPLDRCV DLDQETRNEI CFNILQLCLR
460 470 480 490 500
ELFEFRFMQT DPNWSNFFYN SEQNKIFLLD FGACRDYPEL FTDHYIEVVH
510 520 530 540 550
AASVGDRATV LKKSKDLKFL TGFEAKAFED AHVEAVMILG EAFASAEAFD
560 570 580 590 600
FGTQSTTQRI QSLIPVMLRH RLTPPPEESY SLHRKMAGSF LICSKLKARF
610 620
SCRNMFLDVY NAYKRQQQER RSQV
Length:624
Mass (Da):69,460
Last modified:November 13, 2013 - v2
Checksum:i4E42F359888C155F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR318657 Genomic DNA. No translation available.
RefSeqiXP_001336310.1. XM_001336274.7.

Genome annotation databases

EnsembliENSDART00000172195; ENSDARP00000132291; ENSDARG00000101130.
GeneIDi799071.
KEGGidre:799071.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR318657 Genomic DNA. No translation available.
RefSeqiXP_001336310.1. XM_001336274.7.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000013965.

Proteomic databases

PaxDbiA3QJU3.
PeptideAtlasiA3QJU3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000172195; ENSDARP00000132291; ENSDARG00000101130.
GeneIDi799071.
KEGGidre:799071.

Organism-specific databases

CTDi308453.
ZFINiZDB-GENE-060503-803. adck4.

Phylogenomic databases

eggNOGiKOG1234. Eukaryota.
COG0661. LUCA.
GeneTreeiENSGT00550000074739.
HOGENOMiHOG000201140.
HOVERGENiHBG061318.
KOiK08869.
OMAiPCGGSWA.
OrthoDBiEOG091G02P9.

Miscellaneous databases

PROiA3QJU3.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR004147. UbiB_dom.
[Graphical view]
PfamiPF03109. ABC1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCOQ8B_DANRE
AccessioniPrimary (citable) accession number: A3QJU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: November 13, 2013
Last modified: November 30, 2016
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.