A3QJF9 (RLMF_SHELP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase F
EC=2.1.1.181

Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	Shew_3741

Organism

Shewanella loihica (strain ATCC BAA-1088 / PV-4) [Complete proteome] [HAMAP]

Taxonomic identifier

323850 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848
Catalytic activity	S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848
Subcellular location	Cytoplasm Potential HAMAP MF_01848.
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349957

Sequences

	Sequence		Length	Mass (Da)
	A3QJF9 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: 406EDFE8AA071260	FASTA	398	43,977
	10 20 30 40 50 60 MTPSRKPARP GAKKVVSQEP SAKASRPKPS SQSKSKAQPK AKPESKPQAK SQAKPQAKSK 70 80 90 100 110 120 PVEGKSQGAS KGLHPRNLHG QGYDFDALMD TTPELKPFVR PNPYGNLSID FADPRAVKLL 130 140 150 160 170 180 NLALLRLHYG LSYWDIPEGF LCPPIPGRVD YLHYLADLLA ESTPASKGGK RKLPNVTRAP 190 200 210 220 230 240 KGERVVALDI GTGANGIYPL LGHQVYGWRF VASDIDPVSL KNVERIIANN PQLAGKLSLR 250 260 270 280 290 300 LQSDSKQIFT GIIRAEDRFD LTLCNPPFHA SLAEASRGSE RKLKNLAANK RAKGHEVAKS 310 320 330 340 350 360 KEKLNFGGQK AELWCEGGEQ AFLGQMIRES QKFGSQCLWF TSLVSKKENL KPCYRLLEQV 370 380 390 GAKRVETLEM AQGNKLTRVL AWTFLTPEQH SLWGLYRV

References

[1]

"Complete sequence of Shewanella loihica PV-4." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Romine M.F., Serres G., Fredrickson J., Tiedje J., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1088 / PV-4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000606 Genomic DNA. Translation: ABO25607.1.
RefSeq	YP_001095866.1. NC_009092.1.
3D structure databases
ProteinModelPortal	A3QJF9.
ModBase	Search...
Protein-protein interaction databases
STRING	A3QJF9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4920916.
GenomeReviews	Gene locus Shew_3741 in contig CP000606_GR.
KEGG	slo:Shew_3741.
NMPDR	fig|323850.3.peg.1675.
PATRIC	23519438. VBISheLoi132094_3742.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3129.
HOGENOM	HBG747880.
OMA	WSFVGSD.
PhylomeDB	A3QJF9.
ProtClustDB	PRK11727.
Enzyme and pathway databases
BioCyc	SLOI323850:SHEW_3741-MONOMER.
Family and domain databases
HAMAP	MF_01848. 23SrRNA_methyltr_F. [Tree]
InterPro	IPR016909. rRNA_lsu_methyltransferase_F. IPR010286. SAM-Mtase_prd. [Graphical view]
KO	K06970.
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_SHELP

Accession

Primary (citable) accession number: A3QJF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	April 17, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program