A3QIU8 (PLSB_SHELP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	Shew_3530

Organism

Shewanella loihica (strain ATCC BAA-1088 / PV-4) [Complete proteome] [HAMAP]

Taxonomic identifier

323850 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	807 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 807	807	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049458
Regions
	Motif	308 – 313	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	A3QIU8 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: DD866D7D63DCD02B	FASTA	807	91,491
	10 20 30 40 50 60 MSKQDSLWFK SLRWIQKKLV HTIVVPQDPF ADLNLDSDRP LVYVMKTESI SDIAALSEVT 70 80 90 100 110 120 DRLGLPSPYQ PLDVEGEQAP RVVCVEGAKP LLGEREGNEF YLESFSRLLS AHKKHPELDI 130 140 150 160 170 180 QLVPVSLYWG RTPGKEDDSM KAAVLERESP TWLRKCLMII FLGRHNFVQF SNAMSLRYMA 190 200 210 220 230 240 DEHGTDKRIA QKLARVARVH FSRQRKVMTG PVLPNRQNLF HALLKSESMK KAILEEAQSK 250 260 270 280 290 300 KISEAKARET AMEYLDEIAA DYSDSLVRIA ERFLTWLWNK LYKGINIKGA EQIRQLHHDG 310 320 330 340 350 360 HEIVYVPCHR SHMDYLLLSY ILYYQGMVPP HIAAGINLNF WPAGPMFRRG GAFFIRRSFR 370 380 390 400 410 420 GNKLYTAVFR EYLDQLFAKG YSVEYFTEGG RSRTGRLLAP KTGMIAMTLN SVLRGVERPV 430 440 450 460 470 480 TLVPVYLGYD HVMEVATYHK ELSGKKKQKE SVWQVFGAIR KLGNFGQGYV NFGEPITLHS 490 500 510 520 530 540 YLNEQVPEWR EELAQDPEQK PSWLTPVVNT LANRVMTRIN DAAAVSSITL TSLVLLASEQ 550 560 570 580 590 600 NALQRSQLEK QLDLYLSLLK SVPYTGYTSV VEGDGAKLIA QGLELKKLKL DSDPLGDIIS 610 620 630 640 650 660 IDESLAIAMT YYRNNIIHLL VIPSLIASCL TRQDECSRDE IIATVKDFYP LLQAELFMGI 670 680 690 700 710 720 DNLETYVNQL LDQLLAEGLI DEDKHFSVKP DGLNQLLLLA GTISETMQRY AIIFNLLAAS 730 740 750 760 770 780 PKIERSELES DSHQLAQRLG ALHGITAPEF YDKKLYGTLS VKLKELGYLA DDSHSEDVQR 790 800 IRTRANSLLR SSVRQTIVDS VAAEHQA

References

[1]

"Complete sequence of Shewanella loihica PV-4." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Romine M.F., Serres G., Fredrickson J., Tiedje J., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1088 / PV-4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000606 Genomic DNA. Translation: ABO25396.1.
RefSeq	YP_001095655.1. NC_009092.1.
3D structure databases
ProteinModelPortal	A3QIU8.
ModBase	Search...
Protein-protein interaction databases
STRING	A3QIU8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4922863.
GenomeReviews	Gene locus Shew_3530 in contig CP000606_GR.
KEGG	slo:Shew_3530.
NMPDR	fig|323850.3.peg.1906.
PATRIC	23519007. VBISheLoi132094_3531.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2937.
HOGENOM	HBG296590.
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Enzyme and pathway databases
BioCyc	SLOI323850:SHEW_3530-MONOMER.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_SHELP

Accession

Primary (citable) accession number: A3QIU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 17, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program