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A3QH60

- LIPA_SHELP

UniProt

A3QH60 - LIPA_SHELP

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Protein
Lipoyl synthase
Gene
lipA, Shew_2942
Organism
Shewanella loihica (strain ATCC BAA-1088 / PV-4)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi94 – 941Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi98 – 981Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi101 – 1011Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSLOI323850:GHQJ-3042-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Shew_2942
OrganismiShewanella loihica (strain ATCC BAA-1088 / PV-4)
Taxonomic identifieri323850 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000001558: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 321321Lipoyl synthaseUniRule annotation
PRO_1000012276Add
BLAST

Proteomic databases

PRIDEiA3QH60.

Interactioni

Protein-protein interaction databases

STRINGi323850.Shew_2942.

Structurei

3D structure databases

ProteinModelPortaliA3QH60.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A3QH60-1 [UniParc]FASTAAdd to Basket

« Hide

MSRPERLQPG VKLRDADKVS RIPVKVVPSE RETMLRKPDW LRVKLPASNQ    50
RIVDIKQALR KNGLHSVCEE ASCPNLAECF NHGTATFMIL GAICTRRCPF 100
CDVAHGRPLK PDAEEPVKLA QTIRDMKLKY VVITSVDRDD LRDGGAQHFA 150
DCIREIRKLN PEIKIEILVP DFRGRIDAAL EILATEPPDV FNHNLETAPK 200
HYRKARPGAN YQWSLDLLKK FKEQHPHIPT KSGLMMGLGE TNEEIAEVLR 250
DLRAHNVEML TLGQYLQPSK FHLPVERYVP PAEFDELKDY AEEIGFTHAA 300
CGPMVRSSYH ADLQAQGKEV K 321
Length:321
Mass (Da):36,450
Last modified:April 17, 2007 - v1
Checksum:i332AAAF23A9AB4A7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000606 Genomic DNA. Translation: ABO24808.1.
RefSeqiYP_001095067.1. NC_009092.1.

Genome annotation databases

EnsemblBacteriaiABO24808; ABO24808; Shew_2942.
GeneIDi4924079.
KEGGislo:Shew_2942.
PATRICi23517795. VBISheLoi132094_2942.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000606 Genomic DNA. Translation: ABO24808.1 .
RefSeqi YP_001095067.1. NC_009092.1.

3D structure databases

ProteinModelPortali A3QH60.
ModBasei Search...

Protein-protein interaction databases

STRINGi 323850.Shew_2942.

Proteomic databases

PRIDEi A3QH60.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABO24808 ; ABO24808 ; Shew_2942 .
GeneIDi 4924079.
KEGGi slo:Shew_2942.
PATRICi 23517795. VBISheLoi132094_2942.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci SLOI323850:GHQJ-3042-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1088 / PV-4.

Entry informationi

Entry nameiLIPA_SHELP
AccessioniPrimary (citable) accession number: A3QH60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 17, 2007
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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