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A3QH47 (SYL_SHELP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Shew_2929
OrganismShewanella loihica (strain ATCC BAA-1088 / PV-4) [Complete proteome] [HAMAP]
Taxonomic identifier323850 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length863 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 863863Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009424

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif622 – 6265"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A3QH47 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: 8CD8A85373777D84

FASTA86397,736
        10         20         30         40         50         60 
MQEQYLPSEI EAKVQQHWQD TKTFEVTEDE SKEKFYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VVARYQRLQG KNVLQPIGWD SFGLPAENAA INNKTAPAPW TYENIDYMKN QLKMLGFGYD 

       130        140        150        160        170        180 
WSREIATCTP EYYRWEQWFF TKLYEKGLVY KKTASVNWCP NDETVLANEQ VQDGCCWRCD 

       190        200        210        220        230        240 
TQVVQKEIPQ WFIKITDYAE ELLNDIDQLD EWPEQVKTMQ RNWIGRSEGI EMTFQVEGSD 

       250        260        270        280        290        300 
QSFDIYTTRP DTVMGVTYVA IAAGHPLAQQ AAENNPVLAE FIEECKNADT TEAAMAAMEK 

       310        320        330        340        350        360 
KGVDTGLKAI HPLTGKLVPI WAANFVLMNY GTGAVMSVPG HDQRDYEFAT KYGLPIVGVI 

       370        380        390        400        410        420 
KPADGELDIS EEAYTEKGIL FNSGDSFPEL DGLDFQAAFD AIDAKLSSEG KGKRQVNYRL 

       430        440        450        460        470        480 
RDWGVSRQRY WGAPIPMVTL ADGTVMPTPE DQLPVILPED VVMDGIQSPI KADKEWAKTQ 

       490        500        510        520        530        540 
VNGQEAFRET DTFDTFMESS WYYARYCSPK ADQMLDPAKA NYWLPVDQYI GGIEHACMHL 

       550        560        570        580        590        600 
LYFRFFHKLL RDIGLVNSDE PAKRLLTQGM VLADAFYYTD DKGARVWVSP NDAKVTETDD 

       610        620        630        640        650        660 
KGRIVKAVDS QGHELVYTGM SKMSKSKNNG IDPQEMVDKY GADTVRLFMM FASPPELTLE 

       670        680        690        700        710        720 
WQESSVEGAH RFIKRLWKLA HDHVSQGPTV ALNVASLDSA QKELRRELHK TIAKVGDDIE 

       730        740        750        760        770        780 
RRQMFNTAIA SVMELMNRLQ KAPMESEQDR ALMQEALCAV VRLLYPIIPH TSFELWQALG 

       790        800        810        820        830        840 
HEETIENVLW PVVDESALVE DSKLIIVQVN GKLRAKVTVA ADADKDTVEA AGMAEEGVIK 

       850        860 
HTEGKTVRKV IYVPGKLLNI VAN 

« Hide

References

[1]"Complete sequence of Shewanella loihica PV-4."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Romine M.F., Serres G., Fredrickson J., Tiedje J., Richardson P.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1088 / PV-4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000606 Genomic DNA. Translation: ABO24795.1.
RefSeqYP_001095054.1. NC_009092.1.

3D structure databases

ProteinModelPortalA3QH47.
SMRA3QH47. Positions 228-416.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323850.Shew_2929.

Proteomic databases

PRIDEA3QH47.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABO24795; ABO24795; Shew_2929.
GeneID4922131.
KEGGslo:Shew_2929.
PATRIC23517769. VBISheLoi132094_2929.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycSLOI323850:GHQJ-3029-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_SHELP
AccessionPrimary (citable) accession number: A3QH47
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 17, 2007
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries