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A3QGU0

- PNP_SHELP

UniProt

A3QGU0 - PNP_SHELP

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Shewanella loihica (strain ATCC BAA-1088 / PV-4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (17 Apr 2007)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi485 – 4851MagnesiumUniRule annotation
    Metal bindingi491 – 4911MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciSLOI323850:GHQJ-2912-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:Shew_2822
    OrganismiShewanella loihica (strain ATCC BAA-1088 / PV-4)
    Taxonomic identifieri323850 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000001558: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 700700Polyribonucleotide nucleotidyltransferasePRO_0000329841Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

    Protein-protein interaction databases

    STRINGi323850.Shew_2822.

    Structurei

    3D structure databases

    ProteinModelPortaliA3QGU0.
    SMRiA3QGU0. Positions 616-691.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini552 – 61160KHUniRule annotationAdd
    BLAST
    Domaini621 – 68969S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218327.
    KOiK00962.
    OMAiPRWDWVA.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A3QGU0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNPIVKSFEY GQHTVTLETG VIARQADAAV LASMGDTTVL VTVVGKKAEE    50
    PGRDFFPLTV NYQEKTYAAG KIPGGFFKRE GRPSEGETLT ARLIDRPIRP 100
    LFPNGFKNEV QVIITVVSVD PEISPEVISM IGTSAALSIS GIPFNGPLGS 150
    ARVGYVDGEY ILNPTVSQLE NSQLELSVAG TENAVLMVES EADALPEEVM 200
    LGAVVYGHEQ QQVVIQAIKE LKAEVNKPMW DWTAPVQNEE LVAKVKDLAE 250
    AGLTEAYQIV VKQDRYAQVD VVKTAAKEAL LAENPDADAR EIDGLLGSLE 300
    KKVVRSRIIA GNPRIDGREP DMVRALSVMA GVLPRTHGSA LFTRGETQAL 350
    VTCTLGTERD AQKIDSIMGE QTSRFMLHYN FPPYSVGETG MVGSPKRREI 400
    GHGKLAWRGI HAVMPTAEEF PYSIRVVSEI TESNGSSSMA SVCGTSLALM 450
    DAGVPIKTSV AGIAMGLVKE GDDFVVLSDI LGDEDHLGDM DFKVAGTRDG 500
    VTALQMDIKI EGITKEIMQI ALQQAYGARV HILNVMDQAI SGHRAEISDH 550
    APRITTLKIN PEKIRDVIGK GGATIRALTE ETGTTIELED DGTVKIASAN 600
    GDATKEAIRR IQEITAEVEV GTVYNGKVVR IVDFGAFVTI LPGKDGLVHI 650
    SQIAEERVAN VSDYLEVGQE VKVKVMEVDR QGRVRLSMKE AQPKEEAASE 700
    Length:700
    Mass (Da):75,583
    Last modified:April 17, 2007 - v1
    Checksum:i9B87BA47F4992522
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000606 Genomic DNA. Translation: ABO24688.1.
    RefSeqiYP_001094947.1. NC_009092.1.

    Genome annotation databases

    EnsemblBacteriaiABO24688; ABO24688; Shew_2822.
    GeneIDi4922492.
    KEGGislo:Shew_2822.
    PATRICi23517533. VBISheLoi132094_2821.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000606 Genomic DNA. Translation: ABO24688.1 .
    RefSeqi YP_001094947.1. NC_009092.1.

    3D structure databases

    ProteinModelPortali A3QGU0.
    SMRi A3QGU0. Positions 616-691.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 323850.Shew_2822.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABO24688 ; ABO24688 ; Shew_2822 .
    GeneIDi 4922492.
    KEGGi slo:Shew_2822.
    PATRICi 23517533. VBISheLoi132094_2821.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218327.
    KOi K00962.
    OMAi PRWDWVA.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci SLOI323850:GHQJ-2912-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-1088 / PV-4.

    Entry informationi

    Entry nameiPNP_SHELP
    AccessioniPrimary (citable) accession number: A3QGU0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: April 17, 2007
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3