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A3QFP3

- FADJ_SHELP

UniProt

A3QFP3 - FADJ_SHELP

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Protein
Fatty acid oxidation complex subunit alpha
Gene
fadJ, Shew_2425
Organism
Shewanella loihica (strain ATCC BAA-1088 / PV-4)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities By similarity.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei116 – 1161Important for catalytic activity By similarity
Sitei138 – 1381Important for catalytic activity By similarity

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
  2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
  3. NAD binding Source: InterPro
  4. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciSLOI323850:GHQJ-2510-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alpha
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3)
3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
Gene namesi
Name:fadJ
Ordered Locus Names:Shew_2425
OrganismiShewanella loihica (strain ATCC BAA-1088 / PV-4)
Taxonomic identifieri323850 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000001558: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 706706Fatty acid oxidation complex subunit alphaUniRule annotation
PRO_0000323528Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI) By similarity.

Protein-protein interaction databases

STRINGi323850.Shew_2425.

Structurei

3D structure databases

ProteinModelPortaliA3QFP3.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 188188Enoyl-CoA hydratase By similarity
Add
BLAST
Regioni308 – 7063993-hydroxyacyl-CoA dehydrogenase By similarity
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261346.
KOiK01782.
OMAiPFRYMDT.
OrthoDBiEOG6M9F0M.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01617. FadJ.
InterProiIPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.

Sequencei

Sequence statusi: Complete.

A3QFP3-1 [UniParc]FASTAAdd to Basket

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MDKSFTLNRL DNGIAVLTMD VPGETMNTLR AEFGPEICEM LAEIKADAGI    50
RGVVIISGKK DSFVAGADIS MLDACATAED ARELSKQGHE VFFALESLSI 100
PVVAAINGAC LGGGLELALA CHQRVCTDSN KTMLGLPEVQ LGLLPGGGGT 150
QRLPRLVGIA KSLDMMLTGK QLRAKQALKM GLVDDVVPES ILLQTAIEMA 200
LAGARPAKKP KLSTVEKLLE GTPVGRNIIF EQALKQVNKK TQGNYPAPEK 250
IIDCVRQGVT KGIVKGLEVE AQHFGDLVVS NESEALRSIF FATTEMKKES 300
GAGDVSPKAV NKVMVLGGGL MGGGIASVTT TKAKIPVRVK DISEIGLSNA 350
LAYAYKLLAK GVKRRHMTPA VRDNLMALMT TTTEYKGIKD ADMVVEAVFE 400
DLNLKHQMVK DVERECGEHT IFASNTSSLP IKQIAEAAER PENVIGLHYF 450
SPVEKMPLVE VIAHDKTSPQ TIATTVAFAR KQGKTPIVVK DGAGFYVNRI 500
LALYMNEAAN LLLEGQSVDH LDKALVKFGF PVGPMTLLDE VGIDVGAKIS 550
PILEKELGER FKAPAAFDKL LADDRKGRKN GKGFYQYGAK SKKKLVDESV 600
YGVLGLTPGA DGEPIALAER CVVQMLNEAV RCLEEGIIAS PRDGDIGAIF 650
GIGFPPFLGG PFRYMDSLGA KHLVETLKRY QDQFGDRFAP CDRLVEMAES 700
GSKFYE 706
Length:706
Mass (Da):75,979
Last modified:April 17, 2007 - v1
Checksum:iACA8E59084E5B1E4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000606 Genomic DNA. Translation: ABO24291.1.
RefSeqiYP_001094550.1. NC_009092.1.

Genome annotation databases

EnsemblBacteriaiABO24291; ABO24291; Shew_2425.
GeneIDi4921646.
KEGGislo:Shew_2425.
PATRICi23516735. VBISheLoi132094_2425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000606 Genomic DNA. Translation: ABO24291.1 .
RefSeqi YP_001094550.1. NC_009092.1.

3D structure databases

ProteinModelPortali A3QFP3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 323850.Shew_2425.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABO24291 ; ABO24291 ; Shew_2425 .
GeneIDi 4921646.
KEGGi slo:Shew_2425.
PATRICi 23516735. VBISheLoi132094_2425.

Phylogenomic databases

eggNOGi COG1250.
HOGENOMi HOG000261346.
KOi K01782.
OMAi PFRYMDT.
OrthoDBi EOG6M9F0M.

Enzyme and pathway databases

UniPathwayi UPA00659 .
BioCyci SLOI323850:GHQJ-2510-MONOMER.

Family and domain databases

Gene3Di 1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPi MF_01617. FadJ.
InterProi IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsi TIGR02440. FadJ. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1088 / PV-4.

Entry informationi

Entry nameiFADJ_SHELP
AccessioniPrimary (citable) accession number: A3QFP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: April 17, 2007
Last modified: June 11, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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