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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Shewanella loihica (strain ATCC BAA-1088 / PV-4)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathway:ifatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei116 – 1161Important for catalytic activityUniRule annotation
Sitei138 – 1381Important for catalytic activityUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciSLOI323850:GHQJ-2510-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadJUniRule annotation
Ordered Locus Names:Shew_2425
OrganismiShewanella loihica (strain ATCC BAA-1088 / PV-4)
Taxonomic identifieri323850 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000001558 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 706706Fatty acid oxidation complex subunit alphaPRO_0000323528Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

Protein-protein interaction databases

STRINGi323850.Shew_2425.

Structurei

3D structure databases

ProteinModelPortaliA3QFP3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 188188Enoyl-CoA hydrataseUniRule annotationAdd
BLAST
Regioni308 – 7063993-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261346.
KOiK01782.
OMAiMMMLNEA.
OrthoDBiEOG6M9F0M.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01617. FadJ.
InterProiIPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.

Sequencei

Sequence statusi: Complete.

A3QFP3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKSFTLNRL DNGIAVLTMD VPGETMNTLR AEFGPEICEM LAEIKADAGI
60 70 80 90 100
RGVVIISGKK DSFVAGADIS MLDACATAED ARELSKQGHE VFFALESLSI
110 120 130 140 150
PVVAAINGAC LGGGLELALA CHQRVCTDSN KTMLGLPEVQ LGLLPGGGGT
160 170 180 190 200
QRLPRLVGIA KSLDMMLTGK QLRAKQALKM GLVDDVVPES ILLQTAIEMA
210 220 230 240 250
LAGARPAKKP KLSTVEKLLE GTPVGRNIIF EQALKQVNKK TQGNYPAPEK
260 270 280 290 300
IIDCVRQGVT KGIVKGLEVE AQHFGDLVVS NESEALRSIF FATTEMKKES
310 320 330 340 350
GAGDVSPKAV NKVMVLGGGL MGGGIASVTT TKAKIPVRVK DISEIGLSNA
360 370 380 390 400
LAYAYKLLAK GVKRRHMTPA VRDNLMALMT TTTEYKGIKD ADMVVEAVFE
410 420 430 440 450
DLNLKHQMVK DVERECGEHT IFASNTSSLP IKQIAEAAER PENVIGLHYF
460 470 480 490 500
SPVEKMPLVE VIAHDKTSPQ TIATTVAFAR KQGKTPIVVK DGAGFYVNRI
510 520 530 540 550
LALYMNEAAN LLLEGQSVDH LDKALVKFGF PVGPMTLLDE VGIDVGAKIS
560 570 580 590 600
PILEKELGER FKAPAAFDKL LADDRKGRKN GKGFYQYGAK SKKKLVDESV
610 620 630 640 650
YGVLGLTPGA DGEPIALAER CVVQMLNEAV RCLEEGIIAS PRDGDIGAIF
660 670 680 690 700
GIGFPPFLGG PFRYMDSLGA KHLVETLKRY QDQFGDRFAP CDRLVEMAES

GSKFYE
Length:706
Mass (Da):75,979
Last modified:April 17, 2007 - v1
Checksum:iACA8E59084E5B1E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000606 Genomic DNA. Translation: ABO24291.1.
RefSeqiWP_011866222.1. NC_009092.1.

Genome annotation databases

EnsemblBacteriaiABO24291; ABO24291; Shew_2425.
KEGGislo:Shew_2425.
PATRICi23516735. VBISheLoi132094_2425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000606 Genomic DNA. Translation: ABO24291.1.
RefSeqiWP_011866222.1. NC_009092.1.

3D structure databases

ProteinModelPortaliA3QFP3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323850.Shew_2425.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO24291; ABO24291; Shew_2425.
KEGGislo:Shew_2425.
PATRICi23516735. VBISheLoi132094_2425.

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261346.
KOiK01782.
OMAiMMMLNEA.
OrthoDBiEOG6M9F0M.

Enzyme and pathway databases

UniPathwayiUPA00659.
BioCyciSLOI323850:GHQJ-2510-MONOMER.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01617. FadJ.
InterProiIPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1088 / PV-4.

Entry informationi

Entry nameiFADJ_SHELP
AccessioniPrimary (citable) accession number: A3QFP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: April 17, 2007
Last modified: July 22, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.