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A3QFP3

- FADJ_SHELP

UniProt

A3QFP3 - FADJ_SHELP

Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Shewanella loihica (strain ATCC BAA-1088 / PV-4)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (17 Apr 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

    Catalytic activityi

    (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
    (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
    (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei116 – 1161Important for catalytic activityUniRule annotation
    Sitei138 – 1381Important for catalytic activityUniRule annotation

    GO - Molecular functioni

    1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
    2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
    3. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP
    4. NAD binding Source: InterPro

    GO - Biological processi

    1. fatty acid beta-oxidation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase, Lyase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciSLOI323850:GHQJ-2510-MONOMER.
    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fatty acid oxidation complex subunit alphaUniRule annotation
    Including the following 2 domains:
    Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
    3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
    Gene namesi
    Name:fadJUniRule annotation
    Ordered Locus Names:Shew_2425
    OrganismiShewanella loihica (strain ATCC BAA-1088 / PV-4)
    Taxonomic identifieri323850 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000001558: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 706706Fatty acid oxidation complex subunit alphaPRO_0000323528Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

    Protein-protein interaction databases

    STRINGi323850.Shew_2425.

    Structurei

    3D structure databases

    ProteinModelPortaliA3QFP3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 188188Enoyl-CoA hydrataseUniRule annotationAdd
    BLAST
    Regioni308 – 7063993-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
    In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1250.
    HOGENOMiHOG000261346.
    KOiK01782.
    OMAiPFRYMDT.
    OrthoDBiEOG6M9F0M.

    Family and domain databases

    Gene3Di1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPiMF_01617. FadJ.
    InterProiIPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsiTIGR02440. FadJ. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A3QFP3-1 [UniParc]FASTAAdd to Basket

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    MDKSFTLNRL DNGIAVLTMD VPGETMNTLR AEFGPEICEM LAEIKADAGI    50
    RGVVIISGKK DSFVAGADIS MLDACATAED ARELSKQGHE VFFALESLSI 100
    PVVAAINGAC LGGGLELALA CHQRVCTDSN KTMLGLPEVQ LGLLPGGGGT 150
    QRLPRLVGIA KSLDMMLTGK QLRAKQALKM GLVDDVVPES ILLQTAIEMA 200
    LAGARPAKKP KLSTVEKLLE GTPVGRNIIF EQALKQVNKK TQGNYPAPEK 250
    IIDCVRQGVT KGIVKGLEVE AQHFGDLVVS NESEALRSIF FATTEMKKES 300
    GAGDVSPKAV NKVMVLGGGL MGGGIASVTT TKAKIPVRVK DISEIGLSNA 350
    LAYAYKLLAK GVKRRHMTPA VRDNLMALMT TTTEYKGIKD ADMVVEAVFE 400
    DLNLKHQMVK DVERECGEHT IFASNTSSLP IKQIAEAAER PENVIGLHYF 450
    SPVEKMPLVE VIAHDKTSPQ TIATTVAFAR KQGKTPIVVK DGAGFYVNRI 500
    LALYMNEAAN LLLEGQSVDH LDKALVKFGF PVGPMTLLDE VGIDVGAKIS 550
    PILEKELGER FKAPAAFDKL LADDRKGRKN GKGFYQYGAK SKKKLVDESV 600
    YGVLGLTPGA DGEPIALAER CVVQMLNEAV RCLEEGIIAS PRDGDIGAIF 650
    GIGFPPFLGG PFRYMDSLGA KHLVETLKRY QDQFGDRFAP CDRLVEMAES 700
    GSKFYE 706
    Length:706
    Mass (Da):75,979
    Last modified:April 17, 2007 - v1
    Checksum:iACA8E59084E5B1E4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000606 Genomic DNA. Translation: ABO24291.1.
    RefSeqiYP_001094550.1. NC_009092.1.

    Genome annotation databases

    EnsemblBacteriaiABO24291; ABO24291; Shew_2425.
    GeneIDi4921646.
    KEGGislo:Shew_2425.
    PATRICi23516735. VBISheLoi132094_2425.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000606 Genomic DNA. Translation: ABO24291.1 .
    RefSeqi YP_001094550.1. NC_009092.1.

    3D structure databases

    ProteinModelPortali A3QFP3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 323850.Shew_2425.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABO24291 ; ABO24291 ; Shew_2425 .
    GeneIDi 4921646.
    KEGGi slo:Shew_2425.
    PATRICi 23516735. VBISheLoi132094_2425.

    Phylogenomic databases

    eggNOGi COG1250.
    HOGENOMi HOG000261346.
    KOi K01782.
    OMAi PFRYMDT.
    OrthoDBi EOG6M9F0M.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .
    BioCyci SLOI323850:GHQJ-2510-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPi MF_01617. FadJ.
    InterProi IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsi TIGR02440. FadJ. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-1088 / PV-4.

    Entry informationi

    Entry nameiFADJ_SHELP
    AccessioniPrimary (citable) accession number: A3QFP3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: April 17, 2007
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3