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A3QEV1 (DDL_SHELP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Shew_2133
OrganismShewanella loihica (strain ATCC BAA-1088 / PV-4) [Complete proteome] [HAMAP]
Taxonomic identifier323850 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000074789

Regions

Domain124 – 329206ATP-grasp
Nucleotide binding154 – 20956ATP By similarity

Sites

Metal binding2831Magnesium or manganese 1 By similarity
Metal binding2961Magnesium or manganese 1 By similarity
Metal binding2961Magnesium or manganese 2 By similarity
Metal binding2981Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A3QEV1 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: A06FAF455CE300A2

FASTA33437,044
        10         20         30         40         50         60 
MNPINLLLLC GGGGDEHAIS LLSANYFETS LAKLPHLNVL RVELDAKGQY QSKSGERCEL 

        70         80         90        100        110        120 
TNRREIRFED ESLAPWPVDY VIPCIHGYPG ETGDIQSYFE LINLPYFGCD AEGSRNCFNK 

       130        140        150        160        170        180 
ITAKMWFSAL GIPNTPYLFL NQLDEQAIAQ TTDALAKWGS VFVKAASQGS SVGCYRVDDA 

       190        200        210        220        230        240 
AQIPAILADA FHYSPYVVVE KTIQARELEV AVYQYRGETV ATLPGEVICA GGTFYTYDEK 

       250        260        270        280        290        300 
YAADSKATTK VVAEVSDEIA QQIRAYAVKV FDGMKLRHLS RIDFFLTPEG EILLNEINTF 

       310        320        330 
PGLTPISMFP KMLANHGDDF SQYLNEAILT GLNR 

« Hide

References

[1]"Complete sequence of Shewanella loihica PV-4."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Romine M.F., Serres G., Fredrickson J., Tiedje J., Richardson P.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1088 / PV-4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000606 Genomic DNA. Translation: ABO23999.1.
RefSeqYP_001094258.1. NC_009092.1.

3D structure databases

ProteinModelPortalA3QEV1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323850.Shew_2133.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABO23999; ABO23999; Shew_2133.
GeneID4923499.
KEGGslo:Shew_2133.
PATRIC23516139. VBISheLoi132094_2132.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMARSAYGIY.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycSLOI323850:GHQJ-2213-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_SHELP
AccessionPrimary (citable) accession number: A3QEV1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 17, 2007
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways