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Protein

Aspartate--tRNA ligase

Gene

aspS

Organism
Shewanella loihica (strain ATCC BAA-1088 / PV-4)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp).UniRule annotation

Catalytic activityi

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei173AspartateUniRule annotation1
Binding sitei219AspartateUniRule annotation1
Binding sitei228ATPUniRule annotation1
Binding sitei449AspartateUniRule annotation1
Binding sitei483ATPUniRule annotation1
Binding sitei490AspartateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi219 – 221ATPUniRule annotation3
Nucleotide bindingi535 – 538ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSLOI323850:G1G8D-2202-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA ligaseUniRule annotation (EC:6.1.1.12UniRule annotation)
Alternative name(s):
Aspartyl-tRNA synthetaseUniRule annotation
Short name:
AspRSUniRule annotation
Gene namesi
Name:aspSUniRule annotation
Ordered Locus Names:Shew_2079
OrganismiShewanella loihica (strain ATCC BAA-1088 / PV-4)
Taxonomic identifieri323850 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000001558 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000067571 – 592Aspartate--tRNA ligaseAdd BLAST592

Proteomic databases

PRIDEiA3QEP7.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi323850.Shew_2079.

Structurei

3D structure databases

ProteinModelPortaliA3QEP7.
SMRiA3QEP7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni197 – 200AspartateUniRule annotation4

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9M. Bacteria.
COG0173. LUCA.
HOGENOMiHOG000275160.
KOiK01876.
OMAiYQLDVEM.
OrthoDBiPOG091H021G.

Family and domain databases

Gene3Di3.30.1360.30. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR004364. aa-tRNA-synt_II.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_1.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like_sf.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
PANTHERiPTHR22594:SF5. PTHR22594:SF5. 1 hit.
PfamiView protein in Pfam
PF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiView protein in PROSITE
PS50862. AA_TRNA_LIGASE_II. 1 hit.

Sequencei

Sequence statusi: Complete.

A3QEP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSHYCGDVN RSHVGEEVTL VGWVNRSRDL GGVVFLDLRD REGVIQVVYD
60 70 80 90 100
PDLPEVFDVA STLRSEFCVQ VKGLVRARPD SQINDQMRTG EIEVLGKALT
110 120 130 140 150
ILNSAPALPI NMDKNQHNTE EQRLKYRYLD LRRPEMAERI IFRSKVTSAV
160 170 180 190 200
RRFLDGNGFL DIETPILTKA TPEGARDYLV PSRTYKGQFF ALPQSPQLFK
210 220 230 240 250
QLLMMSGFDR YYQIVKCFRD EDLRADRQPE FTQIDIETSF MTSAQVMDKT
260 270 280 290 300
EEMVRGLFKE LLNVDLGEFP KMTFAEAMRR YGSDKPDLRN PLELVDVADL
310 320 330 340 350
VKDVDFKVFQ EPANDSEGRV AVLCVPGGAS LSRKQLDEYG KYVNIYGAKG
360 370 380 390 400
LAWMKVNELE NGLEGIQSPV LKFLSEEVVK GILERTGAAN GDLILFGADK
410 420 430 440 450
ANIVAEAMGA LRLKVGEDFD LLQGDWKPLW VVDFPMFERT SDGGLHAMHH
460 470 480 490 500
PFTAPSGITP AELEADPTAA ISDAYDMVLN GCELGGGSVR IYDAEMQSAV
510 520 530 540 550
FRILGINDEE AQEKFGFLLE ALKYGTPPHA GLAFGLDRMV MLMTGASSIR
560 570 580 590
DVMAFPKTTT AACPLTNAPG FANPVQLEEL GVSVVEAKKE QE
Length:592
Mass (Da):65,939
Last modified:April 17, 2007 - v1
Checksum:iD522065BAA27EE7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000606 Genomic DNA. Translation: ABO23945.1.
RefSeqiWP_011865877.1. NC_009092.1.

Genome annotation databases

EnsemblBacteriaiABO23945; ABO23945; Shew_2079.
KEGGislo:Shew_2079.

Similar proteinsi

Entry informationi

Entry nameiSYD_SHELP
AccessioniPrimary (citable) accession number: A3QEP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 17, 2007
Last modified: February 28, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome