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A3QDY0 (RLMKL_SHELP) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA large subunit methyltransferase K/L

Including the following 2 domains:

  1. 23S rRNA m2G2445 methyltransferase
    EC=2.1.1.173
    Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase RlmL
  2. 23S rRNA m7G2069 methyltransferase
    EC=2.1.1.264
    Alternative name(s):
    rRNA (guanine-N(7)-)-methyltransferase RlmK
Gene names
Name:rlmL
Ordered Locus Names:Shew_1812
OrganismShewanella loihica (strain ATCC BAA-1088 / PV-4) [Complete proteome] [HAMAP]
Taxonomic identifier323850 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length711 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA By similarity. HAMAP-Rule MF_01858

Catalytic activity

S-adenosyl-L-methionine + guanine(2445) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(2445) in 23S rRNA. HAMAP-Rule MF_01858

S-adenosyl-L-methionine + guanine(2069) in 23S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(2069) in 23S rRNA. HAMAP-Rule MF_01858

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01858.

Sequence similarities

Belongs to the methyltransferase superfamily. RlmKL family.

Contains 1 THUMP domain.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandRNA-binding
S-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function23S rRNA (guanine(2445)-N(2))-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

RNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

rRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 711711Ribosomal RNA large subunit methyltransferase K/L HAMAP-Rule MF_01858
PRO_0000366826

Regions

Domain43 – 154112THUMP

Sequences

Sequence LengthMass (Da)Tools
A3QDY0 [UniParc].

Last modified April 17, 2007. Version 1.
Checksum: 8BC6CAFE330CA57C

FASTA71179,892
        10         20         30         40         50         60 
MLNFFAAAPR GYEYALSLEL AEFGAAEIKE SVAGVYFSAP LNLAYRITLW SRLASRIILV 

        70         80         90        100        110        120 
IYKGPCENPE QLYNAAYCID WQTHFSNKSS FSIDFHGVGG FIKNSQFGAL KIKDAVVDRF 

       130        140        150        160        170        180 
RDDGCPRPDV ARVDADFKID AHYRRGQITL GINFSGPALH QRGYRSTTGE APLKENLAAN 

       190        200        210        220        230        240 
MLVRSGWQQS PKDLLDPFCG SGTILIEAAM MACDIAPALQ RRRFGFEHWL RHQEADWQEL 

       250        260        270        280        290        300 
LAEAKARASI GTKRCEIKFY GSDIDSRLVA LAKRNAQNAG VAELIELSVS NALNVTPPVE 

       310        320        330        340        350        360 
QGYLITNPPY GERLGNVTSL LQLYYQLGDK LKAEFGGWQV AVLNSDIELL SALKLKADKQ 

       370        380        390        400        410        420 
MKMYNGALEC AFNLYTLHAN STRRLDPSQV LSQGGEVSEV ATAFSNRIKK NHKQLSKWAQ 

       430        440        450        460        470        480 
REGIDSYRLY DADIPEYNVA VDIYLDHVVI QEYSAPKSIP EAVTKRRLTD VLLVLPQAIG 

       490        500        510        520        530        540 
VDPDKIILKT RERQKGTNQY QKLDATKLEL VTTEYGAKFK LNLKDYLDTG LFLDHRLTRK 

       550        560        570        580        590        600 
LVGEKSKGRD VLNLFAYTGS ASVHAALGGA KSVTTVDMSN TYLNWAQDNF ELNGLKGKQY 

       610        620        630        640        650        660 
QFIQGDCLQW IDDCDQQYDL IFIDPPTFSN SKRMEDSFDV QRDHVKLLSA LVKLLRPGGE 

       670        680        690        700        710 
ILFSNNKRKF KMDSEALSAL GLSITNLDKQ TLPLDYKRNP HIHNTWLIQH A 

« Hide

References

[1]"Complete sequence of Shewanella loihica PV-4."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Romine M.F., Serres G., Fredrickson J., Tiedje J., Richardson P.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1088 / PV-4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000606 Genomic DNA. Translation: ABO23678.1.
RefSeqYP_001093937.1. NC_009092.1.

3D structure databases

ProteinModelPortalA3QDY0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323850.Shew_1812.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABO23678; ABO23678; Shew_1812.
GeneID4921704.
KEGGslo:Shew_1812.
PATRIC23515453. VBISheLoi132094_1809.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0116.
HOGENOMHOG000218371.
KOK12297.
OMANKRGFKM.
OrthoDBEOG6HJ238.

Enzyme and pathway databases

BioCycSLOI323850:GHQJ-1872-MONOMER.

Family and domain databases

Gene3D3.40.50.150. 3 hits.
HAMAPMF_01858. 23SrRNA_methyltr_KL.
InterProIPR017244. 23SrRNA_methyltr_KL.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR000241. RNA_methylase_dom.
IPR019614. SAM-dep_methyl-trfase.
IPR029063. SAM-dependent_MTases-like.
IPR004114. THUMP.
[Graphical view]
PfamPF10672. Methyltrans_SAM. 1 hit.
PF02926. THUMP. 1 hit.
PF01170. UPF0020. 1 hit.
[Graphical view]
PIRSFPIRSF037618. RNA_Mtase_bacteria_prd. 1 hit.
SMARTSM00981. THUMP. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 3 hits.
PROSITEPS51165. THUMP. 1 hit.
PS01261. UPF0020. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRLMKL_SHELP
AccessionPrimary (citable) accession number: A3QDY0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 17, 2007
Last modified: June 11, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families