A3QCW8 (SYE_SHELP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Shew_1449

Organism

Shewanella loihica (strain ATCC BAA-1088 / PV-4) [Complete proteome] [HAMAP]

Taxonomic identifier

323850 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella › Shewanella loihica

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00022
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00022
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Glutamate--tRNA ligase HAMAP-Rule MF_00022		PRO_1000001959
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP-Rule MF_00022
	Motif	236 – 240	5	"KMSKS" region HAMAP-Rule MF_00022
Sites
	Metal binding	98	1	Zinc By similarity
	Metal binding	100	1	Zinc By similarity
	Metal binding	125	1	Zinc By similarity
	Metal binding	127	1	Zinc By similarity
	Binding site	239	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3QCW8 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 8DBD88175C499923	FASTA	469	52,921
	10 20 30 40 50 60 MTIKTRFAPS PTGFLHVGGA RTALYSWLYA RANQGEFVLR IEDTDLERST PEACAAILEG 70 80 90 100 110 120 MEWLGLNWDE GPYYQTKRFD RYNEIIAQML EQGTAYKCYC SRERIEAMRE EQAAKGEQQK 130 140 150 160 170 180 YDGCCRDKAP RDTDEPFVVR FKNPTEGSVV FDDHVRGRIE ISNELLDDLI IARTDGTPTY 190 200 210 220 230 240 NFCVVVDDWD MGITCVVRGE DHINNTPRQI NILKALNAPV PEYAHVAMIL GDDGAKLSKR 250 260 270 280 290 300 HGAVGVMQYR DDGYLPEALL NYLVRLGWSH GDQEVFSIEE MKQYFKLDDI NKAASAFNTE 310 320 330 340 350 360 KLIWLNQHYM KELAPEYVAK HLEWHMQDQN IDTANGPALA EVVSALAERA KTLKELAASS 370 380 390 400 410 420 RYFYEDFAEF DETAAKKHLR GVALEPLQLL QKKFAELSEW NVEAIHQAIE ATAAELELGM 430 440 450 460 GKVGMPLRVA VTGAGMSPAV DLTAFLVGKA RCEQRISKAI EFVANRINS

References

[1]

"Complete sequence of Shewanella loihica PV-4." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Romine M.F., Serres G., Fredrickson J., Tiedje J., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1088 / PV-4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000606 Genomic DNA. Translation: ABO23316.1.
RefSeq	YP_001093575.1. NC_009092.1.
3D structure databases
ProteinModelPortal	A3QCW8.
ModBase	Search...
Protein-protein interaction databases
STRING	323850.Shew_1449.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABO23316; ABO23316; Shew_1449.
GeneID	4922583.
KEGG	slo:Shew_1449.
PATRIC	23514699. VBISheLoi132094_1453.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000252722.
KO	K01885.
OMA	EEWISST.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	SLOI323850:GHQJ-1488-MONOMER.
Family and domain databases
Gene3D	1.10.10.350. 1 hit. 1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_B. Glu_tRNA_synth_B.
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-ligase_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_SHELP

Accession

Primary (citable) accession number: A3QCW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	May 29, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program