UniProtKB - A3QCW8 (SYE_SHELP)
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Protein
Glutamate--tRNA ligase
Gene
gltX
Organism
Shewanella loihica (strain ATCC BAA-1088 / PV-4)
Status
Functioni
Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation
Catalytic activityi
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation
Cofactori
Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 98 | ZincUniRule annotation | 1 | |
Metal bindingi | 100 | ZincUniRule annotation | 1 | |
Metal bindingi | 125 | ZincUniRule annotation | 1 | |
Metal bindingi | 127 | ZincUniRule annotation | 1 | |
Binding sitei | 239 | ATPUniRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- glutamate-tRNA ligase activity Source: UniProtKB-EC
- tRNA binding Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- glutamyl-tRNA aminoacylation Source: InterPro
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | SLOI323850:G1G8D-1527-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)Alternative name(s): Glutamyl-tRNA synthetaseUniRule annotation Short name: GluRSUniRule annotation |
Gene namesi | Name:gltXUniRule annotation Ordered Locus Names:Shew_1449 |
Organismi | Shewanella loihica (strain ATCC BAA-1088 / PV-4) |
Taxonomic identifieri | 323850 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000001959 | 1 – 469 | Glutamate--tRNA ligaseAdd BLAST | 469 |
Interactioni
Subunit structurei
Monomer.UniRule annotation
Protein-protein interaction databases
STRINGi | 323850.Shew_1449 |
Structurei
3D structure databases
ProteinModelPortali | A3QCW8 |
SMRi | A3QCW8 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 9 – 19 | "HIGH" regionUniRule annotationAdd BLAST | 11 | |
Motifi | 236 – 240 | "KMSKS" regionUniRule annotation | 5 |
Sequence similaritiesi
Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4105C20 Bacteria COG0008 LUCA |
HOGENOMi | HOG000252722 |
KOi | K01885 |
OMAi | HYINTLP |
OrthoDBi | POG091H021W |
Family and domain databases
CDDi | cd00808 GluRS_core, 1 hit |
Gene3Di | 1.10.10.3501 hit 3.40.50.6201 hit |
HAMAPi | MF_00022 Glu_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR008925 aa-tRNA-synth_I_codon-bd IPR020751 aa-tRNA-synth_I_codon-bd_sub2 IPR001412 aa-tRNA-synth_I_CS IPR004527 Glu-tRNA-ligase_bac/mito IPR000924 Glu/Gln-tRNA-synth IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom IPR033910 GluRS_core IPR014729 Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF00749 tRNA-synt_1c, 1 hit |
PRINTSi | PR00987 TRNASYNTHGLU |
SUPFAMi | SSF48163 SSF48163, 1 hit |
TIGRFAMsi | TIGR00464 gltX_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00178 AA_TRNA_LIGASE_I, 1 hit |
i Sequence
Sequence statusi: Complete.
A3QCW8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTIKTRFAPS PTGFLHVGGA RTALYSWLYA RANQGEFVLR IEDTDLERST
60 70 80 90 100
PEACAAILEG MEWLGLNWDE GPYYQTKRFD RYNEIIAQML EQGTAYKCYC
110 120 130 140 150
SRERIEAMRE EQAAKGEQQK YDGCCRDKAP RDTDEPFVVR FKNPTEGSVV
160 170 180 190 200
FDDHVRGRIE ISNELLDDLI IARTDGTPTY NFCVVVDDWD MGITCVVRGE
210 220 230 240 250
DHINNTPRQI NILKALNAPV PEYAHVAMIL GDDGAKLSKR HGAVGVMQYR
260 270 280 290 300
DDGYLPEALL NYLVRLGWSH GDQEVFSIEE MKQYFKLDDI NKAASAFNTE
310 320 330 340 350
KLIWLNQHYM KELAPEYVAK HLEWHMQDQN IDTANGPALA EVVSALAERA
360 370 380 390 400
KTLKELAASS RYFYEDFAEF DETAAKKHLR GVALEPLQLL QKKFAELSEW
410 420 430 440 450
NVEAIHQAIE ATAAELELGM GKVGMPLRVA VTGAGMSPAV DLTAFLVGKA
460
RCEQRISKAI EFVANRINS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000606 Genomic DNA Translation: ABO23316.1 |
RefSeqi | WP_011865248.1, NC_009092.1 |
Genome annotation databases
EnsemblBacteriai | ABO23316; ABO23316; Shew_1449 |
KEGGi | slo:Shew_1449 |
Similar proteinsi
Entry informationi
Entry namei | SYE_SHELP | |
Accessioni | A3QCW8Primary (citable) accession number: A3QCW8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | April 3, 2007 | |
Last modified: | March 28, 2018 | |
This is version 78 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |