Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Gene

ispD

Organism
Shewanella loihica (strain ATCC BAA-1088 / PV-4)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).UniRule annotation

Catalytic activityi

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 2 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei20Transition state stabilizerUniRule annotation1
Sitei27Transition state stabilizerUniRule annotation1
Sitei159Positions MEP for the nucleophilic attackUniRule annotation1
Sitei215Positions MEP for the nucleophilic attackUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Isoprene biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00056; UER00093.

Names & Taxonomyi

Protein namesi
Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferaseUniRule annotation (EC:2.7.7.60UniRule annotation)
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthaseUniRule annotation
MEP cytidylyltransferaseUniRule annotation
Short name:
MCTUniRule annotation
Gene namesi
Name:ispDUniRule annotation
Ordered Locus Names:Shew_1207
OrganismiShewanella loihica (strain ATCC BAA-1088 / PV-4)
Taxonomic identifieri323850 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000001558 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000713221 – 2422-C-methyl-D-erythritol 4-phosphate cytidylyltransferaseAdd BLAST242

Interactioni

Protein-protein interaction databases

STRINGi323850.Shew_1207.

Structurei

3D structure databases

ProteinModelPortaliA3QC79.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE5. Bacteria.
COG1211. LUCA.
HOGENOMiHOG000218564.
KOiK00991.
OMAiQAYTPQM.
OrthoDBiPOG091H066R.

Family and domain databases

CDDicd02516. CDP-ME_synthetase. 1 hit.
Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD. 1 hit.
InterProiIPR001228. IspD.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR00453. ispD. 1 hit.

Sequencei

Sequence statusi: Complete.

A3QC79-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISRIQELVA IVPAAGIGAR MGAEIPKQYL MLNHQPILAH TLDRLLEHPR
60 70 80 90 100
IDKVIIALSP EDSHFAKLSQ ASHPKLMTVI GGKERADSVL SALKVASQSA
110 120 130 140 150
WALVHDAARP CLSAADIDKL IAACETEEGH QQGAILAMPV RDTMKRSGKG
160 170 180 190 200
GAIQETVCRE NLWHALTPQL FPVISLKQNL ADALAAQVAI TDEASAMEWA
210 220 230 240
GGQPLLVSGR FDNIKVTHPE DLQLAALYLQ AAASDPNSST GE
Length:242
Mass (Da):25,909
Last modified:April 17, 2007 - v1
Checksum:i935670E302877AC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000606 Genomic DNA. Translation: ABO23077.1.
RefSeqiWP_011865009.1. NC_009092.1.

Genome annotation databases

EnsemblBacteriaiABO23077; ABO23077; Shew_1207.
KEGGislo:Shew_1207.
PATRICi23514193. VBISheLoi132094_1212.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000606 Genomic DNA. Translation: ABO23077.1.
RefSeqiWP_011865009.1. NC_009092.1.

3D structure databases

ProteinModelPortaliA3QC79.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323850.Shew_1207.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO23077; ABO23077; Shew_1207.
KEGGislo:Shew_1207.
PATRICi23514193. VBISheLoi132094_1212.

Phylogenomic databases

eggNOGiENOG4105CE5. Bacteria.
COG1211. LUCA.
HOGENOMiHOG000218564.
KOiK00991.
OMAiQAYTPQM.
OrthoDBiPOG091H066R.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00093.

Family and domain databases

CDDicd02516. CDP-ME_synthetase. 1 hit.
Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD. 1 hit.
InterProiIPR001228. IspD.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR00453. ispD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiISPD_SHELP
AccessioniPrimary (citable) accession number: A3QC79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: April 17, 2007
Last modified: November 2, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.