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A3QBX2

- SYI_SHELP

UniProt

A3QBX2 - SYI_SHELP

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Shewanella loihica (strain ATCC BAA-1088 / PV-4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (17 Apr 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
    Binding sitei608 – 6081ATPUniRule annotation
    Metal bindingi903 – 9031ZincUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi923 – 9231ZincUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSLOI323850:GHQJ-1122-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Shew_1099
    OrganismiShewanella loihica (strain ATCC BAA-1088 / PV-4)
    Taxonomic identifieri323850 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000001558: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 940940Isoleucine--tRNA ligasePRO_1000022118Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi323850.Shew_1099.

    Structurei

    3D structure databases

    ProteinModelPortaliA3QBX2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi605 – 6095"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A3QBX2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKSTLNL PETEFPMRGN LAQREPEMLK SWTEKKLYQK IRESRTGAKP    50
    FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKTLSGFDAP YIPGWDCHGL 100
    PIELKVEQKV GKPGQKVSAA EFRQKCREYA AAQVDGQRED FIRLGVLGDW 150
    QNPYLTMNFD TEANIVRSLA KVIDNGHLHK GVKPVHWCTD CGSALAEAEV 200
    EYEDKTSPAI DVAFVAVDQA AVLAKFGLEQ SDAEVATVIW TTTPWTLPAN 250
    RGLSMAGDID YDLVEFTKDG SKRAIIVAQE LVESCIERYG AELHSVLGTV 300
    KGQALELLRF KHPFYDFDVP VIIGDHVTVD SGTGVVHTAP GHGQDDFVIG 350
    QKYGLEVANP VGDNGVYKPD TEFFAGQHVF KANDNVVALL EEKGALLNHV 400
    AYRHSYPHCW RHKTPIIFRA TPQWFISMDQ KGLRTQSLSE IEKTQWIPDW 450
    GQSRIEKMVE NRPDWCISRQ RTWGVPITLF VHRDTEELHP DSVALMERVA 500
    HRIEQEGIQA WWDLDAAELL GEEADQYRKV TDTLDVWYDS GSTFSSVVAA 550
    RPEYNGHPVD LYLEGSDQHR GWFMSSLMIS TAMNGKAPYK QVLTHGFTVD 600
    GQGRKMSKSI GNVIAPLQVT NKLGADILRL WVAATDYSGE MTVSDEILKR 650
    SADAYRRIRN TARFLLANLN GFDPVNDTVA IEDMVALDRW VVRRAAALQA 700
    EILEAYEQYN FHLVTQKLMQ FCSVELGSFY LDIIKDRQYT AKRDSLARRS 750
    CQSALFLIAE AMVRWIAPIL SFTADEVWQS LPGEREEFVF TQTWFEGLVS 800
    VTLDSDLSDD YWANLITVRD EVNKAIEQAR REQRVGGSLE AEVTIYADEA 850
    LTESLALLGD ELRFVLLTSN TQIAPLANAG DDAVETELAG LKLLVDKTEA 900
    AKCDRCWHHR EDVGQVEAHP LLCTRCVTNI EGDGEVRQFA 940
    Length:940
    Mass (Da):105,584
    Last modified:April 17, 2007 - v1
    Checksum:i5B315B649E3AA0E1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000606 Genomic DNA. Translation: ABO22970.1.
    RefSeqiYP_001093229.1. NC_009092.1.

    Genome annotation databases

    EnsemblBacteriaiABO22970; ABO22970; Shew_1099.
    GeneIDi4923352.
    KEGGislo:Shew_1099.
    PATRICi23513975. VBISheLoi132094_1105.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000606 Genomic DNA. Translation: ABO22970.1 .
    RefSeqi YP_001093229.1. NC_009092.1.

    3D structure databases

    ProteinModelPortali A3QBX2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 323850.Shew_1099.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABO22970 ; ABO22970 ; Shew_1099 .
    GeneIDi 4923352.
    KEGGi slo:Shew_1099.
    PATRICi 23513975. VBISheLoi132094_1105.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci SLOI323850:GHQJ-1122-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-1088 / PV-4.

    Entry informationi

    Entry nameiSYI_SHELP
    AccessioniPrimary (citable) accession number: A3QBX2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: April 17, 2007
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3