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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Shewanella loihica (strain ATCC BAA-1088 / PV-4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
Binding sitei608 – 6081ATPUniRule annotation
Metal bindingi903 – 9031ZincUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi923 – 9231ZincUniRule annotation
Metal bindingi926 – 9261ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSLOI323850:GHQJ-1122-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Shew_1099
OrganismiShewanella loihica (strain ATCC BAA-1088 / PV-4)
Taxonomic identifieri323850 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000001558: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 940940Isoleucine--tRNA ligasePRO_1000022118Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi323850.Shew_1099.

Structurei

3D structure databases

ProteinModelPortaliA3QBX2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi605 – 6095"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3QBX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYKSTLNL PETEFPMRGN LAQREPEMLK SWTEKKLYQK IRESRTGAKP
60 70 80 90 100
FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKTLSGFDAP YIPGWDCHGL
110 120 130 140 150
PIELKVEQKV GKPGQKVSAA EFRQKCREYA AAQVDGQRED FIRLGVLGDW
160 170 180 190 200
QNPYLTMNFD TEANIVRSLA KVIDNGHLHK GVKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYEDKTSPAI DVAFVAVDQA AVLAKFGLEQ SDAEVATVIW TTTPWTLPAN
260 270 280 290 300
RGLSMAGDID YDLVEFTKDG SKRAIIVAQE LVESCIERYG AELHSVLGTV
310 320 330 340 350
KGQALELLRF KHPFYDFDVP VIIGDHVTVD SGTGVVHTAP GHGQDDFVIG
360 370 380 390 400
QKYGLEVANP VGDNGVYKPD TEFFAGQHVF KANDNVVALL EEKGALLNHV
410 420 430 440 450
AYRHSYPHCW RHKTPIIFRA TPQWFISMDQ KGLRTQSLSE IEKTQWIPDW
460 470 480 490 500
GQSRIEKMVE NRPDWCISRQ RTWGVPITLF VHRDTEELHP DSVALMERVA
510 520 530 540 550
HRIEQEGIQA WWDLDAAELL GEEADQYRKV TDTLDVWYDS GSTFSSVVAA
560 570 580 590 600
RPEYNGHPVD LYLEGSDQHR GWFMSSLMIS TAMNGKAPYK QVLTHGFTVD
610 620 630 640 650
GQGRKMSKSI GNVIAPLQVT NKLGADILRL WVAATDYSGE MTVSDEILKR
660 670 680 690 700
SADAYRRIRN TARFLLANLN GFDPVNDTVA IEDMVALDRW VVRRAAALQA
710 720 730 740 750
EILEAYEQYN FHLVTQKLMQ FCSVELGSFY LDIIKDRQYT AKRDSLARRS
760 770 780 790 800
CQSALFLIAE AMVRWIAPIL SFTADEVWQS LPGEREEFVF TQTWFEGLVS
810 820 830 840 850
VTLDSDLSDD YWANLITVRD EVNKAIEQAR REQRVGGSLE AEVTIYADEA
860 870 880 890 900
LTESLALLGD ELRFVLLTSN TQIAPLANAG DDAVETELAG LKLLVDKTEA
910 920 930 940
AKCDRCWHHR EDVGQVEAHP LLCTRCVTNI EGDGEVRQFA
Length:940
Mass (Da):105,584
Last modified:April 17, 2007 - v1
Checksum:i5B315B649E3AA0E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000606 Genomic DNA. Translation: ABO22970.1.
RefSeqiYP_001093229.1. NC_009092.1.

Genome annotation databases

EnsemblBacteriaiABO22970; ABO22970; Shew_1099.
GeneIDi4923352.
KEGGislo:Shew_1099.
PATRICi23513975. VBISheLoi132094_1105.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000606 Genomic DNA. Translation: ABO22970.1.
RefSeqiYP_001093229.1. NC_009092.1.

3D structure databases

ProteinModelPortaliA3QBX2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323850.Shew_1099.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO22970; ABO22970; Shew_1099.
GeneIDi4923352.
KEGGislo:Shew_1099.
PATRICi23513975. VBISheLoi132094_1105.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciSLOI323850:GHQJ-1122-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1088 / PV-4.

Entry informationi

Entry nameiSYI_SHELP
AccessioniPrimary (citable) accession number: A3QBX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 17, 2007
Last modified: January 7, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.