Reviewed, UniProtKB/Swiss-Prot A3QAX7 (E4PD_SHELP)

Names and origin

Protein names

Recommended name:
    D-erythrose-4-phosphate dehydrogenase
      Short name=E4PDH
    EC=1.2.1.72

Gene names

Name:	epd
Ordered Locus Names:	Shew_0753

Organism

Shewanella loihica (strain ATCC BAA-1088 / PV-4) [Complete proteome] [HAMAP]

Taxonomic identifier

323850 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent conversion of D-erythrose 4-phosphate to 4-phosphoerythronate By similarity.
Catalytic activity	D-erythrose 4-phosphate + NAD(+) + H(2)O = 4-phosphoerythronate + NADH.
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. Epd subfamily.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridoxal phosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro erythrose-4-phosphate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	D-erythrose-4-phosphate dehydrogenase		PRO_0000293163
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	153 – 155	3	Substrate binding Potential
	Region	212 – 213	2	Substrate binding Potential
Sites
	Active site	154	1	Nucleophile By similarity
	Binding site	199	1	Substrate Potential
	Binding site	235	1	Substrate Potential
	Binding site	317	1	NAD By similarity
	Site	181	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3QAX7-1 [UniParc]. Last modified April 17, 2007. Version 1. Checksum: 0F99C35301D06BD0	FASTA	338	37,201
	10 20 30 40 50 60 MIKVAINGYG RIGRSILRAL YESGKRDQIQ IVAINELANP EAMLHLTQYD TTHGRFQSPA 70 80 90 100 110 120 SLDANVMTIG DDRIQLLHEA DAEKLPWRSL DIDIVFEATG ALIDRQACEA HIRAGAKQVL 130 140 150 160 170 180 ISHPSSGDVD ATIVYGVNHQ DLKAEHKVVS NASCTTNCLV PIIQVLDRAF KVRSGAITTI 190 200 210 220 230 240 HSAMNDQQVI DAYHDDLRRT RAAGQSIIPV DTKLARGIER ILPEMKDKFE AISVRVPTIN 250 260 270 280 290 300 VTAIDLSVTL DKRVDIDTVN QVLSQAANGS YQGVVGYTNA PLVSCDFNHD PRSSIVDGTQ 310 320 330 TRVSDGHLVK LLLWCDNEWG FANRMLDTSL EMIKAVRA

References

[1]

"Complete sequence of Shewanella loihica PV-4." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Romine M.F., Serres G., Fredrickson J., Tiedje J., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000606 Genomic DNA. Translation: ABO22625.1.
RefSeq	YP_001092884.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4923604.
GenomeReviews	Gene locus Shew_0753 in contig CP000606_GR.
KEGG	slo:Shew_0753.
NMPDR	fig|323850.3.peg.2929.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01640. [Tree]
InterPro	IPR006422. E4P_DHase_bac. IPR000173. GlycerAld_3-P_DHase. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01532. E4PD_g-proteo. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E4PD_SHELP

Accession

Primary (citable) accession number: A3QAX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	April 17, 2007
Last modified:	November 25, 2008
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)