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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadB

Organism
Shewanella loihica (strain ATCC BAA-1088 / PV-4)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.UniRule annotation

Catalytic activityi

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation
A (3Z)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.UniRule annotation
A (3E)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei119Important for catalytic activityUniRule annotation1
Sitei139Important for catalytic activityUniRule annotation1
Binding sitei296SubstrateUniRule annotation1
Binding sitei324NAD; via amide nitrogenUniRule annotation1
Binding sitei343NADUniRule annotation1
Binding sitei407NADUniRule annotation1
Binding sitei429NADUniRule annotation1
Active sitei450For 3-hydroxyacyl-CoA dehydrogenase activityUniRule annotation1
Binding sitei453NADUniRule annotation1
Binding sitei500SubstrateUniRule annotation1
Binding sitei660SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi400 – 402NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme, Oxidoreductase
Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism
LigandNAD

Enzyme and pathway databases

BioCyciSLOI323850:G1G8D-19-MONOMER
UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation, EC:5.3.3.8UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadBUniRule annotation
Ordered Locus Names:Shew_0019
OrganismiShewanella loihica (strain ATCC BAA-1088 / PV-4)
Taxonomic identifieri323850 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000001558 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000695761 – 716Fatty acid oxidation complex subunit alphaAdd BLAST716

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).UniRule annotation

Protein-protein interaction databases

STRINGi323850.Shew_0019

Structurei

3D structure databases

ProteinModelPortaliA3Q8U4
SMRiA3Q8U4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 189Enoyl-CoA hydratase/isomeraseUniRule annotationAdd BLAST189
Regioni311 – 7163-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST406

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DYT Bacteria
COG1024 LUCA
COG1250 LUCA
HOGENOMiHOG000261344
KOiK01825
OMAiDYKAKRQ
OrthoDBiPOG091H00UW

Family and domain databases

HAMAPiMF_01621 FadB, 1 hit
InterProiView protein in InterPro
IPR006180 3-OHacyl-CoA_DH_CS
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR001753 Enoyl-CoA_hydra/iso
IPR012799 FadB
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00725 3HCDH, 1 hit
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
TIGRFAMsiTIGR02437 FadB, 1 hit
PROSITEiView protein in PROSITE
PS00067 3HCDH, 1 hit

Sequencei

Sequence statusi: Complete.

A3Q8U4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYQSPTIQV ELLEDNIARL CFNAEGSVNK FDRETLNSLN DALDALAQTQ
60 70 80 90 100
GVKGLMLTSG KDAFIVGADI TEFLGLFAQD DNVLQGWLED ANKVFNKLED
110 120 130 140 150
LPFPTISAIK GFALGGGCET ILATDLRIAD TSARIGLPET KLGIIPGFGG
160 170 180 190 200
TVRLPRVIGA DNALEWITTG KDQRPEAALK VGAIDAVVAP ELLETAACQM
210 220 230 240 250
LQDAISEKID WQARRQRKLS PLTLPKLEAM MSFATAKGMV FKVAGKHYPA
260 270 280 290 300
PMAVVEVIEK AALSERAEAL QVEHQAFIKL AKTDVAKALI GIFLNDQLVK
310 320 330 340 350
GKAKKAAKQA QAVNSAAVLG AGIMGGGIAY QSASKGTPIV MKDINQAALD
360 370 380 390 400
LGLNEAAKLL TAQINRGRST PAKMAGVLNN ITATLDYNAL KQADVVVEAV
410 420 430 440 450
VEHPKVKATV LAEVEQVVGE DAIITSNTST ISINLLAKSL QKPERFCGMH
460 470 480 490 500
FFNPVHKMPL VEVIRGEHSS EETVASVVAY AAKMGKTPIV VNDCPGFFVN
510 520 530 540 550
RVLFPYFAGF SGLLEDGADF AAIDKVMEKQ FGWPMGPAYL LDVVGLDTGH
560 570 580 590 600
HAQAVMAEGF PDRMAKEGKD AIDVMFEADR FGQKNGKGFY QYSVDRRGKP
610 620 630 640 650
KKEVDPLSYE LLGNAFGEQK EFSSDEIIAR TMIPMIIETV RCLEEGIIAT
660 670 680 690 700
PAEADMGLVY GLGFPPFRGG VFRYLDTLGV ANFVALADQY AHLGGLYQVT
710
DKMRELAATN GSYYPA
Length:716
Mass (Da):77,144
Last modified:April 17, 2007 - v1
Checksum:i422E0A0B7AD430F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000606 Genomic DNA Translation: ABO21892.1
RefSeqiWP_011863829.1, NC_009092.1

Genome annotation databases

EnsemblBacteriaiABO21892; ABO21892; Shew_0019
KEGGislo:Shew_0019

Similar proteinsi

Entry informationi

Entry nameiFADB_SHELP
AccessioniPrimary (citable) accession number: A3Q8U4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: April 17, 2007
Last modified: April 25, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health