A3Q6H9 (OTSA_MYCSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trehalose-phosphate synthase
Short name=TPS
EC=2.4.1.-

Alternative name(s):
Trehalose-6-phosphate synthase

Gene names

Name:	otsA
Ordered Locus Names:	Mjls_4992

Organism

Mycobacterium sp. (strain JLS) [Complete proteome] [HAMAP]

Taxonomic identifier

164757 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6-phosphate and nucleoside diphosphate By similarity.
Catalytic activity	Nucleoside diphosphate-glucose + D-glucose 6-phosphate = trehalose 6-phosphate + nucleoside diphosphate.
Pathway	Glycan biosynthesis; trehalose biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glycosyltransferase 20 family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 483	483	Trehalose-phosphate synthase		PRO_0000348911
Regions
	Region	42 – 43	2	NDP-glucose binding By similarity
	Region	393 – 397	5	NDP-glucose binding By similarity
Sites
	Binding site	22	1	Glucose-6-phosphate By similarity
	Binding site	94	1	Glucose-6-phosphate By similarity
	Binding site	148	1	Glucose-6-phosphate By similarity
	Binding site	290	1	NDP-glucose By similarity
	Binding site	295	1	NDP-glucose By similarity
	Binding site	328	1	Glucose-6-phosphate By similarity
	Site	103	1	Involved in alpha anomer selectivity By similarity
	Site	173	1	Involved in alpha anomer selectivity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3Q6H9 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 5147F8DEC1C0F378	FASTA	483	54,203
	10 20 30 40 50 60 MAPEGDQRVG SGDSDFVVVA NRLPIDMERL PDGSTSIKRS PGGLVTALEP LLRKRRGAWI 70 80 90 100 110 120 GWAGIPDSPE DPIEEDGLQL YPVALSADDV ADYYEGFSNA TLWPLYHDLI VKPIYHRKWW 130 140 150 160 170 180 DRYVEVNRRF AEATARAAAQ GATVWVQDYQ LQLVPKMLRM LRPDLTIGFF LHIPFPPVEL 190 200 210 220 230 240 FMQMPWRTEI IEGLLGADLV GFHLPGGAQN FLYLSRRLTG ANTSRASVGV RSRFGEVQVG 250 260 270 280 290 300 FRTVKVGAFP ISIDSAELDS KARNRSVRQR AREIRNELGN PRKILLGVDR LDYTKGINVR 310 320 330 340 350 360 LEAFSELLED GRVDRNDTVL IQLATPSRER VESYIAMRED IERQVGHING EFGDVGHPIV 370 380 390 400 410 420 HYLHRPVPRD ELIAFFVAAD VMLVTPLRDG MNLVAKEYVA CRSDLGGALV LSEFTGAAAE 430 440 450 460 470 480 LRQAYLANPH HLEGVKDAIE AALNQDPEEG RRRMRALRRQ VLAHDVDRWA RAFLDALADT KSA

References

[1]

"Complete sequence of Mycobacterium sp. JLS." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Anderson A.J., Sims R.C., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JLS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000580 Genomic DNA. Translation: ABO00757.1.
RefSeq	YP_001073247.1. NC_009077.1.
3D structure databases
ProteinModelPortal	A3Q6H9.
ModBase	Search...
Protein-protein interaction databases
STRING	A3Q6H9.
Protein family/group databases
CAZy	GT20. Glycosyltransferase Family 20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000051200; EBMYCP00000049481; EBMYCG00000051195.
GeneID	4880690.
GenomeReviews	Gene locus Mjls_4992 in contig CP000580_GR.
KEGG	mjl:Mjls_4992.
PATRIC	18096237. VBIMycSp51234_5025.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0380.
GeneTree	EBGT00050000016525.
HOGENOM	HBG559076.
OMA	EYGRKEM.
PhylomeDB	A3Q6H9.
ProtClustDB	CLSK872185.
Enzyme and pathway databases
BioCyc	MSP164757:MJLS_4992-MONOMER.
Family and domain databases
InterPro	IPR001830. Glyco_trans_20. [Graphical view]
KO	K00697.
Pfam	PF00982. Glyco_transf_20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTSA_MYCSJ

Accession

Primary (citable) accession number: A3Q6H9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	April 3, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program