A3Q5H9 (A3Q5H9_MYCSJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
Short name=CMK HAMAP MF_00061
EC=2.7.1.148 HAMAP MF_00061

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase HAMAP MF_00061

Gene names

Name:	ispE HAMAP MF_00061
Ordered Locus Names:	Mjls_4641

Organism

Mycobacterium sp. (strain JLS) [Complete proteome] [HAMAP]

Taxonomic identifier

164757 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily. HAMAP MF_00061

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00061
Ligand	ATP-binding HAMAP MF_00061 RuleBase RU003563 Nucleotide-binding
Molecular function	Kinase HAMAP MF_00061 RuleBase RU003563 EMBL ABO00407.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	108 – 118	11	ATP By similarity HAMAP MF_00061
Sites
	Active site	23	1	By similarity HAMAP MF_00061
	Active site	150	1	By similarity HAMAP MF_00061

Sequences

	Sequence		Length	Mass (Da)
	A3Q5H9 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 6847502C9803ED36	FASTA	322	33,000
	10 20 30 40 50 60 MRNGNTASEW VPTGSVTVRV PGKVNLFLGV GDLRDDGYHD LTTVFHAVSL LDEVTVSTAD 70 80 90 100 110 120 TLSLDLAGEG AESLPTDRRN LAWKAAELMA EHVGRSPDVA ISIEKSIPVA GGMAGGSADA 130 140 150 160 170 180 AGVLVAMNHL WEIGVPRRDL HAMAAELGSD VPFALHGGTA LGTGRGEELA TVLARSTFHW 190 200 210 220 230 240 VLAFAYKGLS TPKVFRELDR LRATAGAARA EPARLGDPEP MLAALASGDA RQLAPLLGND 250 260 270 280 290 300 LQAAALSLSP DLRRTLRAGL DAGALAAIVS GSGPTCAFLC ASSPQAIDVG ARLAGAGVCR 310 320 TVRVASGPVQ GARVVPAPSP SV

References

[1]

"Complete sequence of Mycobacterium sp. JLS." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Anderson A.J., Sims R.C., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JLS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000580 Genomic DNA. Translation: ABO00407.1.
RefSeq	YP_001072897.1. NC_009077.1.
3D structure databases
ProteinModelPortal	A3Q5H9.
ModBase	Search...
Protein-protein interaction databases
STRING	A3Q5H9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000048697; EBMYCP00000046978; EBMYCG00000048692.
GeneID	4880340.
GenomeReviews	Gene locus Mjls_4641 in contig CP000580_GR.
KEGG	mjl:Mjls_4641.
PATRIC	18095500. VBIMycSp51234_4664.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
GeneTree	EBGT00050000016146.
HOGENOM	HBG734593.
OMA	KINLHLG.
PhylomeDB	A3Q5H9.
ProtClustDB	PRK03188.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A3Q5H9_MYCSJ

Accession

Primary (citable) accession number: A3Q5H9

Entry history

Integrated into UniProtKB/TrEMBL:	April 3, 2007
Last sequence update:	April 3, 2007
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)