A3Q379 (RNPH_MYCSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	Mjls_3830

Organism

Mycobacterium sp. (strain JLS) [Complete proteome] [HAMAP]

Taxonomic identifier

164757 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Ribonuclease PH HAMAP MF_00564		PRO_1000024831

Sequences

	Sequence		Length	Mass (Da)
	A3Q379 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 109A40CE4BB9A784	FASTA	260	27,647
	10 20 30 40 50 60 MSRREDGRLD DELRPVRITR GFTSHPAGSV LVEFGETRVM CTASVTEGVP RWRKGTGQGW 70 80 90 100 110 120 LTAEYAMLPA ATHDRSDRES VKGRVGGRTQ EISRLIGRSL RACIDLNALG ENTIAIDCDV 130 140 150 160 170 180 LQADGGTRTA AITGAYVALA DAVTYLAAAE KLSDPRPLSC AIAAVSVGVV DGRVRVDLPY 190 200 210 220 230 240 SEDSRAEVDM NVVATDTGTL VEIQGTGEGA TFPRSTLDKL LDLALASCDQ LFVVQREALD 250 260 APYPGALPEP TSPPKKAFGS

References

[1]

"Complete sequence of Mycobacterium sp. JLS." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Anderson A.J., Sims R.C., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JLS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000580 Genomic DNA. Translation: ABN99606.1.
RefSeq	YP_001072097.1. NC_009077.1.
3D structure databases
ProteinModelPortal	A3Q379.
SMR	A3Q379. Positions 3-248.
ModBase	Search...
Protein-protein interaction databases
STRING	A3Q379.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000051492; EBMYCP00000049773; EBMYCG00000051487.
GeneID	4879540.
GenomeReviews	Gene locus Mjls_3830 in contig CP000580_GR.
KEGG	mjl:Mjls_3830.
PATRIC	18093884. VBIMycSp51234_3861.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0689.
GeneTree	EBGT00050000017525.
HOGENOM	HBG737187.
OMA	MLPRATG.
PhylomeDB	A3Q379.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	MSP164757:MJLS_3830-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_MYCSJ

Accession

Primary (citable) accession number: A3Q379

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program